An Improved Method for the Fluorochrome Staining of Mycobacteria in Tissues and Smears

Re, Mansfield

doi:10.1093/ajcp/53.3.394

Cited by 19 publications

(10 citation statements)

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“…4 Auramine Phenol Fluorescent Method Mansfield (1970) compared 14 different fluorescent methods for the detection of tubercle bacilli in histological sections and found the auramine phenol stain to be the most satisfactory.…”

Section: Resultsmentioning

confidence: 99%

“…As in all fluorescent methods, artefacts present a constant problem, but as Mansfield (1970) points out, fluorescent staining techniques have been improved consiclorably in recent years, with the virtual elimination of tissue fluorescence, reducing the problem of artefacts to a minimum. In the present study, bacterial morphology showed up clearly and only morphologically definite bacilli were counted as positive.…”

Section: Discussionmentioning

confidence: 99%

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A comparison of methods for staining tubercle bacilli in histological sections

Greenwood

Fox

1973

J Clin Pathol

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SYNOPSIS The Ziehl-Neelsen stain is compared with three alternative methods of staining tubercle bacilli in paraffin sections: Fite's method (1938); a modification by Armstrong and Price (1947) of Fite's method; and a fluorescent method using the auramine-phenol stain.The fluorescent method was found to be the most effective of the four methods and its use is recommended as a routine tool in diagnostic histopathology.The Ziehl-Neelsen stain is the most commonly used technique in Britain for the demonstration of tubercle bacilli in histological sections. In our experience, however, this stain yields a disquietingly low incidence of positive results in sections from lesions which are histologically typical of tuberculosis. In such cases, a diagnosis of tuberculosis is often made in spite of the failure to demonstrate the organism, although a slight doubt as to the true nature of the lesion may remain in the pathologist's mind.Any technique which produces better results than those obtained with the Ziehl-Neelsen stain would therefore be welcome to histologists. This investigation involves a comparison of the Ziehl-Neelsen stain with two alternative fuchsin stains and with a fluorescent technique in an attempt to determine which is of the most value in the routine examination of histological sections. MaterialSeventy cases which had been diagnosed as 'tuberculosis' were selected at random from the files of the Department of Pathology, University of Manchester. Sections from all these cases showed epithelioid cell granulomata and areas of caseation. The tissues were from a variety of anatomical sites including lymph nodes (21), kidney (9), gastrointestinal tract (6), epididymus (5), liver (4), omentum (4), synovium (3), brain (1), vagina (1), heart (1), spleen (1), and skin (1).Sections from each case were cut and stained by the following methods:

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A comparison of methods for staining tubercle bacilli in histological sections

Greenwood

Fox

1973

J Clin Pathol

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“…The Ziehl-Neelsen stain requires heat; the Kinyoun stain, in which the concentrations of carbol fuchsin and phenol are increased, does not. The third method uses the fluorochrome dye auramine O with or without rhodamine (12,116,177). Smears stained with carbol fuchsin are examined by light microscopy under oil immersion, whereas those stained with auramine O are examined by fluorescence microscopy at lower magnifications (ϫ150 and ϫ450).…”

Section: Stains For Detection Of Mycobacteriamentioning

confidence: 99%

Detection of infection or infectious agents by use of cytologic and histologic stains

1996

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A wide variety of stains are useful for detection of different organisms or, for viruses, the cytopathologic changes they induce, in smears prepared directly from clinical specimens and in tissue sections. Other types of stains, such as hematoxylin and eosin, are used routinely to stain tissue sections and are most valuable for assessing the immunologic response of the host to the invading pathogen. In many cases, the pattern of inflammation provides important clues to diagnosis and helps to guide the selection of additional "special" stains used predominantly for diagnosis of infectious diseases. A stain may be nonspecific, allowing detection of a spectrum of organisms, as do the Papanicolaou stain and silver impregnation methods, or detection of only a limited group of organisms, as do the different acid-fast techniques. Some nonspecific stains, such as the Gram stain, are differential and provide valuable preliminary information concerning identification. Immunohistochemical stains, on the other hand, are specific for a particular organism, although in some cases cross-reactions with other organisms occur. Despite the wealth of information that can be gleaned from a stained smear or section of tissue, however, the specific etiology of an infection often cannot be determined on the basis of only the morphology of the organisms seen; culture data are essential and must be considered in the final diagnosis.

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“…The tissues were fixed in buffered formaldehyde solution, sections were cut at 6 pm, stained with haematoxylin and eosin, followed by the Fite-Feraco modification of Ziehl-Neelsen. Selected tissues from some animals, apparently negative for bacilli following Ziehl-Neelsen staining, were stained with auromine phenol (Mansfield, 1970), and scanned under blue light, using BG12 and BG38 filters to give a mean excitation of 460 nm.…”

Section: Histopathologymentioning

confidence: 99%

The testis in mice infected with Mycobacterium leprae

Rees

McDougall

Weddell

1975

The Journal of Pathology

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Following inoculation either locally or intravenously with Mycobact. leprae of human origin, the histopathology and bacteriology of the testis in experimental mice is described. Normal mice, and mice rendered immunologically deficient by thymectomy and whole-body irradiation, were studied. Attention is drawn to a heavy bacillation of the testis in mice from both groups. Bacilli were found in and beneath the tunica albuginea, but mainly in interstitial cells and in macrophages surrounding the tubules. The percentage of solidly staining bacilli was high, and globi were frequent. The study showed that the testis in mice is particularly favourable for the lodgement and multiplication of Mycobact. laprae following either local or intravenous inoculation. The significance of this in relation to the metabolism of the leprosy bacillus and to the frequent occurrence of testicular damage in the lepromatous male patient is discussed. This work was supported by grants to A. G. M. Weddell and A. C. McDougall from the Medical Research Council and the British Leprosy Relief Association (LEPRA).

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An Improved Method for the Fluorochrome Staining of Mycobacteria in Tissues and Smears

Cited by 19 publications

References 0 publications

A comparison of methods for staining tubercle bacilli in histological sections

A comparison of methods for staining tubercle bacilli in histological sections

Detection of infection or infectious agents by use of cytologic and histologic stains

The testis in mice infected with Mycobacterium leprae

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