A microfluidic plasma separation device combined with a surface plasmon resonance biosensor for biomarker detection in whole blood

Abstract

Biomarker detection in whole blood enables understanding of the cause, progression, relapse, or outcome of treatment of a disease. Conventional biomarker detection techniques such as enzyme-linked immunosorbent assay, polymerase chain...

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

“…Similarly, Debnath et al connected a blood‐plasma‐separation chip to a commercial SPR sensor chip via microfluidic tubing, which eliminated the need for complex sensor surface preparation steps. [ 148 ] Although such plug‐and‐play configurations are not by definition one integrated system, they might allow for integrated workflows performed in overall integrated platforms. In one of the examples, we have discussed in Section 4.1.2, enzymatic secretion of leukocytes found in the whole blood samples of patients suffering from acute heart failure were profiled, revealing patient‐specific immune responses.…”

“…One way to overcome this limitation was recently demonstrated by Debnath et al where a plug‐and‐play system configuration was used to connect a blood‐plasma‐separation chip to a commercial SPR sensor chip via microfluidic tubing. [ 148 ] This system combination allowed for the processing of whole blood samples in the separation chip and the detection of IgG and IgM antibodies in the SPR sensor chip in less than 15 min. Another system reported by Xiao et al used 3D‐printing and adhesive tape‐based sealing to realize a microfluidic platform that could be directly placed on top of the sensing layer of a commercial SPR sensor.…”

“…One way to overcome such challenges, especially when required methods or processes are in some way incompatible, is to further adopt plug-and-play configurations, as discussed in two examples earlier. [69,148] Briefly, Antfolk et al bonded an acoustophoretic chip facilitating rare cell pre-concentration and a DEP chip to trap rare cells in an array of electroactive microwells for further analysis. [69] The use of separate but connected chips allowed the authors to overcome limitations regarding the integration of bulk piezoelectric transducers into the microsystem.…”

High-Throughput Microfluidic Extraction of Platelet-free Plasma for MicroRNA and Extracellular Vesicle Analysis

“…Microfluidics with the ability to precisely manipulate micro/nanoparticles have been widely applied for plasma isolation using principles of filtration, , deterministic lateral displacement, acoustics, and dielectrophoresis, but these methods suffer from limited throughput or yield. Centrifugal microfluidics , provides an alternative with higher throughput, but its limited capacity and complex fabrication affect its adoption.…”

“…As plasma contaminants like ex vivo released cellular EVs share similar origin with circulating EVs, it is challenging to separate them using downstream EV isolation or detection which may cause high background in EV analysis. Microfluidics with the ability to precisely manipulate micro/ nanoparticles have been widely applied for plasma isolation using principles of filtration, 21,22 deterministic lateral displacement, 23 acoustics, 24 and dielectrophoresis, 25 but these methods suffer from limited throughput or yield. Centrifugal microfluidics 26,27 provides an alternative with higher throughput, but its limited capacity and complex fabrication affect its adoption.…”