Design and application of a microfluidic cell lysis microelectrode chip

Jiang, Feng; Chen, Jianjun; Yu, Jun

doi:10.1080/10739149.2015.1076839

Cited by 9 publications

(7 citation statements)

References 21 publications

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“…Once the potential exceeds a certain threshold, pores form on the membrane to release intracellular components. Unlike the detergents utilized in chemical lysis, it is known that there are no effects on the intracellular contents after application of an electric field high 4 [ 57 , 58 ]. Electroporation-based devices do not differ from mechanical- and chemical-based devices regarding their flow drive mechanisms.…”

Section: Cell Lysis Methodsmentioning

confidence: 99%

Review of Microfluidic Methods for Cellular Lysis

et al. 2021

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Cell lysis is a process in which the outer cell membrane is broken to release intracellular constituents in a way that important information about the DNA or RNA of an organism can be obtained. This article is a thorough review of reported methods for the achievement of effective cellular boundaries disintegration, together with their technological peculiarities and instrumental requirements. The different approaches are summarized in six categories: chemical, mechanical, electrical methods, thermal, laser, and other lysis methods. Based on the results derived from each of the investigated reports, we outline the advantages and disadvantages of those techniques. Although the choice of a suitable method is highly dependent on the particular requirements of the specific scientific problem, we conclude with a concise table where the benefits of every approach are compared, based on criteria such as cost, efficiency, and difficulty.

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Section: Cell Lysis Methodsmentioning

confidence: 99%

Review of Microfluidic Methods for Cellular Lysis

et al. 2021

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“…Jiang et al [ 88 ] developed a low cost microfluidic device for cell lysis using electric fields. They applied a 10 V square pulse to lyse cells at 50% efficiency.…”

Section: Microfabricated Platforms For Cell Lysismentioning

confidence: 99%

A Review on Macroscale and Microscale Cell Lysis Methods

2017

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The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.

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“…With this method, cells were lysed in a continuous-flow device in small volumes with a lower power consumption than in conventional larger equipment [ 14 ]. Furthermore, Jiang et al used an electric signal to lyse their cells; a 6.8 V square wave pulse resulted in a lysis efficiency of about 50% [ 17 ].…”

Section: Sample Work-upmentioning

confidence: 99%

Microfluidic Devices for Forensic DNA Analysis: A Review

et al. 2016

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Microfluidic devices may offer various advantages for forensic DNA analysis, such as reduced risk of contamination, shorter analysis time and direct application at the crime scene. Microfluidic chip technology has already proven to be functional and effective within medical applications, such as for point-of-care use. In the forensic field, one may expect microfluidic technology to become particularly relevant for the analysis of biological traces containing human DNA. This would require a number of consecutive steps, including sample work up, DNA amplification and detection, as well as secure storage of the sample. This article provides an extensive overview of microfluidic devices for cell lysis, DNA extraction and purification, DNA amplification and detection and analysis techniques for DNA. Topics to be discussed are polymerase chain reaction (PCR) on-chip, digital PCR (dPCR), isothermal amplification on-chip, chip materials, integrated devices and commercially available techniques. A critical overview of the opportunities and challenges of the use of chips is discussed, and developments made in forensic DNA analysis over the past 10–20 years with microfluidic systems are described. Areas in which further research is needed are indicated in a future outlook.

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Design and application of a microfluidic cell lysis microelectrode chip

Cited by 9 publications

References 21 publications

Review of Microfluidic Methods for Cellular Lysis

Review of Microfluidic Methods for Cellular Lysis

A Review on Macroscale and Microscale Cell Lysis Methods

Microfluidic Devices for Forensic DNA Analysis: A Review

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