An injectable chitosan/dextran/β -glycerophosphate hydrogel as cell delivery carrier for therapy of myocardial infarction

Xiao, Ke; Li, Mingyan; Wang, Xiaoqing; Liang, J. Nellie; Wang, Xing; Wu, Shaoyun; Long, Ming; Hu, Chengping

doi:10.1016/j.carbpol.2019.115516

Cited by 87 publications

(49 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrogel solutions could all be easily injected into a PBS solution at 37 • C. A sol-gel transition was observed as the temperature increased, showing the hydrogel thermo-responsiveness (see Videos S1, S2, S3 in SI). The inversion tube test results, in agreement with literature, indicated that the gel state of CH-SHC systems is reached after a few minutes of incubation at 37 • C [43]. The addition of an organic salt, BGP, and L-Arg, did not induce any change in the thermoresponsive behavior of the CH+SHC system.…”

Section: Discussionsupporting

confidence: 89%

“…Conversely, the presence of ARG does not change the hydrogel mechanical properties in compression mode even if a stabilizing effect has been underlined by swelling test. Interestingly, the three hydrogel formulations provide different Young values in compression at low strain values according to the GA composition but always in the range of most of "soft" biological tissues, i.e., 1-10 KPa [41][42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation

et al. 2020

View full text Add to dashboard Cite

The three-dimensional complexity of the native extracellular matrix (ECM) suggests switching from 2D to 3D culture systems for providing the cells with an architecture more similar to the physiological environment. Reproducing the three-dimensionality in vitro can guarantee beneficial effects in terms of cell growth, adhesion, proliferation, and/or their differentiation. Hydrogels have the same tailorable physico-chemical and biological characteristics as ECM materials. In this study, we propose a thermoresponsive chitosan-based hydrogel that gels thanks to the addition of organic and inorganic salt solutions (beta-glycerolphosphate and sodium hydrogen carbonate) and is suitable for cell encapsulation allowing obtaining 3D culture systems. Physico-chemical analyses showed that the hydrogel formulations jellify at physiological conditions (37 °C, pH 7.4), are stable in vitro up to three weeks, have high swelling ratios and mechanical stiffness suitable for cellular encapsulation. Moreover, preliminary biological tests underlined the pronounced biocompatibility of the system. Therefore, these chitosan-based hydrogels are proposed as valid biomaterials for cell encapsulation.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This Ch/β-GP hydrogel (2-10% w/v) was sol at 4 C and formed gel at 37 C. The biocompatibility of the hydrogel with MTT assay showed that the hydrogel, in agreement with previous studies, had no cytotoxic effects on 4 T1 cells. 32,33 The gelation time is another important item in the applications of the chitosan-based hydrogels. Various factors are involved in this process, including the percentage of chitosan and other materials used in the hydrogels.…”

Section: Discussionmentioning

confidence: 99%

A three dimensional in vivo model of breast cancer using a thermosensitive chitosan‐based hydrogel and 4 T1 cell line in Balb/c

Rezakhani

Alizadeh

2020

J Biomedical Materials Res

View full text Add to dashboard Cite

The two‐dimensional (2D) models of breast cancer still exhibit a limited success. Whereas, three‐dimensional (3D) models provide more similar conditions to the tumor for growth of cancer cells. In this regard, a 3D in vivo model of breast cancer using 4 T1 cells and chitosan‐based thermosensitive hydrogel were designed. Chitosan/β‐glycerol phosphate hydrogel (Ch/β‐GP) was prepared with a final ratio of 2% and 10%. The hydrogel properties were examined by Fourier transformed infrared spectroscopy, MTT assay, pH, scanning electron microscopy, and biodegradability assay. 3D model of breast cancer was induced by injection of 1 × 106 4 T1 cells in 100 μl hydrogel and 2D model by injection of 1 × 106 4 T1 cells in 100 μl phosphate‐buffered saline (PBS) subcutaneously. After 3 weeks, induced tumors were evaluated by size and weight determination, ultrasound, hematoxylin‐ and eosin and Masson's trichrome staining and evaluating of cancer stem cells with CD44 and CD24 markers. The results showed that hydrogel with physiological pH had no cytotoxicity. In 3D model, tumor size and weight increased significantly (p ≤ .001) in comparison with 2D model. Histological and ultrasound analysis showed that 3D tumor model was more similar to breast cancer. Expression of CD44 and CD24 markers in the 3D model was more than 2D model (p ≤ .001). This 3D in vivo model of breast cancer mimicked native tumor and showed malignant tissue properties. Therefore, the use of such models can be effective in various cancer studies, especially in the field of cancer stem cells.

show abstract

“…β-Glycerophosphate (GP) is a protein phosphatase inhibitor that acts as a phosphate group donor in matrix mineralization research and promotes bone matrix mineralization [ 91 ]. The addition of β-GP to CS aims to reduce the polarity of the chitosan chain and increase hydrophobic interactions, which leads to the production of thermosensitive hydrogels [ 92 ]. The sol-gel transition occurred at 37 ℃.…”

Section: Hydrogelsmentioning

confidence: 99%

Polymeric Carriers for Delivery Systems in the Treatment of Chronic Periodontal Disease

et al. 2020

View full text Add to dashboard Cite

Periodontitis (PD) is a chronic inflammatory disease of periodontal tissues caused by pathogenic microorganisms and characterized by disruption of the tooth-supporting structures. Conventional drug administration pathways in periodontal disease treatment have many drawbacks such as poor biodistribution, low selectivity of the therapeutic effect, burst release of the drug, and damage to healthy cells. To overcome this limitation, controlled drug delivery systems have been developed as a potential method to address oral infectious disease ailments. The use of drug delivery devices proves to be an excellent auxiliary method in improving the quality and effectiveness in periodontitis treatment, which includes inaccessible periodontal pockets. This review explores the current state of knowledge regarding the applications of various polymer-based delivery systems such as hydrogels, liposomes, micro-, and nanoparticles in the treatment of chronic periodontal disease. Furthermore, to present a more comprehensive understanding of the difficulties concerning the treatment of PD, a brief description of the mechanism and development of the disease is outlined.

show abstract

An injectable chitosan/dextran/β -glycerophosphate hydrogel as cell delivery carrier for therapy of myocardial infarction

Cited by 87 publications

References 52 publications

Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation

Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation

A three dimensional in vivo model of breast cancer using a thermosensitive chitosan‐based hydrogel and 4 T1 cell line in Balb/c

Polymeric Carriers for Delivery Systems in the Treatment of Chronic Periodontal Disease

Contact Info

Product

Resources

About