An osmotic micro-pump integrated on a microfluidic chip for perfusion cell culture

Xu, Zhang-Run; Yang, Chunguang; Liu, Cuihong; Zhou, Zhe; Jin, Fang; Wang, Jianhua

doi:10.1016/j.talanta.2009.08.031

Cited by 45 publications

(31 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternatively, to further simplify the device, the syringe pump could be replaced by a gravity-driven micro-pump. 62 We also plan to include a mechanism for extraction of the cell aggregates in order to extend the currently enabled screening studies on the Polyester l-well chip to experiments focused on stem cell biology. Such a mechanism could be based on peeling off the PDMS layer and looping the cell aggregates out with a metal wire, as is commonly done with protein crystals, 63,64 or applying negative pressure to one particular channel to suck out all aggregates cultured at the same experimental condition.…”

Section: Resultsmentioning

confidence: 99%

Polyester μ-assay chip for stem cell studies

et al. 2012

View full text Add to dashboard Cite

The application of microfluidic technologies to stem cell research is of great interest to biologists and bioengineers. This is chiefly due to the intricate ability to control the cellular environment, the reduction of reagent volume, experimentation time and cost, and the high-throughput screening capabilities of microscale devices. Despite this importance, a simple-to-use microfluidic platform for studying the effects of growth factors on stem cell differentiation has not yet emerged. With this consideration, we have designed and characterized a microfluidic device that is easy to fabricate and operate, yet contains several functional elements. Our device is a simple polyester-based microfluidic chip capable of simultaneously screening multiple independent stem cell culture conditions. Generated by laser ablation and stacking of multiple layers of polyester film, this device integrates a 10 Â 10 microwell array for cell culture with a continuous perfusion system and a non-linear concentration gradient generator. We performed numerical calculations to predict the gradient formation and calculate the shear stress acting on the cells inside the device. The device operation was validated by culturing murine embryonic stem cells inside the microwells for 5 days. Furthermore, we showed the ability to maintain the pluripotency of stem cell aggregates in response to concentrations of leukemia inhibitory factor ranging from 0 to $1000 U/ml. Given its simplicity, fast manufacturing method, scalability, and the cell-compatible nature of the device, it may be a useful platform for long-term stem cell culture and studies.

show abstract

Section: Resultsmentioning

confidence: 99%

Polyester μ-assay chip for stem cell studies

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Not only does this novel microfluidic culture platform better mimic in vivo flow conditions, media recirculation economizes culture media and treatment compounds 52 while significantly reducing cost. Additionally, when a multimodality imaging system 53,54 is coupled with the culture platform, data can be collected on morphological cell changes under dynamic conditions/ treatments.…”

Section: Effects Of Diode Pump-driven Flows On Cellsmentioning

confidence: 99%

A novel miniature dynamic microfluidic cell culture platform using electro-osmosis diode pumping

et al. 2014

View full text Add to dashboard Cite

An electro-osmosis (EOS) diode pumping platform capable of culturing cells in fluidic cellular micro-environments particularly at low volume flow rates has been developed. Diode pumps have been shown to be a viable alternative to mechanically driven pumps. Typically electrokinetic micro-pumps were limited to lowconcentration solutions ( 10 mM). In our approach, surface mount diodes were embedded along the sidewalls of a microchannel to rectify externally applied alternating current into pulsed direct current power across the diodes in order to generate EOS flows. This approach has for the first time generated flows at ultra-low flow rates (from 2.0 nl/s to 12.3 nl/s) in aqueous solutions with concentrations greater than 100 mM. The range of flow was generated by changing the electric field strength applied to the diodes from 0.5 Vpp/cm to 10 Vpp/cm. Embedding an additional diode on the upper surface of the enclosed microchannel increased flow rates further. We characterized the diode pump-driven fluidics in terms of intensities and frequencies of electric inputs, pH values of solutions, and solution types. As part of this study, we found that the growth of A549 human lung cancer cells was positively affected in the microfluidic diode pumping system. Though the chemical reaction compromised the fluidic control overtime, the system could be maintained fully functional over a long time if the solution was changed every hour. In conclusion, the advantage of miniature size and ability to accurately control fluids at ultra-low volume flow rates can make this diode pumping system attractive to lab-on-a-chip applications and biomedical engineering in vitro studies. V C 2014 AIP Publishing LLC. [http://dx

show abstract

“…Disadvantages of these methods include low production, large sample losses, difficult separation, complicated operation, and limited safety. Recently [4][5][6][7][8][9] cell lysis methods based on microfluidic technology have been employed to eliminate these limitations [10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%