An improved method for enzymic determination of glucose in the presence of maltose

Lloyd, John; Whelan, W. J.

doi:10.1016/0003-2697(69)90143-2

Cited by 526 publications

(146 citation statements)

References 4 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…The concentrations of dextran and maltose solutions were determined by acid hydrolysis [9] followed by estimation of glucose released with glucose oxidase [10] Dextransucrase suspension (1.0 unit) was incubated at 25 ° in digests (2 ml) containing 14C-sucrose (40 mg, 4.2 X l0 s cpm) and sodium acetate (0.06 M) buffer, pH 5.0. Samples (0.2 ml) were removed at intervals and made to 1.0 ml with water.…”

Section: Methodsmentioning

confidence: 99%

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

Smith

1970

FEBS Letters

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

Smith

1970

FEBS Letters

View full text Add to dashboard Cite

“…Concentrations of glucose and sucrose in the IWF were also measured before and after invertase hydrolysis by the glucose oxidase (GOD) assay using the method of Lloyd & Whelan (1969).…”

Section: Sugar Analysis Of the Iwfmentioning

confidence: 99%

Effect of powdery mildew infection on concentrations of apoplastic sugars in pea leaves

Aked

Hall

1993

New Phytologist

View full text Add to dashboard Cite

SUMMARYIntercellular washing fluid (IWF) was extracted by centrifugation from healthy and mildew-infected pea leaves. The level of cytoplasmic contamination of the IWF was estimated to be 0-3 "o for healthy leaves and 0-7 % in mildew-infected leaves as assessed by the presence of malate dehydrogenase activity. The IWF was analyzed for sugars using gas liquid chromatography and the glucose oxidase assay. Changes in the concentrations of glucose, fructose and sucrose showed different trends over an infection time course. Glucose and sucrose concentrations fluctuated over the 7 d period following infection but almost always showed higher levels in infected tissues than in the controls. Fructose concentrations were low and showed a greater increase over 7 d in infected compared to healthy leaves. The pattern of efflux of sucrose from pre-loaded healthy and mildew-infected pea leaf discs suggests that the plasma membranes of cells from infected pea leaves might be more leaky than those of healthy pea leaves. The implications of apoplastic sugar levels for nutritional aspects of the plant-pathogen interaction are discussed.

show abstract

“…A brief review of the development of the glucose oxidase method and the results of a collaborative trial on the procedure have been given by Brady and Zagorski.18 The activity of con taminating enzymes, chiefly maltase, can be overcome by performing the reaction in a Tris-phosphatc-glycerol buffer, as described by Lloyd and Whelan. 88 The present authors consider this to be a very precise and convenient method for the estimation of glucose in beers, worts and hydrolysates.…”

Section: Enzymic Proceduresmentioning

confidence: 88%

Measurement of Carbohydrates in Wort and Beer-a Review

Buckee¹,

Hargitt²

1978

Journal of the Institute of Brewing

View full text Add to dashboard Cite

Methods are reviewed for measuring total wort and beer carbohydrate and carbohydrate frac tions, such as dextrins, oligosaccharides, fermentable sugars, jS-glucans, total fructose and fructosans, pentose and pentosans. The methods are conveniently classified under the following headings: reductometry, colorimetry, enzymic procedures, automated analyses, paper and column chromatography, thin layer chromatography, gas liquid chromatography and high performance liquid chromatography. Techniques involving chromatography are particularly useful for separating and estimating individual sugars.

show abstract

An improved method for enzymic determination of glucose in the presence of maltose

Cited by 526 publications

References 4 publications

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

Effect of powdery mildew infection on concentrations of apoplastic sugars in pea leaves

Measurement of Carbohydrates in Wort and Beer-a Review

Contact Info

Product

Resources

About