An in vitro study of the protective effect of caffeic acid on human erythrocytes

Colina, José R.; Suwalsky, Mario; Manrique-Moreno, Marcela; Petit, Karla; Aguilar, Luís Fuentes; Jemioła-Rzemińska, Małgorzata; Strzałka, Kazimierz

doi:10.1016/j.abb.2018.12.006

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…Both compounds were not toxic to human erythrocytes at all concentrations tested. These findings agree with earlier studies that indicate the absence of toxicity of these compounds in RBC and demonstrate protective roles of phenolic compounds in damaged cells [88][89][90]. Based on these promising data, we interrogated EA and CAPE using two different in vivo invertebrate models: the wax moth larva G. mellonella and the nematode C. elegans.…”

Section: Discussionsupporting

confidence: 89%

Antifungal Activity of the Phenolic Compounds Ellagic Acid (EA) and Caffeic Acid Phenethyl Ester (CAPE) against Drug-Resistant Candida auris

Rossatto

Tharmalingam

Escobar

et al. 2021

JoF

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Candida auris is an emerging healthcare-associated fungal pathogen that has become a serious global health threat. Current treatment options are limited due to drug resistance. New therapeutic strategies are required to target this organism and its pathogenicity. Plant polyphenols are structurally diverse compounds that present a vast range of biological properties. In the present study, plant-derived molecules ellagic acid (EA) and caffeic acid phenethyl ester (CAPE) were investigated for their antifungal and antivirulence activities against Candida auris. We also tested against C. albicans. The minimum inhibitory concentration (MIC) for EA ranged from 0.125 to 0.25 µg/mL and for CAPE ranged from 1 to 64 µg/mL against drug-resistant C. auris strains. Killing kinetics determined that after 4 h treatment with CAPE, there was a complete reduction of viable C. auris cells compared to fluconazole. Both compounds might act by modifying the fungal cell wall. CAPE significantly reduced the biomass and the metabolic activity of C. auris biofilm and impaired C. auris adhesion to cultured human epithelial cells. Furthermore, both compounds prolonged the survival rate of Galleria mellonella infected by C. auris (p = 0.0088 for EA at 32 mg/kg and p = 0.0028 for CAPE at 4 mg/kg). In addition, EA at 4 μg/mL prolonged the survival of C. albicans-infected Caenorhabditis elegans (p < 0.0001). CAPE was not able to prolong the survival of C. albicans-infected C. elegans. These findings highlight the antifungal and antivirulence effects of EA and CAPE against C. auris, and warrant further investigation as novel antifungal agents against drug-resistant infections.

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Section: Discussionsupporting

confidence: 89%

Antifungal Activity of the Phenolic Compounds Ellagic Acid (EA) and Caffeic Acid Phenethyl Ester (CAPE) against Drug-Resistant Candida auris

Rossatto

Tharmalingam

Escobar

et al. 2021

JoF

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“…Thymol [425] Natural polymers Lignin [426][427][428], Polyphenols [429], Cellulose [430,431], Polysialic acid [432] Gabaergic ketones Carvone, Menthone, Pulegone, Dihydrocarvone, Thujone [433] Taste and aroma Menthol [342,434], Terpenoids [435], Coumarin [132,284,436,437], Limonene [132], 4-ethylphenol (wine/beer aroma) [406], Tannins (wine) [438], Catechin [282] Caffeine and its derivatives Caffeine [132,439], Rosmarinic acid [440][441][442], Caffeic acid [441,443], Chlorogenic acid [441], Paraxanthine [132], Caffeic acid derivatives [444] Pigments Violacein [445,446], Marennine [447] Bile salts [280,423,448] Steroids Betulin [449], Saponins [450,451], Glycyrrhizic acid (saponin) [252,452,453], Withaferin-A and Withanone [454] Lipids N-arachidonylglycine and oleoyl-L-carnitine [137]...…”

Section: Antioxidantsmentioning

confidence: 99%

Mechanistic Understanding from Molecular Dynamics in Pharmaceutical Research 2: Lipid Membrane in Drug Design

2021

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We review the use of molecular dynamics (MD) simulation as a drug design tool in the context of the role that the lipid membrane can play in drug action, i.e., the interaction between candidate drug molecules and lipid membranes. In the standard “lock and key” paradigm, only the interaction between the drug and a specific active site of a specific protein is considered; the environment in which the drug acts is, from a biophysical perspective, far more complex than this. The possible mechanisms though which a drug can be designed to tinker with physiological processes are significantly broader than merely fitting to a single active site of a single protein. In this paper, we focus on the role of the lipid membrane, arguably the most important element outside the proteins themselves, as a case study. We discuss work that has been carried out, using MD simulation, concerning the transfection of drugs through membranes that act as biological barriers in the path of the drugs, the behavior of drug molecules within membranes, how their collective behavior can affect the structure and properties of the membrane and, finally, the role lipid membranes, to which the vast majority of drug target proteins are associated, can play in mediating the interaction between drug and target protein. This review paper is the second in a two-part series covering MD simulation as a tool in pharmaceutical research; both are designed as pedagogical review papers aimed at both pharmaceutical scientists interested in exploring how the tool of MD simulation can be applied to their research and computational scientists interested in exploring the possibility of a pharmaceutical context for their research.

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“…Chlorogenic acid inhibits the induction of oxidative damage in rat pheochromocytoma cells ( Yao, et al. , 2019 ), while caffeic acid protects human erythrocyte membranes from oxidizing species ( Colina et al. , 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Caffeic acid and chlorogenic acid cytotoxicity, genotoxicity and impact on global DNA methylation in human leukemic cell lines

et al. 2020

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Dietary phenolic compounds such as caffeic and chlorogenic acid exert an antiproliferative effect and modulate the gene-specific DNA methylation status in human breast tumor cells, but it remains unclear whether they interfere with global DNA methylation in human leukemia cells. We examined whether caffeic and chlorogenic acid (1-250 mM) exert antitumor action in human promyelocytic leukemia cells (HL-60) and human acute T-cell leukemia cells (Jurkat). Caffeic and chlorogenic acid did not reduce cell viability in the two cell lines, as assessed using the neutral red uptake and MTT assays. These phenolic acids (1-100 mM) neither induced DNA damage (comet assay) nor increased the micronuclei frequency (micronucleus assay) in HL-60 and Jurkat cells, indicating that they were not genotoxic or mutagenic. Analysis of global DNA methylation levels using a 5-mC DNA ELISA kit revealed that chlorogenic acid at a non-cytotoxic concentration (100 mM) induced global DNA hypomethylation in Jurkat cells, but not in HL-60 cells, suggesting that it exerts a cell-specific effect. Caffeic acid did not change global DNA methylation. As other phenolic compounds, chlorogenic acid probably modulates DNA methylation by targeting DNA methyltransferases. The hypomethylating action of chlorogenic acid can be beneficial against hematological malignances whose pathogenic processes involve impairment of DNA methylation.

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An in vitro study of the protective effect of caffeic acid on human erythrocytes

Cited by 16 publications

References 39 publications

Antifungal Activity of the Phenolic Compounds Ellagic Acid (EA) and Caffeic Acid Phenethyl Ester (CAPE) against Drug-Resistant Candida auris

Antifungal Activity of the Phenolic Compounds Ellagic Acid (EA) and Caffeic Acid Phenethyl Ester (CAPE) against Drug-Resistant Candida auris

Mechanistic Understanding from Molecular Dynamics in Pharmaceutical Research 2: Lipid Membrane in Drug Design

Caffeic acid and chlorogenic acid cytotoxicity, genotoxicity and impact on global DNA methylation in human leukemic cell lines

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