Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

Nam, Hyeon‐Hwa; Kim, Joong Sun; Lee, Jun; Seo, Young Hye; Kim, Hyo Seon; Ryu, Seung Mok; Choi, Goya; Moon, Byeong Cheol; Lee, A Yeong

doi:10.3390/biom10091298

Cited by 25 publications

(25 citation statements)

References 59 publications

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“…The identification of gene networks controlled by ingredients is one way to garner information about how ingredients exhibit their specific therapeutic efficacy because a characteristic of medicinal plants is that they are the multi-ingredient, multi-targets, and multi-biological activities [ 14 , 15 ]. A good explanation for this role is the network pharmacology approach by Hopkins in 2008 [ 16 ]. Considering previous studies, we hypothesized that L. tanakae would have the potential to treat COPD.…”

Section: Introductionmentioning

confidence: 99%

Loranthus tanakae Franch. & Sav. Suppresses Inflammatory Response in Cigarette Smoke Condensate Exposed Bronchial Epithelial Cells and Mice

et al. 2022

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Loranthus tanakae Franch. & Sav. found in China, Japan, and Korea is traditionally used for managing arthritis and respiratory diseases. In this study, we analyzed the components of L. tanakae 70% ethanol extract (LTE) and investigated the therapeutic effects of LTE on pulmonary inflammation using cells exposed to cigarette smoke condensate (CSC) and lipopolysaccharide (LPS) in vitro and in vivo in mice and performed a network analysis between components and genes based on a public database. We detected quercitrin, afzelin, rhamnetin 3-rhamnoside, and rhamnocitrin 3-rhamnoside in LTE, which induced a significant reduction in inflammatory mediators including interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and inflammatory cells in CSC exposed H292 cells and in mice, accompanied by a reduction in inflammatory cell infiltration into lung tissue. In addition, LTE increased translocation into the nuclei of nuclear factor erythroid-2-related factor 2 (Nrf2). By contrast, the activation of nuclear factor (NF)-κB, induced by CSC exposure, decreased after LTE application. These results were consistent with the network pharmacological analysis. In conclusion, LTE effectively attenuated pulmonary inflammation caused by CSC+LPS exposure, which was closely involved in the enhancement of Nrf2 expression and suppression of NF-κB activation. Therefore, LTE may be a potential treatment option for pulmonary inflammatory diseases including chronic obstructive pulmonary disease (COPD).

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Section: Introductionmentioning

confidence: 99%

Loranthus tanakae Franch. & Sav. Suppresses Inflammatory Response in Cigarette Smoke Condensate Exposed Bronchial Epithelial Cells and Mice

et al. 2022

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“…KEGG pathway enrichment analysis displayed that 60 common targets were markedly enriched in Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, apoptosis, T cell receptor signaling pathway, MAPK signaling pathway, cytokine-cytokine receptor interaction pathway, and chemokine signaling pathway. Toll-like receptor signaling pathway, MAPK signaling pathway, and chemokine signaling pathway are the main pathways associated with Agastache rugosa treatment of gastritis [ 27 ]. The ethyl acetate extract of Alpinia officinarum Hance inhibits the development of Helicobacter pylori-associated gastritis by regulating MAPK signaling pathway [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…with Agastache rugosa treatment of gastritis [27]. The ethyl acetate extract of Alpinia officinarum Hance inhibits the development of Helicobacter pylori-associated gastritis by regulating MAPK signaling pathway [28].…”

Section: Gene Expressionmentioning

confidence: 99%

Uncovering the Mechanisms and Molecular Targets of Weibing Formula 1 against Gastritis: Coupling Network Pharmacology with GEO Database

Chen¹,

Zhang

Zhou

et al. 2021

BioMed Research International

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Weibing Formula 1, a classic traditional formula, has been widely used clinically to treat gastritis in recent years. However, the potential pharmacological mechanism of Weibing Formula 1 is still unclear to date. A network pharmacology-based strategy was performed to uncover the underlying mechanisms of Weibing Formula 1 against gastritis. Furthermore, we structured the drug-active ingredients-genes–disease network and PPI network of shared targets, and function enrichment analysis of these targets was carried out. Ultimately, Gene Expression Omnibus (GEO) datasets and real-time quantitative PCR were used to verify the related genes. We found 251 potential targets corresponding to 135 bioactive components of Weibing Formula 1. Then, 327 gastritis-related targets were known gastritis-related targets. Among which, 60 common targets were shared between potential targets of Weibing Formula 1 and known gastritis-related targets. The results of pathway enrichment analysis displayed that 60 common targets mostly participated in various pathways related to Toll-like receptor signaling pathway, MAPK signaling pathway, cytokine-cytokine receptor interaction pathway, chemokine signaling pathway, and apoptosis. Based on the GSE60427 dataset, 15 common genes were shared between differentially expressed genes and 60 candidate targets. The verification results of the GSE5081 dataset showed that except for DUOX2 and VCAM1, the other 13 genes were significantly upregulated in gastritis, which was consistent with the results in the GSE60427 dataset. More importantly, real-time quantitative PCR results showed that the expressions of PTGS2, MMP9, CXCL2, and CXCL8 were significantly upregulated and NOS2, EGFR, and IL-10 were downregulated in gastritis patients, while the expressions of PTGS2, MMP9, CXCL2, and CXCL8 were significantly downregulated and NOS2, EGFR, and IL-10 were upregulated after the treatment of Weibing Formula 1. PTGS2, NOS2, EGFR, MMP9, CXCL2, CXCL8, and IL-10 may be the important direct targets of Weibing Formula 1 in gastritis treatment. Our study revealed the mechanism of Weibing Formula 1 in gastritis from an overall and systematic perspective, providing a theoretical basis for further knowing and application of this formula in the future.

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“…Although our top-four ligands generally interact with cellular enzymes, lyases, proteases and kinases according to SwissTarget analysis (Figures 7A, 7B, 7C and 7D), based on probability analysis, only rosmarinic acid showed potential for interaction (p = 0.96) with its putative targets (lyases, various enzymes, proteases and kinases). Interestingly, it should be noted that rosmarinic acid showed an anti-inflammatory effect by inhibiting the PI3K-Akt pathway stimulated by IL2 in mice gastric cells (Nam et al, 2020) and by inhibiting the kinase IKK-β (inhibitor of nuclear factor kappa-B kinase subunit beta) activity in human dermal fibroblasts (Lee et al, 2006). This reported that inhibitory potential of rosmarinic acid on interleukins and kinases in the inflammatory pathway is in high agreement with the 'targets-components analysis' we performed on the STITCH public server.…”

Section: Discussionmentioning

confidence: 99%

Molecular interactions of some phenolics with 2019-nCoV and related pathway elements

İstifli

Tepe

Sarıkürkçü

et al. 2021

International Journal of Secondary Metabolite

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As of June 2021, the novel coronavirus disease (SARS-CoV-2) resulted in 180 million cases worldwide and resulted in the death of approximately 4 million people. However, an effective pharmaceutical with low side effects that can be used in the treatment of SARS-CoV-2 infection has not been developed yet. The aim of this computational study was to analyze the interactions of twenty-two hydroxycinnamic acid and hydroxybenzoic acid derivatives with the SARS-CoV-2 receptor binding domain (RBD) and host organism's proteases, transmembrane serine protease 2 (TMPRSS2), and cathepsin B and L (CatB/L). According to the RBCI analysis, the ligands with the highest affinity against 4 enzymes in the molecular docking study were determined as 1-caffeoyl-β-D-glucose, rosmarinic acid, 3-p-coumaroylquinic acid and chlorogenic acid. It has also been observed that these compounds interacted more strongly with spike RBD, CatB and CatL enzymes. Although the top-ranked ligand, 1-caffeoyl-β-D-glucose, violated the drug-likeness criteria at 1 point (NH or OH>5) and ADMET in terms of AMES toxicity, the second top-ranked ligand rosmarinic acid neither violated drug-likeness nor exhibited incompatibility in terms of ADMET. In conclusion, with its antiinflammatory properties, rosmarinic acid can be considered and further investigated as a plant-based pharmaceutical that can offer a treatment option in SARS-CoV-2 infection. However, our findings should be supported by additional in vitro and in vivo studies.

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Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

Cited by 25 publications

References 59 publications

Loranthus tanakae Franch. & Sav. Suppresses Inflammatory Response in Cigarette Smoke Condensate Exposed Bronchial Epithelial Cells and Mice

Loranthus tanakae Franch. & Sav. Suppresses Inflammatory Response in Cigarette Smoke Condensate Exposed Bronchial Epithelial Cells and Mice

Uncovering the Mechanisms and Molecular Targets of Weibing Formula 1 against Gastritis: Coupling Network Pharmacology with GEO Database

Molecular interactions of some phenolics with 2019-nCoV and related pathway elements

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