Affinity-based electrochemical sensors for biomolecular detection in whole blood

Wilkirson, Elizabeth C; Singampalli, Kavya L; Li, Jiran; Dixit, Desh Deepak; Jiang, Xue; Gonzalez, Diego H; Lillehoj, Peter B.

doi:10.1007/s00216-023-04627-5

Cited by 16 publications

(10 citation statements)

References 90 publications

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“…Affinity recognition is indispensable for the detection of disease biomarkers, including proteins and nucleic acids, with most biosensors adopting this approach falling under the category of voltammetric biosensors [ 93 , 94 ]. Since these sensors require electroactive labeling for target detection, they can be fabricated by immobilizing a capture biomolecule (e.g., DNA, peptides, antibodies, aptamers) on a working electrode to detect proteins or nucleic acids using a sandwich assay format, and self-assembling molecules described in the previous section can be used.…”

Section: Electrochemical Biosensing Platformsmentioning

confidence: 99%

Self-assembling biomolecules for biosensor applications

Kim,

Kang,

Kwon

et al. 2023

Biomater Res

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Molecular self-assembly has received considerable attention in biomedical fields as a simple and effective method for developing biomolecular nanostructures. Self-assembled nanostructures can exhibit high binding affinity and selectivity by displaying multiple ligands/receptors on their surface. In addition, the use of supramolecular structure change upon binding is an intriguing approach to generate binding signal. Therefore, many self-assembled nanostructure-based biosensors have been developed over the past decades, using various biomolecules (e.g., peptides, DNA, RNA, lipids) and their combinations with non-biological substances. In this review, we provide an overview of recent developments in the design and fabrication of self-assembling biomolecules for biosensing. Furthermore, we discuss representative electrochemical biosensing platforms which convert the biochemical reactions of those biomolecules into electrical signals (e.g., voltage, ampere, potential difference, impedance) to contribute to detect targets. This paper also highlights the successful outcomes of self-assembling biomolecules in biosensor applications and discusses the challenges that this promising technology needs to overcome for more widespread use. Graphical Abstract

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Section: Electrochemical Biosensing Platformsmentioning

confidence: 99%

Self-assembling biomolecules for biosensor applications

Kim,

Kang,

Kwon

et al. 2023

Biomater Res

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“…Long-term stability of sensors in these samples needs study on materials and designs ensuring sustained performance. Additionally, understanding and mitigating interference from various substances in plasma and serum constitute key research areas for improving drug detection specificity [3,16].…”

Section: Blood and Derivativesmentioning

confidence: 99%

Sensors in the Detection of Abused Substances in Forensic Contexts: A Comprehensive Review

Rosendo,

Antunes,

Simão

et al. 2023

Micromachines

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Forensic toxicology plays a pivotal role in elucidating the presence of drugs of abuse in both biological and solid samples, thereby aiding criminal investigations and public health initiatives. This review article explores the significance of sensor technologies in this field, focusing on diverse applications and their impact on the determination of drug abuse markers. This manuscript intends to review the transformative role of portable sensor technologies in detecting drugs of abuse in various samples. They offer precise, efficient, and real-time detection capabilities in both biological samples and solid substances. These sensors have become indispensable tools, with particular applications in various scenarios, including traffic stops, crime scenes, and workplace drug testing. The integration of portable sensor technologies in forensic toxicology is a remarkable advancement in the field. It has not only improved the speed and accuracy of drug abuse detection but has also extended the reach of forensic toxicology, making it more accessible and versatile. These advancements continue to shape forensic toxicology, ensuring swift, precise, and reliable results in criminal investigations and public health endeavours.

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“…The electrochemical detection test’s selectivity and sensitivity can be significantly enhanced by optimizing the electrode’s design and surface functionalization. This enables the accurate measurement of low-abundance biomarkers in complex biological samples . In the following sections, we discuss how 3D printing techniques have been employed to fabricate implantable microelectrodes for the electrochemical detection of biomarkers, addressing critical design considerations and exploring their potential applications in diagnosing cardiovascular and neurodegenerative diseases (Figure ).…”

Section: Electrochemical Detection Of Biomarkers For the Early Diagno...mentioning

confidence: 99%

Use of 3D Printing Techniques to Fabricate Implantable Microelectrodes for Electrochemical Detection of Biomarkers in the Early Diagnosis of Cardiovascular and Neurodegenerative Diseases

Zilinskaite,

Shukla,

Baradoke

2023

ACS Meas. Sci. Au

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This Review provides a comprehensive overview of 3D printing techniques to fabricate implantable microelectrodes for the electrochemical detection of biomarkers in the early diagnosis of cardiovascular and neurodegenerative diseases. Early diagnosis of these diseases is crucial to improving patient outcomes and reducing healthcare systems' burden. Biomarkers serve as measurable indicators of these diseases, and implantable microelectrodes offer a promising tool for their electrochemical detection. Here, we discuss various 3D printing techniques, including stereolithography (SLA), digital light processing (DLP), fused deposition modeling (FDM), selective laser sintering (SLS), and two-photon polymerization (2PP), highlighting their advantages and limitations in microelectrode fabrication. We also explore the materials used in constructing implantable microelectrodes, emphasizing their biocompatibility and biodegradation properties. The principles of electrochemical detection and the types of sensors utilized are examined, with a focus on their applications in detecting biomarkers for cardiovascular and neurodegenerative diseases. Finally, we address the current challenges and future perspectives in the field of 3D-printed implantable microelectrodes, emphasizing their potential for improving early diagnosis and personalized treatment strategies.

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Affinity-based electrochemical sensors for biomolecular detection in whole blood

Cited by 16 publications

References 90 publications

Self-assembling biomolecules for biosensor applications

Self-assembling biomolecules for biosensor applications

Sensors in the Detection of Abused Substances in Forensic Contexts: A Comprehensive Review

Use of 3D Printing Techniques to Fabricate Implantable Microelectrodes for Electrochemical Detection of Biomarkers in the Early Diagnosis of Cardiovascular and Neurodegenerative Diseases

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