An integrated microfluidic electrochemical assay for cervical cancer detection at point-of-care testing

Abstract

IMEAC is an integrated microfluidic electrochemical assay for cervical cancer detection at point of-care testing.

A microfluidic plasma separation device combined with a surface plasmon resonance biosensor for biomarker detection in whole blood

“…The PPS-V2 device can be integrated with other diagnostic assays for various applications in POC testing. 32 The integration with an SPR biosensor can be readily applied to real-time, label free, and continuous detection of clinically important biomarkers, including in bacterial and viral diseases (e.g., hepatitis, influenza, AIDS, HIV, and SARS-Cov2) from low volumes of whole blood samples. The primary advantages of our assay are the use of whole blood for biomarker detection without the need for sample pre-processing; it is easy to fabricate, portable, and disposable; it uses a low reagent sample volume.…”

“…The PPS-V2 device was fabricated with PDMS using conventional microfabrication following standard photolithography techniques. 32 The device integrates three PDMS layers from bottom to top with microcapillaries and a 9 μm thick membrane in between the middle layer and the top layer (Fig. 1a).…”

“…26 A 9 μm thick filter membrane with an average pore size of 0.6 μm was cut into small rectangular pieces (7 mm × 5 mm) and bonded between the middle layer (PDMS layer 2) and the top layer (PDMS layer 3) using plasma bonding (Fig. 1c) 32 to improve blood cell depletion. APTES was used to strengthen the bonding at membrane-PDMS interfaces before plasma bonding.…”

“…4c). The purity of the filtered plasma was calculated using flow cytometry 32 and was assessed by counting the number of blood cells in the inlet blood and outlet plasma (Fig. S1 †).…”

“…The yield was obtained by dividing the collected plasma volume using our system by the plasma volume under ideal conditions (centrifugation). 32 We achieved a purity of ∼86% and a yield of ∼25% using the PPS-V2 device (more information in the ESI †).…”