An isocratic liquid chromatography method for determining HIV non-nucleoside reverse transcriptase inhibitor and protease inhibitor concentrations in human plasma

Weller, Dennis R.; Brundage, Richard C.; Balfour, Henry H.; Vezina, Heather

doi:10.1016/j.jchromb.2006.10.022

Cited by 41 publications

(30 citation statements)

References 12 publications

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“…This new drug is official in IP -2010, but not included in BP or USP. The reported analytical methods for the determination of ATV are based on high performance liquid chromatography (HPLC) [3][4][5][6][7][8][9][10][11][12] in biological samples like blood plasma, biological cells, cerebrospinal fluid (CSF) and blood serum. Stress degradation studies were reported analysed by HPLC and ultraviolet spectrophotometry.…”

Section: Atazanavir Sulfate (Atv) Methyl N′-[(1s)-1-{n-[(2s3s)-mentioning

confidence: 99%

Spectrophotometric method for determination of atazanavir sulfate in capsule dosage form

Behera¹,

Moitra²,

Chandra³

et al. 2011

Quím. Nova

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Recebido em 19/11/10; aceito em 2/4/11; publicado na web em 10/6/11Two sensitive and simple spectrophotmetric methods were developed for determination of Atazanavir Sulfate in capsule dosage form. The first method was based on the oxidative coupling of ATV with 3-Methyl Benzothiazolin-2-one hydrazone (MBTH). The resulting green product had λ max of 627.3 nm and was stable for 2 h. The second method was based on the reaction between diazotized drug with N-(1-napthyl)ethylenediamine dihydrochloride (NEDA) in neutral medium to yield yellowish orange product which had λ max of 517.1 nm. The product was stable for 4 h. Both methods were highly reproducible and had been applied to pharmaceutical preparations.

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Section: Atazanavir Sulfate (Atv) Methyl N′-[(1s)-1-{n-[(2s3s)-mentioning

confidence: 99%

Spectrophotometric method for determination of atazanavir sulfate in capsule dosage form

Behera¹,

Moitra²,

Chandra³

et al. 2011

Quím. Nova

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“…On the contrary, reverse phase highperformance liquid chromatographic with ultraviolet detector (RP-HPLC-UV) method is feasible due to its easy accessibility and low cost. Also, many HPLC-UV methods for determination of plasma efavirenz (23,(26)(27)(28)(29)(30)(31) or simultaneous determination with other drugs such as anti-tuberculosis (anti-TB) agents or other antiretroviral agents (24,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) have been reported. In most of these methods, the volumes of plasma used range from 200 to 900 μL (23,(26)(27)(28)33,34,(44)(45)(46), which were inapplicable for children due to the scarcity of sample.…”

Section: Introductionmentioning

confidence: 99%

A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

Yin

Meng

Dong

et al. 2014

BST

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“…There are several methods described in the literature for the quantitative analysis of atazanavir in plasma, either alone [5][6][7] or in combination with other ARVs [7][8][9][10]. Some authors reported the use of mass spectrometry for detection [10], which is not routinely available in all laboratories.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study

Singh¹

2014

J Chromat Separation Techniq

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A new reverse phase liquid chromatographic method for the investigation of atazanavir in rat plasma was developed after oral administration. The chromatographic separation was achieved on Phenomenex C 18 column (250 mm × 4.6 mm I.D., 5 μm), under isocratic conditions using UV detection at 249 nm. The optimized mobile phase consisted of a mixture of potassium dihydrogen phosphate (pH 3.5) and acetonitrile (58: 42, v/v) at a flow rate of 1ml/min. The system was found to produce sharp and well-resolved peak for atazanavir with retention time of 5.78 min. The linear regression analysis for the calibration curves showed a good linear correlation over the concentration range 0.050-2.0 μg/ml, with determination coefficients, R 2 , exceeding 0.9989. The limits of detection (LOD) and quantitation (LOQ) were found to be 0.004 μg/ml and 0.012 μg/ml, respectively. The method was successfully applied for the pharmacokinetic in rats. Atazanavir concentration in plasma reached (C max ) was 0.087 μg/ml at 3 h after oral administration of 7.2 mg/kg/rat. The AUC 0-12 was 0.4812 μg/ml*h and the apparent plasma half-life (t 1/2 ) was 7.5 h. This method was found to be suitable for examining atazanavir concentration in rats, after oral administration of atazanavir in a single dose.

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An isocratic liquid chromatography method for determining HIV non-nucleoside reverse transcriptase inhibitor and protease inhibitor concentrations in human plasma

Cited by 41 publications

References 12 publications

Spectrophotometric method for determination of atazanavir sulfate in capsule dosage form

Spectrophotometric method for determination of atazanavir sulfate in capsule dosage form

A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

Liquid Chromatographic Assay for the Analysis of Atazanavir in Rat Plasma after Oral Administration: Application to a Pharmacokinetic Study

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