An NMR-based metabolomic assessment of cultured cobia health in response to dietary manipulation

Schock, Tracey B.; Newton, Sarah; Brenkert, Karl; Leffler, John W.; Bearden, Daniel W.

doi:10.1016/j.foodchem.2011.12.077

Cited by 54 publications

(65 citation statements)

References 33 publications

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“…The metabolome is affected by multiple factors including environmental conditions as well as diet. 7, 15, 16 Metabolomics has been successfully applied to studies focused on assessing the response of aquatic species to different stressors (e.g. toxicity, disease, temperature and diet).…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Analysis of Effects of Commercial Soy-based Protein Products in Red Drum (Sciaenops ocellatus)

et al. 2017

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In this study, we investigated the metabolic effects of four different commercial soy-based protein products on red drum fish (Sciaenops ocellatus) using Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomics along with unsupervised principal component analysis (PCA) to evaluate metabolic profiles in liver, muscle and plasma tissues. Specifically, during a 12-week feeding trial, juvenile red drum maintained in an indoor recirculating aquaculture system were fed four different commercially available soy formulations, containing the same amount of crude protein, and two reference diets as performance controls: a 60 % soybean meal diet that had been used in a previous trial in our lab and a natural diet. Red drum liver, muscle, and plasma tissues were sampled at multiple time points to provide a more accurate snapshot of specific metabolic states during the grow-out. PCA score plots derived from NMR spectroscopy data sets showed significant differences between fish fed the natural diet and the soy-based diets, both in liver and muscle tissues. While red drum tolerated the inclusion of soy with good feed conversion ratios, a comparison to fish fed the natural diet revealed that the soy-fed fish in this study displayed a distinct metabolic signature characterized by increased protein and lipid catabolism, suggesting an energetic imbalance. Furthermore, among the soy-based formulations, one diet showed a more pronounced catabolic signature.

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Section: Introductionmentioning

confidence: 99%

Metabolomics Analysis of Effects of Commercial Soy-based Protein Products in Red Drum (Sciaenops ocellatus)

et al. 2017

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“…These analyses tend to centre around mass spectrometry (MS), often in combination with chromatography, but the use of nuclear magnetic resonance (NMR; e.g. Schock et al 2012;Villa et al 2013) can also be informative.…”

Section: Metabolomicsmentioning

confidence: 99%

“…The retrieved metabolomics-related studies were valuable for identification of impacts of husbandry (stress; Picone et al 2011) and diet (Schock et al 2012). In addition, product quality analysis, for example, in response to different storage conditions, can benefit from such an approach.…”

Section: Metabolomicsmentioning

confidence: 99%

Molecular and biochemical tricks of the research trade: -omics approaches in finfish aquaculture

Lokman

Symonds

2014

New Zealand Journal of Marine and Freshwater Research

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Profitability of fish farming can be undermined by a suite of biological constraints. Effects of these constraints on the biology of the animal, the functioning of a tissue, or the quality of the final filleted product can be detected with very sensitive hi-tech approaches, grouped by the term '-omics'. These -omics technologies further present powerful tools for the genetic selection of diverse traits and, as such, will contribute substantially to the future development of the aquaculture sector. Sustainability issues of importance to the industry, such as environmental impacts, use of therapeutants, fish health management and fishmeal replacement, can again be aided, at least in part, through the effective use of -omics technologies. It thus appears that -omics technologies may be a key tool for the responsible growth of intensive aquaculture in the 21st century. In this paper we review the current status of -omics technologies and the potential for application within the aquaculture industry.

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“…Metabolomics has emerged as a powerful technique to study fish biology, including applications in toxicology [1,2,3,4], nutrition [5,6] and disease [7,8,9]. Further studies have characterised the metabolic changes associated with fundamental processes such as fish embryogenesis [10,11].…”

Section: Introductionmentioning

confidence: 99%

Application of Passive Sampling to Characterise the Fish Exometabolome

et al. 2017

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The endogenous metabolites excreted by organisms into their surrounding environment, termed the exometabolome, are important for many processes including chemical communication. In fish biology, such metabolites are also known to be informative markers of physiological status. While metabolomics is increasingly used to investigate the endogenous biochemistry of organisms, no non-targeted studies of the metabolic complexity of fish exometabolomes have been reported to date. In environmental chemistry, Chemcatcher® (Portsmouth, UK) passive samplers have been developed to sample for micro-pollutants in water. Given the importance of the fish exometabolome, we sought to evaluate the capability of Chemcatcher® samplers to capture a broad spectrum of endogenous metabolites excreted by fish and to measure these using non-targeted direct infusion mass spectrometry metabolomics. The capabilities of C18 and styrene divinylbenzene reversed-phase sulfonated (SDB-RPS) Empore™ disks for capturing non-polar and polar metabolites, respectively, were compared. Furthermore, we investigated real, complex metabolite mixtures excreted from two model fish species, rainbow trout (Oncorhynchus mykiss) and three-spined stickleback (Gasterosteus aculeatus). In total, 344 biological samples and 28 QC samples were analysed, revealing 646 and 215 m/z peaks from trout and stickleback, respectively. The measured exometabolomes were principally affected by the type of Empore™ (Hemel Hempstead, UK) disk and also by the sampling time. Many peaks were putatively annotated, including several bile acids (e.g., chenodeoxycholate, taurocholate, glycocholate, glycolithocholate, glycochenodeoxycholate, glycodeoxycholate). Collectively these observations show the ability of Chemcatcher® passive samplers to capture endogenous metabolites excreted from fish.

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An NMR-based metabolomic assessment of cultured cobia health in response to dietary manipulation

Cited by 54 publications

References 33 publications

Metabolomics Analysis of Effects of Commercial Soy-based Protein Products in Red Drum (Sciaenops ocellatus)

Metabolomics Analysis of Effects of Commercial Soy-based Protein Products in Red Drum (Sciaenops ocellatus)

Molecular and biochemical tricks of the research trade: -omics approaches in finfish aquaculture

Application of Passive Sampling to Characterise the Fish Exometabolome

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