Spectrophotometric method for determination of atazanavir sulfate in capsule dosage form

Behera, Anindita; Moitra, Swapan K.; Chandra, Sudam; Sankar, D. Gowri

doi:10.1590/s0100-40422011000800010

Cited by 3 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In method A, the drug was allowed to react with bromophenol blue and in method B, the drug was reacted with bromothymol blue in neutral medium. As compared to the reported spectrophotometric method by Behera et al 28 , the present methods are equally significant from the point of validation parameters and the present work demonstrates the simplified extraction free, ion complexation estimation by spectrophotometric method. The methods are easier than the conventional methods of ion complex estimation by extraction in suitable buffer system.…”

Section: Resultssupporting

confidence: 48%

See 1 more Smart Citation

Development and Statistical Correlation of Spectrophotometric Methods for Atazanavir Sulfate with Sulphonepthalein Dyes

Behera¹,

Sankar²,

Moitra³

et al. 2014

Asian J. Chem.

Self Cite

View full text Add to dashboard Cite

Atazanavir sulfate (ATV) (3S,8S,9S,12S)-3,12-bis(1,1dimethylethyl)-8-hydroxy-4,11-dioxo-9-(phenylmethyl)-6-[[4-(2-pyridinyl)phenyl]methyl]-2,5,6,10,13-pent-aazatetradecane dioic acid dimethyl ester (Fig. 1), an azapeptide is the 7 th protease inhibitor used in the treatment of human immunodeficiency virus (HIV) Type II infection 1. Atazanavir sulfate is official in IP 2010 2. Atazanavir sulfate is reported as poorly water soluble and a known substrate for both hepatic metabolizing enzyme cytochrome 450 (CYP3A) and intestinal drug efflux pump, P-glycoprotein (Pgp) so have low oral bioavailability 3. In literature several methods of analysis are reported for determination of atazanavir sulfate in blood plasma, biological cells and cerebrospinal fluid by HPLC 4-25. Stress degradation studies which were reported, analyzed by HPLC and ultraviolet-spectrophotometry 26,27. The authors have reported spectrophotometric methods 28 and HPLC method 29 in pharmaceutical dosage form. The present work is developed to simplify the extractive spectrophotometric methods using two sulphonepthalein dyes, i.e. brormophenol blue and bromothymol blue. EXPERIMENTAL Reference standard and raw material of atazanavir sulfate were procured from Matrix Laboratories (Hyderabad, India) as gift sample. Solvents used like chloroform, bromophenol

show abstract

Section: Resultssupporting

confidence: 48%

“…The ion pair complexes are easily extractable in chloroform, the method is simple. The methods are compared with the previously reported methods 28,29 and found to be equally significant. Statistical analysis: Two way ANOVA was applied to test both method-sample interaction and differences in method precision.…”

Section: Resultsmentioning

confidence: 99%

Development and Statistical Correlation of Spectrophotometric Methods for Atazanavir Sulfate with Sulphonepthalein Dyes

Behera¹,

Sankar²,

Moitra³

et al. 2014

Asian J. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

Simultaneous Determination of Emtricitabine and Tenofovir disoproxil fumarate in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography

Rele¹,

Patil²

2021

AJRC

View full text Add to dashboard Cite

A simple, rapid and accurate high performance liquid chromatography method is described for simultaneous determination of emtricitabine and tenofovir disoproxil fumarate from active pharmaceutical ingredients. The separation of drug was achieved on Hypersil BDS C18 (150 x 4.6 mm i.d.) with 5 µ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of a mixture of buffer and methanol (85:15 % v/v). The buffer was mixtures of 0.1 % (v/v) ortho-phosphoric acid solution adjusted the pH 3.5 with tri-ethyl amine. The detection was carried out at wavelength 260 nm. The mixture of buffer of pH 3.5 and methanol (85:15% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze emtricitabine and tenofovir disoproxil fumarate from active pharmaceutical ingredients.

show abstract

Critical Review of Chromatographic and Spectrophotometric Methods and Application of Uv- Auc Technique for Simple, Rapid, Quantitative Estimation of Atazanavir Sulfate

Bobade¹,

Ganorkar²

2017

IND. DRU.

View full text Add to dashboard Cite

Spectrophotometric analysis has proven to be one of the most referred analytical techniques available; as the enlarged globalization of the pharmaceutical market has reduced the availability of quality pharmaceuticals at minimal cost. Authors have made an attempt for development and validation of simple UV- spectrophotometric area under curve (UV -AUC) method for quantitative estimation of atazanavir sulfate in bulk and in a pharmaceutical formulation. To enhance accuracy, additional attempts were made for the measurement of uniform area around the wavelength maximum (248 nm) and in between the designated wavelengths (238 nm and 260 nm). The mixture of methanol and water in a proportion of 30:70 v/v was selected as a solvent system. Efficient correlation between the concentration and corresponding response was maintained by measuring the uniform area under the peak obtained at the wavelength maximum. The calibration plot for the method was constructed by plotting the values for AUC obtained for the concentration range of 6 - 36 μg/mL with a correlation coefficient of 0.999. Developed method was validated on the basis of ICH Q2 R1 guideline. The method depicted good reproducibility with percent RSD less than 2.0. Estimation of atazanavir sulfate by aforementioned method in bulk and capsules was carried out efficiently.

show abstract

Spectrophotometric method for determination of atazanavir sulfate in capsule dosage form

Cited by 3 publications

References 12 publications

Development and Statistical Correlation of Spectrophotometric Methods for Atazanavir Sulfate with Sulphonepthalein Dyes

Development and Statistical Correlation of Spectrophotometric Methods for Atazanavir Sulfate with Sulphonepthalein Dyes

Simultaneous Determination of Emtricitabine and Tenofovir disoproxil fumarate in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography

Critical Review of Chromatographic and Spectrophotometric Methods and Application of Uv- Auc Technique for Simple, Rapid, Quantitative Estimation of Atazanavir Sulfate

Contact Info

Product

Resources

About