A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

Yin, Kang; Meng, Xianmin; Dong, Pin; Ding, Tianran; Shen, Li; Zhang, Li; Zhang, Renfang; Cai, Weimin; Lu, Hongzhou

doi:10.5582/bst.2014.01002

Cited by 6 publications

(3 citation statements)

References 54 publications

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“…Efavirenz C 0 was determined by reverse-phase high-performance liquid chromatography with ultraviolet detection, according to our previously described method. 35 HPLC was performed using LC-20A (Shimadzu, Japan) consisting of a column compartment CTO-20A, degasser DGU-20A5, pump CBM-20A, auto-sampler SIL-20AC, and an SPD-20AV UV detector. The column was YMC-Pack ODS-A column (C18; 150 mm × 4.6 mm, 5 μm) with a pre-column (ZORBAX Eclipse Plus-C18).…”

Section: Methodsmentioning

confidence: 99%

Effects of rifampicin, CYP2B6 and ABCB1 polymorphisms on efavirenz plasma concentration in Chinese patients living with HIV and tuberculosis

et al. 2022

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Background Tuberculosis (TB) is the leading opportunistic infection of people living with human immunodeficiency virus (HIV; PLWH). Cytochrome P450 (CYP) 2B6 and ATP-binding cassette sub-family B member 1 ( ABCB1) are involved in the metabolism and transportation of efavirenz. The study was aimed to investigate the effects of rifampicin, CYP2B6 and ABCB1 polymorphisms on efavirenz exposure in Chinese PLWH co-infected with TB. Method PLWH were screened according to inclusion and exclusion criteria and divided into HIV group and HIV/TB group. Efavirenz plasma concentration (C0) was determined, dose-adjusted concentration (C0/D) was calculated, and genotypes of CYP2B6 516G>T, 785A>G, and ABCB1 2677G>T, 3435C>T were analyzed. Results 252 PLWH were enrolled, including 75 co-infected with TB and concomitant with rifampicin. Efavirenz C0 and C0/D were both higher in HIV group (1.94 μg/mL, 0.2007 (μg/ml)/(mg/kg/d)) compared with HIV/TB group (1.52 μg/mL, 0.1557 (μg/ml)/(mg/kg/d)) ( p = .001). Efavirenz C0/D was significantly higher in patients with variant genotypes of CYP2B6 516G>T and 785A>G ( p<.001), and was significantly lower in HIV/TB group compared with HIV group among patients with CYP2B6 516 GG, TT, and 785 AA, AG genotypes ( p < .05). Conclusion Efavirenz exposure is reduced by co-administration with rifampicin, and related to genetic polymorphisms of CYP2B6.

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Section: Methodsmentioning

confidence: 99%

Effects of rifampicin, CYP2B6 and ABCB1 polymorphisms on efavirenz plasma concentration in Chinese patients living with HIV and tuberculosis

et al. 2022

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“…The HPLC (Shimadzu, Kyoto, Japan) system consists of a binary pump (LC-20AD), equipped with UV/Visible detector (SPD-A20), a Rheodyne injector port (7725i) with 20 μl loop volume and the data [19,20]. To 200 µl rabbit plasma (blank/test/CS/QC) sample transferred in an eppendorf tube (2 ml) quadruple volume (800 ml) of ACN and 20 µl WS solution of RAB (5 µg/ml) was added as extraction reagent and IS respectively, capped and vortexed on a cyclomixture for 1 min.…”

Section: Instrumentation and Chromatographic Conditionmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Estimation of Repaglinide in Rabbit Plasma

Navamanisubramanian¹,

Panchagiri

Nerella³

et al. 2019

Int J App Pharm

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Objective: A simple, selective and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method to estimate repaglinide (REP) in rabbit plasma using rabeprazole (RAB) as an internal standard was developed and validated for various qualifications. Methods: The chromatographic separation was performed on C18 (2) analytical column (5 μ, 250×4.6 mm) using acetonitrile: 0.05% trifluoroacetic acid in water (55:45, v/v) as mobile phase at the flow rate of 1 ml/min. Validation of the analytical method was performed as per ICH guidelines. Results: The retention times of REP and RAB were found at ~4.3 and 5.1 min respectively, with adequate system suitability parameters (theoretical plates ≥3619, tailing factor ≤1.38, resolution factor 2.37). The method has linearity over a concentration range of 10 to 1000 ng/ml (r2=0.9987). The results of accuracy (≥98.17%), intra-, inter-day precision (≤2.9%), recovery (101.21±2.09%) and process efficiency (99.77±3.74%) found satisfactory with no matrix effect. The analyte in samples were found stable up to 6 h, 3 freeze-thaw cycles and not more than 2 mo corresponding to bench-top, short and long term stability studies respectively. Conclusion: The developed RP-HPLC method for estimation of REP in rabbit plasma was developed. The method was found to be rapid, cost-effective and accurate to estimate the REP from the sample matrix. The method can be a most useful tool for in vivo study of REP in the rabbit.

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“…During the research and development of anticancer agent GLB, the pharmacokinetic profile is needed for understanding the absorption, distribution, metabolism and excretion (ADME) of GLB in vivo 22 . The high performance liquid chromatographic - ultraviolet detector (HPLC-UV) is a conventional quantitative analysis method 23 24 25 26 . In according to the ultraviolet absorption wavelength, elution retention time and chromatographic peak area, the content of compound can be accurately measured 27 28 29 30 .…”

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confidence: 99%

Quantitative and qualitative analysis of the novel antitumor 1,3,4-oxadiazole derivative (GLB) and its metabolites using HPLC-UV and UPLC-QTOF-MS

Wang

Li³

et al. 2015

Sci Rep

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Fructose-based 3-acetyl-2,3-dihydro-1,3,4-oxadiazole (GLB) is a novel antitumor agent and belongs to glycosylated spiro-heterocyclic oxadiazole scaffold derivative. This research first reported a simple, specific, sensitive and stable high performance liquid chromatography -ultraviolet detector (HPLC-UV) method for the quantitative determination of GLB in plasma. In this method, the chromatographic separation was achieved with a reversed phase C18 column. The calibration curve for GLB was linear at 300 nm. The lower limit of quantification was 10 ng/mL. The precision, accuracy and stability of the method were validated adequately. This method was successfully applied to the pharmacokinetic study in rats for detection of GLB after oral administration. Moreover, the structures of parent compound GLB and its two major metabolites M1 and M2 were identified in plasma using an ultra performance liquid chromatography- electrospray ionization-quadrupole-time of flight- mass spectrometry (UPLC-ESI-QTOF-MS) method. Our results indicated that the di-hydroxylation (M1) and hydroxylation (M2) of GLB are the major metabolites. In conclusion, the present study provided valuable information on an analytical method for the determination of GLB and its metabolites in rats, can be used to support further developing of this antitumor agent.

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A simple, rapid, economical, and practical method for the determination of efavirenz in plasma of Chinese AIDS patients by reverse phase high-performance liquid chromatography with ultraviolet detector

Cited by 6 publications

References 54 publications

Effects of rifampicin, CYP2B6 and ABCB1 polymorphisms on efavirenz plasma concentration in Chinese patients living with HIV and tuberculosis

Effects of rifampicin, CYP2B6 and ABCB1 polymorphisms on efavirenz plasma concentration in Chinese patients living with HIV and tuberculosis

Stability Indicating Rp-HPLC Method for Estimation of Repaglinide in Rabbit Plasma

Quantitative and qualitative analysis of the novel antitumor 1,3,4-oxadiazole derivative (GLB) and its metabolites using HPLC-UV and UPLC-QTOF-MS

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