An improved HPLC method for determination of carotenoids in human serum

Rajendran, Vijayalakshmi; Pu, Yeong-Shiau; Chen, B H

doi:10.1016/j.jchromb.2005.07.004

Cited by 44 publications

(50 citation statements)

References 39 publications

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“…The calculated LOD (0.38-0.91 μg mL -1 ) and LOQ (1.15-2.77 μg mL -1 ) were similar to those of β-carotene and lycopene (LOD: 0.127-0.511 μg mL -1 and LOQ: 0.511-1.55 μg mL -1 ) reported by Rajendran et al 27 These authors also calculated the limits using parameters of the analytical curves, but another solvent system consisting of MeOH and dichloromethane was applied for carotenoid separation in HPLC. 27 On the other hand, LOD and LOQ values reported by Chisté and Mercadante 18 were lower (LOD: 0.1 μg mL -1 and LOQ: 0.5 μg mL -1 ) for a set of carotenoids (i.e., (9Z)-neoxanthin, (all-E)-violaxanthin, (all-E)-antheraxanthin, (all-E)-lutein, (all-E)-zeaxanthin, and (all-E)-β-carotene) using the same equipment and similar mobile phases and gradient elution as applied in the present work. In the latter work, authors also calculated the limits from the parameters of analytical curves; however, different concentration ranges of carotenoids were evaluated, which could have influenced the calculated limits.…”

Section: Resultssupporting

confidence: 82%

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Petry¹,

Mercadante²

2017

J. Braz. Chem. Soc.

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Carotenoid analysis in citrus is still a challenge due to their complex carotenoid profile and matrix acidity, which can promote isomerization and epoxide-furanoid rearrangements of these compounds. A new method for carotenoid extraction from freeze-dried citrus was developed. Extraction was carried out by magnetic stirring using ethyl acetate and methanol as solvents and Na 2 CO 3 to neutralize organic acids. Liquid-liquid partition was achieved by centrifugation. Carotenoid quantification was accomplished by both UV-Vis and high-performance liquid chromatography-diode array detection (HPLC-DAD). Method repeatability (2.9 to 8.5%) and recovery (82 to 88%) were similar to reported values for procedures including all the carotenoid analysis steps. In 'Valencia' orange pulp, xanthophylls accounted for 98% of the total carotenoid content and the predominant carotenoids were (9Z)-violaxanthin, (all-E)-lutein and (9Z)-antheraxanthin. This method was further applied for carotenoid analysis in freeze-dried 'Tommy Atkins' mango pulp. The developed method is precise, accurate, fast and able to maintain epoxy-carotenoids stability during analysis.

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Section: Resultssupporting

confidence: 82%

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Petry¹,

Mercadante²

2017

J. Braz. Chem. Soc.

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“…Although there are other good reversed-phase HPLC methods for determining carotenoids, retinoids, and sometimes tocopherols in plasma, these methods require more time and solvent usage (ranging from 32 to 70 min and 30 to 100 mL per sample) [19,20,22,32,48]. They also often use gradient HPLC, which requires more expensive systems than isocratic HPLC and more time for instrument re-equilibrium (although they can be useful for elaborate isomeric separations) [20,22,32,48].…”

Section: Rapid Isocratic Hplc Methods and Sample Extraction Proceduresmentioning

confidence: 99%

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Turner

Burri

2012

Chromatographia

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Vitamin A deficiency continues to be a major public health concern in the developing world effecting over 200 million people. Interventions including carotenoid-rich fruits and vegetables, containing a-and b-carotene, and b-cryptoxanthin, are being tested for their potential to alleviate vitamin A deficiency in several countries. Thus, there is a need for a faster isocratic reverse-phase high pressure liquid chromatography (HPLC) method than the methods currently available. Efficient extraction procedures for biological samples are also valuable to save time, materials, and cost. We developed an HPLC method and compared several extraction methods for carotenoids, retinoids, and vitamin E in plasma and human breast milk. The method uses an Agilent 1100 system equipped with a Waters Spherisorb ODS2 column (3 9 125 mm 3 lm) and similar guard column (ThermoScientific ODS2 3 9 20 mm 3 lm). Mobile phase of 7:2:1 acetonitrile:dichloromethane: methanol was run at 0.5 mL min -1 , and run times were complete in approximately 8 min. This rapid method uses little solvent and provides excellent results in the analysis of these fat-soluble nutrients. Analyte measurements were repeatable using standards and plasma and were accurate compared to NIST certified and reference serum values. The validated HPLC method was applied to the analysis of plasma samples from a vitamin A intervention study.

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“…Several HPLC methods for the analysis of retinol (R), retinyl palmitate (RP) and β-carotene (β-C) levels in tissues and blood serum are described, including UV/DAD [23,25,[27][28][29][30], fluorescence detection [31][32][33][34] and electrochemical detection [35][36][37][38]. Carotenoids and retinoids can be monitored by UV/ DAD detectors, retinoids by fluorescence detection, and carotenoids by electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

2010

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Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz [a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60 th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (p<0.001), (p<0,01), (p<0.05) in levels of retinol, retinyl palmitate and vitamin C, respectively compared to the control group levels. A significant decrease (p<0.01) in retinyl palimitate was observed in the 7,12 dimethylbenz [a] anthracene + Hypericum Perforatum L. treated group compared to the control group.. Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

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An improved HPLC method for determination of carotenoids in human serum

Cited by 44 publications

References 39 publications

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Rapid Isocratic HPLC Method and Sample Extraction Procedures for Measuring Carotenoid, Retinoid, and Tocopherol Concentrations in Human Blood and Breast Milk for Intervention Studies

Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

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