An In Vitro Human Lens Capsular Bag Model Adopting a Graded Culture Regime to Assess Putative Impact of IOLs on PCO Formation

Eldred, Julie A.; Jiao, Zheng; Chen, Sulin; Wormstone, I. Michael

doi:10.1167/iovs.18-25930

Cited by 28 publications

(41 citation statements)

References 19 publications

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“…In addition, cells are less likely to populate the IOL surface of the Vivinex IOL than the AcrySof IOL, which further reduces light scatter following surgery. 36 Two other disadvantages of hydrophobic acrylic IOLs are more frequent dysphotopsias related to a low anterior curvature and a high refractive index and the development of glistenings: microvacuoles within the IOL material may occur when the IOL is in an aqueous environment and water fills microscopic openings within the material. Typical for acrylic IOLs, glistenings appear as white sparkling areas all over the IOL optic, which may impair the optical quality.…”

Section: Three Years Postoperatively the Vivinex Xy1 Iolsmentioning

confidence: 99%

Posterior Capsule Opacification With Two Hydrophobic Acrylic Intraocular Lenses: 3-Year Results of a Randomized Trial

Leydolt

Schartmüller

Röggla

et al. 2020

American Journal of Ophthalmology

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To compare the incidence and intensity of posterior capsule opacification (PCO) and neodymiumyttrium-aluminum-garnet (Nd:YAG) capsulotomy rates between 2 similar open-loop single-piece hydrophobic acrylic intraocular lenses (IOLs) that differ in the proprietary material characteristics and design features, over a period of 3 years.DESIGN: Randomized, prospective, patient-and examiner-masked clinical trial with intraindividual comparison.

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Section: Three Years Postoperatively the Vivinex Xy1 Iolsmentioning

confidence: 99%

Posterior Capsule Opacification With Two Hydrophobic Acrylic Intraocular Lenses: 3-Year Results of a Randomized Trial

Leydolt

Schartmüller

Röggla

et al. 2020

American Journal of Ophthalmology

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“…37 In addition, a graded culture regime for the in vitro human lens capsular bag model that better reflects the clinical events following cataract surgery should be established in future studies. 38 Due to the in vitro character of this study, further studies have to determine whether the short and early release of gefitinib by the IOL is sufficient to decelerate PCO formation longer-term.…”

Section: Discussionmentioning

confidence: 99%

Development of a drug-eluting intraocular lens to deliver epidermal growth factor receptor inhibitor gefitinib for posterior capsule opacification prophylaxis

Kassumeh

Kueres

Hillenmayer

et al. 2019

European Journal of Ophthalmology

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Purpose: Different molecular targets, such as the epidermal growth factor receptor, have been identified for the prophylaxis of posterior capsule opacification. This led to the proposal of several drugs, yet drug delivery into the capsular bag remains challenging. The intraocular lens as a drug delivery device would provide a convenient method to allow drug release in the location needed. This is to evaluate the effect of a drug-eluting intraocular lens using an epidermal growth factor receptor inhibitor. Methods: Hydrophobic and hydrophilic intraocular lenses were coated with gefitinib using the dip coating technique. The cellular response on the modified intraocular lenses was tested in a human lens epithelial cell line (FHL-124) in an anterior segment model. Furthermore, modified intraocular lenses were implanted into human capsular bags ex vivo. Drug release was determined as well as the biocompatibility on human corneal endothelial cells. Unmodified intraocular lenses served as controls. In addition, immunofluorescence staining with fibronectin as a marker for fibrotic response was conducted. Results: Both coated hydrophilic and hydrophobic intraocular lenses could attenuate the cell growth of FHL-124 cells in the human capsular bag in comparison to the unmodified controls. Furthermore, gefitinib-soaked intraocular lenses showed a constant drug release over the first 10 days. No reduction in cell viability of corneal endothelial cells occurred. A decrease in fibronectin expression under gefitinib treatment could be observed. Conclusion: In vitro epidermal growth factor receptor seems to be a valuable target for the prevention of posterior capsule opacification. The gefitinib-eluting intraocular lens in this study could inhibit cell growth in non-toxic concentrations.

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“…With the addition of SIS3, the staining of E-cadherin did not alter in comparison to the untreated control explants and only a partial reduction in β-catenin nuclear translocation was seen. Ex vivo models have also been used to improve the reproduction of the clinical environment of PCO as shown by Eldred [123] who used a graded culture media in a human capsular bag model to assess the impact of IOL design on its propensity to initiate PCO development. Mock surgeries were completed on donor human eye tissue after which the eyes were implanted with either Hoya Vivinex or Alcon Acrysof lenses.…”

Section: Ex Vivo Modelsmentioning

confidence: 99%

Factors Affecting Posterior Capsule Opacification in the Development of Intraocular Lens Materials

Cooksley

Lacey²,

Dymond

et al. 2021

Pharmaceutics

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Posterior capsule opacification (PCO) is the most common complication arising from the corrective surgery used to treat cataract patients. PCO arises when lens epithelial cells (LEC) residing in the capsular bag post-surgery undergo hyper-proliferation and transdifferentiation into myofibroblasts, migrating from the posterior capsule over the visual axis of the newly implanted intraocular lens (IOL). The developmental pathways underlying PCO are yet to be fully understood and the current literature is contradictory regarding the impact of the recognised risk factors of PCO. The aim of this review is firstly to collate the known biochemical pathways that lead to PCO development, providing an up-to-date chronological overview from surgery to established PCO formation. Secondly, the risk factors of PCO are evaluated, focussing on the impact of IOLs’ properties. Finally, the latest experimental model designs used in PCO research are discussed to demonstrate the ongoing development of clinical PCO models, the efficacy of newly developed IOL technology, and potential therapeutic interventions. This review will contribute to current PCO literature by presenting an updated overview of the known developmental pathways of PCO, an evaluation of the impact of the risk factors underlying its development, and the latest experimental models used to investigate PCO. Furthermore, the review should provide developmental routes for research into the investigation of potential therapeutic interventions and improvements in IOL design in the aid of preventing PCO for new and existing patients.

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An In Vitro Human Lens Capsular Bag Model Adopting a Graded Culture Regime to Assess Putative Impact of IOLs on PCO Formation

Abstract: Wormstone IM. An in vitro human lens capsular bag model adopting a graded culture regime to assess putative impact of IOLs on PCO formation.

Cited by 28 publications

References 19 publications

Posterior Capsule Opacification With Two Hydrophobic Acrylic Intraocular Lenses: 3-Year Results of a Randomized Trial

Posterior Capsule Opacification With Two Hydrophobic Acrylic Intraocular Lenses: 3-Year Results of a Randomized Trial

Development of a drug-eluting intraocular lens to deliver epidermal growth factor receptor inhibitor gefitinib for posterior capsule opacification prophylaxis

Factors Affecting Posterior Capsule Opacification in the Development of Intraocular Lens Materials

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