An integrated metabolism in vivo analysis and network pharmacology in UC rats reveal anti-ulcerative colitis effects from Sophora flavescens EtOAc extract

Chen, Lei; Shao, Jing; Luo, Yun; Zhao, Linlin; Zhao, Kairui; Gao, Yanping; Wang, Shumei; Liu, Yi

doi:10.1016/j.jpba.2020.113306

Cited by 19 publications

(20 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the KEGG network pharmacology analysis, gastritis-related genes were associated with nine pathways. Among them, the PI3K-Akt signaling pathway and NF-kB-mediated signaling pathway were closely correlated with the pathogenesis of gastric disease and intestinal mucosal injury [51]. A. rugosa extract effectively reduced NO and NF-κB expression compared with LPS-treated control (Figure 3).…”

Section: Discussionmentioning

confidence: 94%

Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

et al. 2020

View full text Add to dashboard Cite

Agastache rugosa is used as a Korean traditional medicine to treat gastric diseases. However, the active ingredients and pharmacological targets of A. rugosa are unknown. In this study, we aimed to reveal the pharmacological effects of A. rugosa on gastritis by combining a mice model and a network pharmacology method. The macrophage and gastritis-induced models were used to evaluate the pharmacological effects of A. rugosa. The results show that A. rugosa relieved mucosal damage induced by HCl/EtOH in vivo. Network analysis identified 99 components in A. rugosa; six components were selected through systematic screening, and five components were linked to 45 gastritis-related genes. The main components were acacetin and luteolin, and the identified core genes were AKT serine/threonine kinase 1 (AKT1), nuclear factor kappa B inhibitor alpha (NFKBIA), and mitogen-activated protein kinase-3 (MAPK3) etc. in this network. The network of components, target genes, protein–protein interactions, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was closely connected with chemokines and with phosphoinositide 3-kinase-Akt (PI3K/AKT), tumor-necrosis-factor alpha (TNFα), mitogen-activated protein kinase, nuclear factor kappa B, and Toll-like receptor (TLR) pathways. In conclusion, A. rugosa exerts gastro-protective effects through a multi-compound and multi-pathway regulatory network and holds potential for treating inflammatory gastric diseases.

show abstract

Section: Discussionmentioning

confidence: 94%

Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Based on the traditional Chinese medicine (TCM) theory, UC consists of several types of TCM syndromes, the most common of which (34.8%) being damp-heat accumulation syndrome (DHAS) ( Ye et al, 2010 ). Radix Sophorae flavescentis (Kushen) is a monarch herb in TCM formulations (Kushen-based TCM formulations) used for the treatment of UC with DHAS which has achieved good effectiveness in clinical practice and effect studies ( Tong et al, 2010 ; Tong et al, 2011 ; Gong et al, 2012 ; Fang et al, 2018 ; Chen et al, 2020 ). Kushen mainly contains the components matrine and oxymatrine , which are active ingredients against UC with significant inhibitory effects on invasive microbial populations such as Escherichia coli and Proteus mirabilis, as well as immunosuppressive effects against IL-1β and IL-17 in the intestinal epithelium ( Lin et al, 2019 ; Xu et al, 2019 ; Ding et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Efficacy and Safety of Sophora flavescens (Kushen) Based Traditional Chinese Medicine in the Treatment of Ulcerative Colitis: Clinical Evidence and Potential Mechanisms

2020

View full text Add to dashboard Cite

Background: Radix Sophorae flavescentis (Kushen), a Chinese herb, is widely used in the treatment of ulcerative colitis (UC) with damp-heat accumulation syndrome (DHAS) according to traditional Chinese medicine (TCM) theory.Objective: The aim of this study was to illuminate the clinical efficacy and potential mechanisms of Kushen-based TCM formulations in the treatment of UC with DHAS.Materials and Methods: A systematic literature search was performed in the PubMed, EMBASE, Chinese Biomedical Literature database, China National Knowledge Infrastructure database, Chongqing VIP Information database, and Wanfang database for articles published between January 2000 and July 2020 on randomized controlled trials (RCTs) that used Kushen-based TCM formulations in the treatment of UC with DHAS. A network pharmacology approach was conducted to detect the potential pathways of Kushen against UC with DHAS.Results: Eight RCTs with a total of 983 subjects were included in the meta-analysis. Compared with the control subjects (5-aminosalicylic acid therapy), those who received Kushen-based TCM formulations for the treatment of UC showed a significantly higher clinical remission rate (RR = 1.20, 95% CI: [1.04, 1.38], p = 0.02) and lower incidence of adverse events (RR = 0.63, 95% CI [0.39, 1.01], p = 0.06). A component-target-pathway network was constructed, indicating five main components (quercetin, luteolin, matrine, formononetin, and phaseolin), three major targets (Interleukin-6, Myc proto-oncogene protein, and G1/S-specific cyclin-D1) and one key potential therapeutic pathway (PI3K-Akt signaling) of Kushen against UC with DHAS.Conclusion: Kushen-based TCM formulations provide good efficacy and possess great potential in the treatment of UC. Large-scale and high-quality clinical trials and experimental verification should be considered for further confirmation of the efficacy of Kushen-based formulations.

show abstract

“…Additionally, network pharmacology was used to determine which genes correlated with Alzheimer’s disease, for the purpose of finding potential signaling pathways and novel compounds ( Yi et al, 2020 ). Network pharmacology analysis was also used to find various active compounds for treating ulcerative colitis that contained formononetin, kushenol N, and kuraridin, to lay the foundation for studying the disease ( Chen et al, 2020 ). Overall, the study of network pharmacology can preliminarily predict the component monomers and disease targets of TCM to lay a foundation for elucidating the therapeutic mechanism of TCM, and it also can be applied in the study of EAE.…”

Section: Novel Strategies Used For Developing New Natural Productsmentioning

confidence: 99%

“…There have been many studies of the treatment of EAE by TCM monomers containing flavonoids, phenol, and glycosides, among others ( Ciumărnean et al, 2020 ; Ikram et al, 2020 ; Yang L. et al, 2020 ). Moreover, many researchers have studied the effect of chemical monomers by molecular docking, high-throughput screening, and network pharmacology ( Chen et al, 2020 ; Liu J. et al, 2020 ; Xu et al, 2020a ). Over the past 8 years, glycosides have been the most commonly studied compounds, and have been examined and developed with the intent of using them as a potential treatment for EAE ( Giacoppo et al, 2013 ; Yin et al, 2014 ; Haghmorad et al, 2017 ; Madhu et al, 2019 ; Yang L. et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Role of Plant-Derived Natural Compounds in Experimental Autoimmune Encephalomyelitis: A Review of the Treatment Potential and Development Strategy

et al. 2021

View full text Add to dashboard Cite

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system that is mainly mediated by pathological T-cells. Experimental autoimmune encephalomyelitis (EAE) is a well-known animal model of MS that is used to study the underlying mechanism and offers a theoretical basis for developing a novel therapy for MS. Good therapeutic effects have been observed after the administration of natural compounds and their derivatives as treatments for EAE. However, there has been a severe lag in the research and development of drug mechanisms related to MS. This review examines natural products that have the potential to effectively treat MS. The relevant data were consulted in order to elucidate the regulated mechanisms acting upon EAE by the flavonoids, glycosides, and triterpenoids derived from natural products. In addition, novel technologies such as network pharmacology, molecular docking, and high-throughput screening have been gradually applied in natural product development. The information provided herein can help improve targeting and timeliness for determining the specific mechanisms involved in natural medicine treatment and lay a foundation for further study.

show abstract

An integrated metabolism in vivo analysis and network pharmacology in UC rats reveal anti-ulcerative colitis effects from Sophora flavescens EtOAc extract

Cited by 19 publications

References 51 publications

Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

Efficacy and Safety of Sophora flavescens (Kushen) Based Traditional Chinese Medicine in the Treatment of Ulcerative Colitis: Clinical Evidence and Potential Mechanisms

Role of Plant-Derived Natural Compounds in Experimental Autoimmune Encephalomyelitis: A Review of the Treatment Potential and Development Strategy

Contact Info

Product

Resources

About