Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Tian, Wenjing; Xie, Heidi Qunhui; Fu, Hualing; Pei, Xinhui; Zhao, Baojun

doi:10.3390/s121216710

Cited by 17 publications

(25 citation statements)

References 118 publications

(97 reference statements)

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“…Such bioassays are inexpensive and rapid methods for the specic analysis of adopted hazards and critical control points, which have a high potential application to monitor marine and coastal sources, including seafood. 57,58 This system is known as a quite sensitive method to detect the presence of Ah receptor agonists and biological effects of active congeners at the AhR level. Receptor binding.…”

Section: In Vitro Bioassaysmentioning

confidence: 99%

“…8,47 4.3.1. 31,[56][57][58] Unfortunately, these assays do not necessarily distinguish receptor antagonists and AhR agonists and providing false positive data. Various types of in vitro bioassays are developed to monitor dioxin like compounds.…”

Section: In Vitro Bioassaysmentioning

confidence: 99%

“…Various types of in vitro bioassays are developed to monitor dioxin like compounds. 31,58,88 Another problem is in the quality control of the gel retardation methods which make them unreliable. This is known as a competitive binding method by using [ 3 H]TCDD and [ 125 I]2iodo-7,8-dibromodibenzo-p-dioxin binding agents.…”

Section: In Vitro Bioassaysmentioning

confidence: 99%

“…8,59,95 A. AHH/EROD bioassay. 58,102 In the CAFLUX assay, persistent and non-persistent AhR agonists produce a cumulative signal. 31,47 In this method, activities of several CYPs are estimated based on the different substrates, for CYP1A2 (methoxyresorun-O-demethylase; MROD), for CYP1A1 (EROD), and for CYP2B1 (pentoxyresorun-O-depenthylase; PROD).…”

Section: In Vitro Bioassaysmentioning

confidence: 99%

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An overview of detection techniques for monitoring dioxin-like compounds: latest technique trends and their applications

et al. 2016

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Dioxin-like compounds (DLCs) are considered persistent bioaccumulative toxicants with a number of continuing issues in the fields of ecotoxicology and bioassay. In spite of the great need to monitor these compounds, the only analytical technique with sufficient sensitivity and selectivity for determination of DLCs is a combination of high-resolution gas chromatography and high-resolution mass spectrometry.However, these methods require aseptic techniques, long incubation times, and sophisticated technical expertise for getting accurate results. Nowadays, biological techniques such as biomarkers, bioassays, enzyme immunoassays (EIAs), or other methods have been greatly developed as more sensitive, costeffective and rapid techniques to determine the presence of DLCs in trace levels of environmental and biological samples. The main aim of this study is to review the latest analytical and bioanalytical detection methods (BDMs) for diagnosis and monitoring of DLCs. Likewise, this work characterizes the latest techniques and trends based on their application, advantages and shortcomings for the various BDMs and their differences are also noted.

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Section: In Vitro Bioassaysmentioning

confidence: 99%

Section: In Vitro Bioassaysmentioning

confidence: 99%

Section: In Vitro Bioassaysmentioning

confidence: 99%

Section: In Vitro Bioassaysmentioning

confidence: 99%

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An overview of detection techniques for monitoring dioxin-like compounds: latest technique trends and their applications

et al. 2016

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“…Due to the lack of 3D crystal or nuclear magnetic resonance structure of AhR, monoclonal antibodies (mAbs), binding specifically to various functional domains of AhR, could serve as effective tools for sophisticated studies on the molecular events and mechanisms. Apart from that, mAbs can also be used to detect AhR ligands in the environment, such as in Ah-Immunoassay (Ah-I) (Tian et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor

Tian

Pei

Xie

et al. 2016

Journal of Environmental Sciences

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Aryl hydrocarbon receptor (AhR), a ligand-dependent nuclear receptor, is involved in a diverse spectrum of biological and toxicological effects. Due to the lack of three dimensional (3D) crystal or nuclear magnetic resonance structure, the mechanisms of these complex effects of AhR remain to be unclear. Also, commercial monoclonal antibodies (mAbs) against human AhR protein (hAhR), as alternative immunological tools, are very limited. Thus, in order to provide more tools for further studies on hAhR, we prepared two mAbs (1D6 and 4A6) against hAhR. The two newly generated mAbs specifically bound to amino acids 484-508 (located in transcription activation domain) and amino acids 201-215 (located in Per-ARNT-Sim domain) of hAhR, respectively. These epitopes were new as compared with those of commercial mAbs.The mAbs were also characterized by enzyme-linked immunosorbent assay, western blot, immunoprecipitation and indirect immunofluorescence assay in different cell lines. The results showed that the two mAbs could recognize the linearized AhRs in six different human cell lines and a rat hepatoma cell line, as well as the hAhR with native conformations. We concluded that the newly generated mAbs could be employed in AhR-based bioassays for analysis of environmental contaminants, and held great potential for further revealing the spatial structure of AhR and its biological functions in future studies.

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Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box

Otarola

Castillo

Marcellini

2017

J of Applied Toxicology

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Despite intensive media coverage and international regulations, man-made persistent organic pollutants such as dioxins represent a serious environmental and health threat. Their detection by sophisticated chromatography technologies is highly complex, impeding the constant monitoring of food or environmental samples. This limitation has fostered the development of generations of bioassays exploiting the molecular function of the aryl hydrocarbon receptor (AhR), which binds toxic compounds and directly activates the transcription of target genes. Here, we review the rich panel of available AhR-dependent bioassays and propose a novel classification based on the source of AhR, which can either be endogenously produced by cell types or tissues naturally responsive to dioxins, or exogenously introduced into a wide range of cellular contexts. In both cases, in vitro and in vivo strategies have been engineered to monitor the formation of molecular complexes, and the activation of direct downstream targets or reporter genes. We evaluate and compare bioassays based on exogenous and endogenous AhR proteins and discuss their specific challenges, strengths and opportunities for futures applications. Undoubtedly, the dynamic field of AhR-dependent bioassays will keep providing new and original strategies to help protect human health and ecosystems from persistent organic pollutants.

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Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Cited by 17 publications

References 118 publications

An overview of detection techniques for monitoring dioxin-like compounds: latest technique trends and their applications

An overview of detection techniques for monitoring dioxin-like compounds: latest technique trends and their applications

Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor

Aryl hydrocarbon receptor‐based bioassays for dioxin detection: Thinking outside the box

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