A comparison of methods for staining tubercle bacilli in histological sections

Greenwood, N.M.; Fox, And H.

doi:10.1136/jcp.26.4.253

Cited by 15 publications

(6 citation statements)

References 5 publications

(4 reference statements)

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“…Staining by AP method exhibited a high sensitivity compared to that of ZN corroborating with the earlier findings when the AP stain was applied to liquefied, concentrated samples (Murray et al 2003), CPC treated samples (RNTCP 1998) and histological sections (Greenwood and Fox 1973).…”

Section: Discussionsupporting

confidence: 80%

An alternative sputum processing method using chitin for the isolation of Mycobacterium tuberculosis

Balaji

Dusthackeer

Rehman

et al. 2009

World J Microbiol Biotechnol

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An alternative bio-friendly sputum processing method is the need of the hour to augment the rate of detection of TB cases and to improve the sensitivity of rapid growth based diagnostic methods. Chitin, mucolytic in nature and present ubiquitously in animal kingdom, was found to have decontaminating activity when used for processing sputum specimens. The aim of the present study is to develop an alternative bio friendly sputum processing method using chitin. Smear microscopy was done on direct sputum samples and on the deposits obtained after processing with modified Petroff's method as well as Chitin method. Two direct smears were made from each of the sputum samples and stained by Ziehl Neelsen and Auramine phenol (AP) method. The samples were divided in to two aliquots and processed by chitin and modified Petroff's method. Smears were made from each of the deposits and stained by both methods. The deposits were inoculated on to two Lowenstein Jensen slopes. AP method showed a sensitivity of 95% in direct smear. Samples processed by chitin and the deposit smears stained by AP method showed a sensitivity of 80% and a specificity of 89% compared to that of modified Petroff's method. The sensitivity of chitin culture is 87% and the specificity is 85%. Chitin-H 2 So 4 solution took less time compared to 4% NaOH to homogenize the mucopurulent sputum specimens. Chitin-H 2 So 4 can be used as an alternative method of sputum processing for the detection of M. tuberculosis.

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Section: Discussionsupporting

confidence: 80%

An alternative sputum processing method using chitin for the isolation of Mycobacterium tuberculosis

Balaji

Dusthackeer

Rehman

et al. 2009

World J Microbiol Biotechnol

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“…Histopathologically, unspecific rather than pathognomonic signs, such as acute inflammation, microabscesses, granulomas (with or without caseation), and/or reactive vasculopathy, are seen using routine staining, such as haematoxylin and eosin (1-3, 5, 6). Acid-fast staining with, for example, Ziehl-Neelsen, will be needed to identify bacilli (33,34). Fites' acid-fast stain, as performed at our centre, proved to be an effective method, which uses lower alcohol concentrations and a milder destaining acid compared with classical acid-fast bacilli staining (33,34).…”

Section: Discussionmentioning

confidence: 94%

“…Acid-fast staining with, for example, Ziehl-Neelsen, will be needed to identify bacilli (33,34). Fites' acid-fast stain, as performed at our centre, proved to be an effective method, which uses lower alcohol concentrations and a milder destaining acid compared with classical acid-fast bacilli staining (33,34). In addition, appropriate bacterial culture determines the correct mycobacteria species (1-3, 5, 6).…”

Section: Discussionmentioning

confidence: 99%

Skin and Soft Tissue Infections Caused by Mycobacterium chelonae: More Common Than Expected?

Uslu¹,

Böhm²,

Heppt³

et al. 2019

Acta Derm Venerol

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Patients can present with infections of the skin and soft tissues due to the pathogen Mycobacterium chelonae. Clinical presentation of this relatively rare infectious disease may differ from individual to individual. Despite an increasing incidence, affected patients are often misdiagnosed. Thus, in addition to careful history-taking, tissue biopsies for mycobacterial culture and histopathological examination are needed to reach a correct diagnosis. Once diagnosed, culture-directed antibiotic therapy is required. We report here 5 patients with cutaneous and/or soft-tissue infections due to M. chelonae, who were diagnosed and treated appropriately at our centre. Mycobacterium chelonae is a rapidly growing nontuber culous mycobacterium, which causes infections of the human skin and soft tissue. Despite an increasing incidence of such infections, patients are often misdiagnosed. We report here 5 patients with cutaneous and/ or soft tissue infection due to M. chelonae who were diagnosed and treated at our centre. Two of the 5 patients were on immunosuppressive treatment. While clinical presentations differed in each patient, all had a long history of skin lesions. In addition to careful history-taking, tissue biopsies were obtained for mycobacterial culture and histopathological examination. Culturedirected antibiotic therapy was initiated, which resulted in a slow, but continuous, healing of the lesions. In summary, M. chelonae infections are still relatively rare, but should be considered in both immunocompromised and immunocompetent patients with prolonged skin lesions resistant to standard antibiotic treatment. For diagnosis, tissue analysis for mycobacterial culture and histopathological examination, and once diagnosed, adequate antibiotic treatment, is needed.

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“…Fine-needle aspiration from lymph nodes was performed by local clinicians or pathologists and cell smears for cytology were directly prepared and fixed after sample collection. If microscopy for AFB was requested by the pathologist, the Ziehl-Neelsen (HUH, SUH), Auramine (OUS) or Fite Faraco [36] (OUS) method was used. Additionally, a previously developed in-house nested-PCR (n-PCR) for detection of the MTBC-specific IS6110 sequence in DNA extracted from archived material [37] was also performed on the samples as part of routine diagnostics at HUH only, if requested by the pathologist.…”

Section: Sample Processing and Routine Tb Diagnostic Proceduresmentioning

confidence: 99%

Diagnosis of extrapulmonary tuberculosis using the MPT64 antigen detection test in a high-income low tuberculosis prevalence setting

et al. 2020

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Background: Extrapulmonary tuberculosis (EPTB) poses diagnostic challenges due to the paucibacillary nature of the disease. The immunochemistry-based MPT64 antigen detection test (MPT64 test) has shown promising results for diagnosing EPTB in previous studies performed in low-resource settings, with higher sensitivity than microscopy and culture. The aim of this study was to investigate the performance of the MPT64 test in a routine clinical setting in a high-income low TB prevalence country. Methods: Extrapulmonary samples sent for TB diagnostics to microbiology and pathology laboratories at three regional tertiary care hospitals in Norway in a one-year period were included and subjected to the MPT64 test in parallel to the routine TB diagnostic tests. Results: Samples from 288 patients were included and categorised as confirmed TB cases (n = 26), clinically diagnosed TB cases (n = 5), non-TB cases (n = 243) and uncategorised (n = 14), using a composite reference standard (CRS). In formalin-fixed biopsies, the sensitivity (95% CI) of the MPT64 test, microscopy, PCR-based tests pooled, and culture was 37% (16-62), 20% (4-48), 37% (16-62) and 50% (23-77), respectively, against the CRS. The MPT64 test showed a good positive predictive value (88%) and an excellent specificity (99, 95% CI 92-100) in formalin-fixed biopsies. In fine-needle aspirates, pus and fluid samples, the test performance was lower. Conclusions: The MPT64 test was implementable in pathology laboratories as part of routine diagnostics, and although the sensitivity of the MPT64 test was not better than culture in this setting, the test supplements other rapid diagnostic methods, including microscopy and PCR-based tests, and can contribute to strengthen the diagnosis of EPTB in formalin-fixed biopsies in the absence of culture confirmation.

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A comparison of methods for staining tubercle bacilli in histological sections

Cited by 15 publications

References 5 publications

An alternative sputum processing method using chitin for the isolation of Mycobacterium tuberculosis

An alternative sputum processing method using chitin for the isolation of Mycobacterium tuberculosis

Skin and Soft Tissue Infections Caused by Mycobacterium chelonae: More Common Than Expected?

Diagnosis of extrapulmonary tuberculosis using the MPT64 antigen detection test in a high-income low tuberculosis prevalence setting

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