Biosynthetic relation between the soluble and insoluble dextrans produced by <i>Leuconostoc mesenteroides</i> NRRL B‐1299

Smith, Eric E.

doi:10.1016/0014-5793(70)80588-9

Cited by 36 publications

(15 citation statements)

References 14 publications

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“…Higher levels (68.46%) of soluble dextransucrase activity detected in the culture supernatant are in agreement with the extracellular localization of most dextransucrases produced by lactic-acid bacteria belonging to the genus Leuconostoc [24,37]. In L. mesenteroides NRRL B-1299 (ATCC 11449) an insoluble form of dextransucrase is responsible for the 60-95% of the whole glucosyltransferase (dextransucrase) activity produced [18,33,39]. The L. citreum NRRL B-1355 strain produces an alternansucrase (EC 2.4.140), that is predominantly cell-associated and it represents less than 10% of the whole glucosyltransferase activity in the supernatant fraction of active young cultures [45].…”

Section: Resultssupporting

confidence: 57%

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

Vidal¹,

Martínez²,

Moulis³

et al. 2011

J Ind Microbiol Biotechnol

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The industrial Leuconostoc strain B/110-1-2 producing dextran and dextran derivatives was taxonomically identified by 16S rRNA as L. citreum. Its dextransucrase enzymes were characterized according to their cellular location and reaction specificity. In the presence of sucrose, the strain B/110-1-2 produced two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46%) at 160-180 kDa. Two open reading frames (ORF) coding for L. citreum strain B/110-1-2 dextransucrases were identified. One of them shared a 52% identity with the alternansucrase ASR of L. citreum NRRL B-1355 and with a putative annotated alternansucrase sequence found in the genome of L. citreum KM20. The structural analysis (HPAEC-PAD, HPSEC, and (13)C-NMR) of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases suggest this novel glucansucrase has specificity similar to a dextransucrase but not to an alternansucrase, producing a soluble linear dextran with glucose molecules linked mainly in α-1,6 and α-1,3 with α-1,4 branches. These results enhance the understanding of this industrially significant strain and will aid in distinguishing between physiologically similar Leuconostoc spp. strains.

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Section: Resultssupporting

confidence: 57%

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

Vidal¹,

Martínez²,

Moulis³

et al. 2011

J Ind Microbiol Biotechnol

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“…Cette couche superficielle des cellules croissant en présence de saccharose, représente un complexe dextrane fixé à la cellule. Les cellules ne présentant pas cette capsule produisent du dextrane relativement soluble ce qui avait été déjà noté par SMITH(1970 Ces différences morphologiques correspondent à des différences nettes dans la viscosité mesurée après incubation en bouillon saccharosé à 10 % (quelques centipoises pour les Leuconostocs de la première catégorie, viscosité supérieure à 30 centipoises pour les autres). Les souches du 2 e groupe ne représentent qu'une petite fraction des Leuconostocs producteurs de dextrane isolés des produits laitiers, de l'ordre de 2 à 3 %.…”

Section: Production De Dextranesunclassified

Les Leuconostocs. Propriétés : leur rôle en technologie laitière

Devoyod

Françoise

1988

Lait

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“…In the case of L. mesenteroides NRRL B-1299, two exocellular forms of dextransucrase are synthesized: a soluble form, which accounts for 10% of the total activity and an insoluble cell-associated form (Dols et al, 1997a;RemaudSimeon et al, 1994;Smith, 1970). Both soluble and insoluble dextransucrases can be easily entrapped in alginate beads.…”

Section: Introductionmentioning

confidence: 97%

Reactor optimization for α‐1,2 glucooligosaccharide synthesis by immobilized dextransucrase

Dols‐Lafargue

Willemot

Monsan

et al. 2001

Biotech & Bioengineering

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The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of diffusion limitation at the batch conditions used, the enzyme behavior is modified by entrapment so that the dextran yield increases and the alpha-1,2 glucooligosaccharides (GOS) are produced with a lower yield (46.6% instead of 56.7%) and have a lower mean degree of polymerization than with the free dextransucrase. When the immobilized catalyst is used in a continuous reaction, the reactor flow rate necessary to obtain high conversion of the substrates is very low, leading to external diffusion resistance. As a result, dextran synthesis is even higher than in the batch reaction, and its accumulation within the alginate beads limits the operational stability of the catalyst and decreases glucooligosaccharide yield and productivity. This effect can be limited by using reactor columns with length to diameter ratio > or =20, and by optimizing the substrate concentrations in the feed solution: the best productivity obtained was 3.74 g. U(-1). h(-1), with an alpha-1,2 GOS yield of 36%.

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Biosynthetic relation between the soluble and insoluble dextrans produced by Leuconostoc mesenteroides NRRL B‐1299

Cited by 36 publications

References 14 publications

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives

Les Leuconostocs. Propriétés : leur rôle en technologie laitière

Reactor optimization for α‐1,2 glucooligosaccharide synthesis by immobilized dextransucrase

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