An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe

Liang, Ruqiang; Li, Wei; Li, Yang; Tan, Cuiyan; Li, Jianxun; Jin, Youxin; Ruan, Kangcheng

doi:10.1093/nar/gni019

Cited by 303 publications

(134 citation statements)

References 31 publications

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“…miRNAspecific quantitive PCR and microarray technology make this study become easier. In the past 1 year, several quantitive PCR Raymond et al, 2005;Shi and Chiang, 2005) and microarray technology (Babak et al, 2004;Barad et al, 2004;Nelson et al, 2004b;Thomson et al, 2004;Liang et al, 2005) have been developed, and successfully used to study the functions of miRNAs in organism development (Miska et al, 2004), disease and cancer (Lu et al, 2005b). At present, several companies have developed miRNA chips for studying the expression profiles at a specific situation.…”

Section: Historymentioning

confidence: 99%

“…Knockdown and/or knockout or overexpression of specific miRNAs is a good approach to investigate the specific function of an unique miRNA (Hutvagner et al, 2004;Meister et al, 2004a;Lee et al, 2005). Recently developed miRNA microarray technology and miRNAspecific real-time PCR also provide useful information on miRNA functions (Miska et al, 2004;Baskerville and Bartel, 2005;Liang et al, 2005).…”

Section: Functions Of Micrornas In Animalsmentioning

confidence: 99%

“…The first evidence for miRNAs involved in cancer came from a molecular study characterizing the 13q14 deletion in human chronic lymphocytic leukemia (CLL) (Calin et al, 2002), the most common form of adult leukemia in the Western world (Dohner et al, 2000). With the development of mature miRNA microarray technology (Babak et al, 2004;Barad et al, 2004;Liu et al, 2004;Nelson et al, 2004a,b;Thomson et al, 2004;Liang et al, 2005;Lu et al, 2005b), more and more evidences become available to connect miRNAs with cancers.…”

Section: Functions Of Micrornas In Animalsmentioning

confidence: 99%

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MicroRNAs and their regulatory roles in animals and plants

Zhang

Wang

Pan

2006

Journal Cellular Physiology

485

307

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microRNAs (miRNAs) are an abundant class of newly identified endogenous non-protein-coding small RNAs. They exist in animals, plants, and viruses, and play an important role in gene silencing. Translational repression, mRNA cleavage, and mRNA decay initiated by miRNA-directed deadenylation of targeted mRNAs are three mechanisms of miRNA-guided gene regulation at the posttranscriptional levels. Many miRNAs are highly conserved in animals and plants, suggesting that they play an essential function in plants and animals. Lots of investigations indicate that miRNAs are involved in multiple biological processes, including stem cell differentiation, organ development, phase change, signaling, disease, cancer, and response to biotic and abiotic environmental stresses. This review provides a general background and current advance on the discovery, history, biogenesis, genomics, mechanisms, and functions of miRNAs.

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Section: Historymentioning

confidence: 99%

Section: Functions Of Micrornas In Animalsmentioning

confidence: 99%

Section: Functions Of Micrornas In Animalsmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNAs and their regulatory roles in animals and plants

Zhang

Wang

Pan

2006

Journal Cellular Physiology

485

307

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“…Several different approaches enabling multiplexed detection of miRNAs using microarray technologies have been reported (Krichevsky et al, 2003;Babak et al, 2004;Liu et al, 2004;Miska et al, 2004;Nelson et al, 2004;Sun et al, 2004;Thomson et al, 2004;Liang et al, 2005), including one from our laboratory (Baskerville and Bartel, 2005). In our approach, probes consist of T m -normalized DNA oligonucleotides antisense to the given small RNA sequence.…”

Section: Introductionmentioning

confidence: 99%

Antiquity of MicroRNAs and Their Targets in Land Plants

2005

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MicroRNAs (miRNAs) affect the morphology of flowering plants by the posttranscriptional regulation of genes involved in critical developmental events. Understanding the spatial and temporal dynamics of miRNA activity during development is therefore central for understanding miRNA functions. We describe a microarray suitable for detection of plant miRNAs. Profiling of Arabidopsis thaliana miRNAs during normal development extends previous expression analyses, highlighting differential expression of miRNA families within specific organs and tissue types. Comparison of our miRNA expression data with existing mRNA microarray data provided a global intersection of plant miRNA and mRNA expression profiles and revealed that tissues in which a given miRNA is highly expressed are unlikely to also show high expression of the corresponding targets. Expression profiling was also used in a phylogenetic survey to test the depth of plant miRNA conservation. Of the 23 families of miRNAs tested, expression of 11 was detected in a gymnosperm and eight in a fern, directly demonstrating that many plant miRNAs have remained essentially unchanged since before the emergence of flowering plants. We also describe an empirical strategy for detecting miRNA target genes from unsequenced transcriptomes and show that targets in nonflowering plants as deeply branching as ferns and mosses are homologous to the targets in Arabidopsis. Therefore, several individual miRNA regulatory circuits have ancient origins and have remained intact throughout the evolution and diversification of plants.

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“…It has been assumed that most, if not all, biosensing measurements rely on the information of the final complex followed by host-guest molecules association. Nevertheless, several binding events cannot form a stable complex, and the binding process is dynamic, even reversible, with a weak binding strength, which poses a great challenge for routine detection tools, like microarray that involves tedious fluorescent labeling and is generally incapable of real-time monitoring (Liang et al 2005, Li et al 2010). Thus, a reliable, label-free, and real-time transduction strategy for molecular interactions is highly desirable.…”

Section: Introductionmentioning

confidence: 99%

Silicon nanowire-transistor biosensor for study of molecule-molecule interactions

Yang

Zhang

2014

Reviews in Analytical Chemistry

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Abstract:Monitoring the molecular recognition, binding, and disassociation between probe and target is important in medical diagnostics and drug screening, because such a wealth of information can be used to identify the pathogenic species and new therapeutic candidates. Nanoelectronic biosensors based on silicon nanowire field-effect transistors (SiNW-FETs) have recently attracted tremendous attention as a promising tool in the investigation of biomolecular interactions due to their capability of ultrasensitive, selective, real-time, and label-free detection. Herein, we summarize the recent advances in label-free analysis of molecule-molecule interactions using SiNWFETs, with a discussion and emphasis on small molecule-biomolecule interaction, biomolecule-biomolecule interactions (including carbohydrate-protein interaction, protein-protein or antigen-antibody binding, and nucleic acid-nucleic acid hybridization), and protein-virus interaction. Such molecular recognitions offer a basis of biosensing and the dynamics assay of biomolecular association or dissociation. Compared to the conventional technologies, SiNW-FETs hold great promise to monitor molecule-molecule interactions with higher sensitivity and selectivity. Finally, several prospects concerning the future development of SiNW-FET biosensor are discussed.

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An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe

Cited by 303 publications

References 31 publications

MicroRNAs and their regulatory roles in animals and plants

MicroRNAs and their regulatory roles in animals and plants

Antiquity of MicroRNAs and Their Targets in Land Plants

Silicon nanowire-transistor biosensor for study of molecule-molecule interactions

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