An on-site immunoassay for detecting PCB in soil

Mapes, James P.; McKenzie, Karen D.; Stewart, Thomas N.; McClelland, Lisa R.; Studabaker, William B.; Manning, Wayne B.; Friedman, Stephen B.

doi:10.1007/bf00191725

Cited by 23 publications

(9 citation statements)

References 4 publications

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“…Other immunoassays have also been reported to detect Aroclors or the most abundant and also less toxic noncoplanar PCBs in soil, water, and sediments (see Table 1 in ref. [122]) [125,126,127,128].…”

Section: Immunochemical Techniques For Pcbsmentioning

confidence: 98%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

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This paper is a review with more than 100 references discussing the immunochemical methods reported in the literature for the most important man-made chemicals with suspected endocrine disrupting activity. Details regarding immunizing hapten design, antibody production, and the features (limit of detection, dynamic range, specificity) of the most important immunochemical methods developed (ELISA, FIIA, immunosorbents, immunosensors, etc.) are presented for important environmental pollutants such as bisphenol A, phthalates, alkylphenol polyethoxylates, alkylphenols, polychlorinated biphenyl compounds, and dioxins. Availability of commercial reagents and methods is reported.

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Section: Immunochemical Techniques For Pcbsmentioning

confidence: 98%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

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“…In the early days, soil and sediment samples were easily extracted by shaking with methanol or/and Soxhlet [47,54,55] or using an ultrasonic process [56], but these methods did not result in a complete extraction, leading to compound loss or the need for further cleanup. In order to solve these problems, several alternative extraction technologies were introduced, which resulted in time and reagent savings.…”

Section: Detection Methodsmentioning

confidence: 99%

“…Polyclonal antibodies are widely used and easy to be obtained and purified from serum. For example, Mapes et al [54] utilized New Zealand white rabbit to raise polyclonal antibodies against PCBs in the development of a fast on-site immunoassay. By using this assay PCBs in soil could be detected at concentrations as low as 5 ppm.…”

Section: Detection Methodsmentioning

confidence: 99%

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Tian

Xie

et al. 2012

Sensors

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With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.

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“…2 Hence, it has been listed as a priority pollutant by the US Environmental Protection Agency (EPA) and classied as a group 2B environmental carcinogen by the International Association for Research on Cancer (IARC). [3][4][5] A rapid and sensitive analytical method to determine trace level of PCP is therefore needed, and various analytical schemes have been proposed, such as gas chromatography (GC), [6][7][8][9] gas chromatography-mass spectrometry (GC-MS), 10 high performance liquid chromatography (HPLC), [11][12][13] thin layer chromatography, 14 phosphorescence spectrometry, 15 spectrophotometry 16 and electrochemistry. 2 Most of these methods are either expensive, time-consuming and labor-intensive, or require highly qualied personnel and sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

Electrogenerated chemiluminescence detection of trace level pentachlorophenol using carbon quantum dots

Wang

Tran.T

et al. 2013

Analyst

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Pentachlorophenol (PCP) is an environmental pollutant of serious concern due to its high toxicity and long persistence property. Fast and sensitive detection of PCP is therefore of great interest. In this work, carbon quantum dots (QDs) are synthesized by hydrothermal reaction, and characterized by fluorescence spectrophotometer, fourier transform infrared (FT-IR) spectroscopy, UV/vis/NIR spectrophotometer and scanning electron microscopy (SEM). The carbon QDs show stable and intensive electrogenerated chemiluminescence (ECL) in the presence of the coreactant S2O8(2-). Under the scanning potential of -1.2 to 0.5 V, PCP reacts with the excited C˙(-), resulting in a decrease in ECL. The detection of trace level PCP is therefore achieved using the carbon QDs and Pt working electrode. Parameters that may affect the ECL intensity including the pH of solution, ionic strength, concentrations of coreactant and carbon QDs are optimized. Under the optimal conditions, a detection limit of 1.3 × 10(-12) g L(-1) is achieved with a linear range of 10 pg L(-1)∼1.0 μg L(-1).

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An on-site immunoassay for detecting PCB in soil

Cited by 23 publications

References 4 publications

Immunochemical determination of xenobiotics with endocrine disrupting effects

Immunochemical determination of xenobiotics with endocrine disrupting effects

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Electrogenerated chemiluminescence detection of trace level pentachlorophenol using carbon quantum dots

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