An Improved Interface for Coupling Solid-Phase Microextraction (SPME) to High Performance Liquid Chromatography (HPLC) Applied to the Analysis of Explosives

Wu, Lei; Almirall, José R.; Furton, Kenneth G.

doi:10.1002/(sici)1521-4168(19990501)22:5<279::aid-jhrc279>3.0.co;2-s

Cited by 31 publications

(6 citation statements)

References 13 publications

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“…Routine sample clean-up methods prior to analysis include liquid extraction (LE) with sonication [24], centrifugation [17], accelerated solvent extraction (ASE), supercritical fluid extraction (SFE) and pressurized fluid extraction (PFE) [3,23,25]. These procedures are in general time consuming, require an expert operator and are consequently are more difficult to perform onsite [26,27].…”

Section: Introductionmentioning

confidence: 99%

Capillary-driven microfluidic paper-based analytical devices for lab on a chip screening of explosive residues in soil

Ueland

Blanes

Taudte

et al. 2016

Journal of Chromatography A

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A novel microfluidic paper-based analytical device (μPAD) was designed to filter, extract, and pre-concentrate explosives from soil for direct analysis by a lab on a chip (LOC) device. The explosives were extracted via immersion of wax-printed μPADs directly into methanol soil suspensions for 10min, whereby dissolved explosives travelled upwards into the μPAD circular sampling reservoir. A chad was punched from the sampling reservoir and inserted into a LOC well containing the separation buffer for direct analysis, avoiding any further extraction step. Eight target explosives were separated and identified by fluorescence quenching. The minimum detectable amounts for all eight explosives were between 1.4 and 5.6ng with recoveries ranging from 53-82% from the paper chad, and 12-40% from soil. This method provides a robust and simple extraction method for rapid identification of explosives in complex soil samples.

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Section: Introductionmentioning

confidence: 99%

Capillary-driven microfluidic paper-based analytical devices for lab on a chip screening of explosive residues in soil

Ueland

Blanes

Taudte

et al. 2016

Journal of Chromatography A

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“…Fiber‐based SPME was combined with HPLC to solve the problem. But a special interface must be used to fix the SPME fiber and achieve desorption and sampling with mobile phase . Except additional special interface, the analysis process is complicated and offline.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured‐silver‐coated polyetheretherketone tube for online in‐tube solid‐phase microextraction coupled with high‐performance liquid chromatography

Sun

Feng

et al. 2015

J of Separation Science

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Polyetheretherketone tube is a better substrate for in-tube solid-phase microextraction than fused-silica capillary and metal tube because of its resistance to high pressure and good flexibility. It was modified with a nanostructured silver coating, and characterized by scanning electron microscopy and energy dispersive X-ray spectroscopy. It was connected into high-performance liquid chromatography equipment to build the online analysis system by replacing the sample loop of a six-port injection valve. To get the highest extraction capacity, the preparation conditions of the coating was investigated. Important extraction conditions including length of tube, sample volume, and desorption time were optimized using eight polycyclic aromatic hydrocarbons as model analytes. The tube exhibits excellent extraction efficiency toward them, with enrichment factors from 52 to 363. The online analysis method provides good linearity (0.5-100 or 1.0-100 μg/L) and low detection limits (0.15-0.30 μg/L). It has been used to determine polycyclic aromatic hydrocarbons in water samples, with relative recoveries in the range of 92.3-120%. The tube showed highest extraction ability for polycyclic aromatic hydrocarbons, higher extraction ability for hydrophobic phthalates and anilines, and almost no extraction ability for low hydrophobic phenols, due to the possible extraction mechanism including hydrophobic and electron-rich element-metal interactions.

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“…3,4 However, only a limited number of reports on the coupling of this extraction method with LC separation systems have been published, due to the difficulties of the on-line coupling of these methods and their subsequent operation. [5][6][7][8][9][10][11] The analysis of antidepressants in human fluids is important in toxicological, forensic and pharmaceutical research. Antidepressants such as amitriptyline and imipramine are widely used in psychiatry and these compounds are metabolized in the liver mainly to nortriptyline and desipramine, respectively, these metabolites also have an antidepressant activity.…”

Section: Introductionmentioning

confidence: 99%

“…The analytes extracted are desorbed by simply passing a very small amount of an organic solvent into the capillary in a similar manner. The absence of the desorption device which is needed for conventional SPME-LC [5][6][7][8][9][10][11] and SPME-MEKC 20,21 systems makes it possible to eliminate a difficult handling stage and to reduce the volume of the organic solvent required for the desorption process. Furthermore a wide variety of polymer coatings, i.e.…”

Section: Introductionmentioning

confidence: 99%

Direct coupling of microcolumn liquid chromatography with in-tube solid-phase microextraction for the analysis of antidepressant drugs

Saito

Kawazoe

Hayashida

et al. 2000

Analyst

124

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The direct coupling of in-tube solid-phase microextraction (in-tube SPME) with microcolumn liquid chromatography (micro-LC) has been investigated for the analysis of antidepressants in human urine. The use of in-tube SPME has been clearly shown to be advantageous for the on-line coupling of the SPME method, as the sample pretreatment technique, with micro-LC as the separation technique. This is because much smaller amounts of the sample solutions, desorption solvents and the mobile phase are required compared with conventional SPME-LC systems. The parameters for preconcentration have been investigated for the extraction capillary with the newly developed 'wire-in-tube' configuration.

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An Improved Interface for Coupling Solid-Phase Microextraction (SPME) to High Performance Liquid Chromatography (HPLC) Applied to the Analysis of Explosives

Cited by 31 publications

References 13 publications

Capillary-driven microfluidic paper-based analytical devices for lab on a chip screening of explosive residues in soil

Capillary-driven microfluidic paper-based analytical devices for lab on a chip screening of explosive residues in soil

Nanostructured‐silver‐coated polyetheretherketone tube for online in‐tube solid‐phase microextraction coupled with high‐performance liquid chromatography

Direct coupling of microcolumn liquid chromatography with in-tube solid-phase microextraction for the analysis of antidepressant drugs

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