AN INVESTIGATION OF A COLLECTION OF YELLOW‐PIGMENTED <i>PSEUDOMONAS</i>

Søgaard, Per; Hansen, Bente Gahrn; Frederiksen, Wilhelm

doi:10.1111/j.1699-0463.1986.tb03034.x

Cited by 2 publications

(3 citation statements)

References 19 publications

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“…A comparison between Table 2 in this paper and Table 3 in ref. 6 shows that the diagnoses of API were not improbable. Strain 13 was found p-galactosidase negative and ESC-positive in both the API and the conventional system.…”

Section: Discussionmentioning

confidence: 97%

“…Thirty-six strains of yellow-pigmented Pseudomonas from clinical as well as non-clinical material and I I local reference strains of Pseudomonas were studied. The 36 yellow-pigmented strains have been identified by means of conventional methods and the results presented elsewhere (6). Eighteen of the strains were classified as P. paucimobilis, eight strains as CDC group VE-I and 2, one strain as P. vesicularis and one as P. putida.…”

Section: Strainsmentioning

confidence: 99%

“…Eight strains of yellow-pigmented P.scwlomona.s remained unidentified by the conventional method (6). An attempt was made to identify these strains with the three commercial systems.…”

Section: P Pseudomallei Was Not Listed In the Roscomentioning

confidence: 99%

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An Investigation of Three Commercial Methods for Rapid Identification of Non‐enteric Gram‐negative Rods

Søgaard

Gahrn-Hansen

HuiPing

et al. 1986

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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Three commercial systems for the identification of non‐enteric gram‐negative rods were compared with conventional bacteriological methods as reference. The three systems were the API 20 NE, BIO‐TEST ID‐Trident, and ROSCO Diagnostic Tablets. The systems were tested on a set of 47 strains from the genus Pseudomonas, with the emphasis upon the yellow‐pigmented species. The overall identification accuracy was 97% with the API, 19% with the BIOTEST and 68% with the ROSCO system. The API system was thus reliable, and it presented no major practical problems. The BIOTEST system was very handy. The main reason for the low accuracy was an error in the code book. The ONPG reaction was depicted as negative in the species P. paucimobilis. A positive ONPG test is a keymark in this species and 76% of the 20 strains of P. paucimobilis included in this investigation were also found positive with the BIOTEST system. The ROSCO tablets are convenient if the result of single reactions is desired. The time consumption per strain was 5.4, 4.4, and 6.2 min for the API, BIOTEST, and ROSCO systems respectively.

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Section: Discussionmentioning

confidence: 97%

Section: Strainsmentioning

confidence: 99%