New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Petry, Fabiane C.; Mercadante, Adriana Zerlotti

doi:10.21577/0103-5053.20170127

Cited by 23 publications

(23 citation statements)

References 32 publications

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“…The APCI source operation parameters were as follows: ion source: turbo spray; source temperature: 350 °C; curtain gas (CUR): 25.0 psi; the collision gas (CAD): medium. DP and CE for individual MRM transitions were done with further DP and CE optimization ( Petry & Mercadante, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Exploring the differential stages of the pigment metabolism by pre-harvest bagging and post-harvest ethylene de-greening of Eureka lemon peel

Chen

Liu

et al. 2021

PeerJ

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Pre-harvest bagging or post-harvest ethylene treatments on lemons are commonly applied to change the surface color from green to favorable yellow. In this study, the differential mechanisms of the pigment metabolism by the two treatments were investigated by pigments contents and related genetic expression. The results showed that both treatments reduced the number of chloroplasts and the content of chlorophyll. The differential expression of PSY1 and PSY2 were observed, causing the different accumulation of the main carotenoid phytoene content. The differential expression of NYC resulted in altered contents of chlorophyll a and chlorophyll b, and further led to the difference in a* value. More interestingly, the degradation of chlorophyll uncovered the color of carotenoids, leading to the color changed from green to yellow.

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Section: Methodsmentioning

confidence: 99%

Exploring the differential stages of the pigment metabolism by pre-harvest bagging and post-harvest ethylene de-greening of Eureka lemon peel

Chen

Liu

et al. 2021

PeerJ

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“… a k = (t R − t 0 )/t 0 , where t R is the retention time of peaks while t 0 is the retention time of solvent peak, b α = (t R2 − t 0 )/ (t R1 − t 0 ) = k 2 /k 1 , where k 2 is the retention factor of peak 2 and k 1 is the retention factor of peak 1. c Numbers in parentheses represent peak numbers, d Average of duplicate analyses, e IS = internal standard, f Based on a report by Delgado-Pelayo, Gallardo-Guerrero and Hornero-Méndez [ 15 ], g Based on a report by Schex, et al [ 18 ], h Based on a report by de Faria, Hasegawa, Chagas, Pio, Purgatto and Mercadante [ 14 ], i Based on a report by Petry and Mercadante [ 19 ], j λ max = maximum absorption wavelength, k Based on report by Song, Li, He, Chen and Liu [ 17 ], l Based on a report by Liu, Chen, Kao and Shiau [ 16 ], and m Based on a report by Inbaraj, Lu, Hung, Wu, Lin and Chen [ 13 ]. …”

Section: Figurementioning

confidence: 99%

Inhibition of Melanoma Cells A375 by Carotenoid Extract and Nanoemulsion Prepared from Pomelo Leaves

Liu

Chen

2021

Plants

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This study aims to determine carotenoids in pomelo leaves (Citrus grandis Osbeck), a rich source of nutrients and phytochemicals, by high-performance liquid chromatography-mass spectrometry and prepare carotenoid nanoemulsions for the study of its inhibitory mechanism on melanoma cells A375. Fourteen carotenoids were separated within 27 min by using a YMC-C30 column and a gradient mobile phase of methanol-acetonitrile-water (84:14:2, v/v/v) and methylene chloride with a flow rate of 1 mL/min and detection wavelength of 450 nm. All-trans-lutein plus its cis-isomers were present in the largest amount (3012.97 μg/g), followed by all-trans-neoxanthin (309.2 μg/g), all-trans-violaxanthin (208.5 μg/g), all-trans-β-carotene plus its cis-isomers (203.17 μg/g), all-trans-α-carotene plus its cis-isomers (152.5 μg/g), all-trans-zeaxanthin (54.67 μg/g), and all-trans-β-cryptoxanthin plus its cis-isomers (24.56 μg/g). A stable carotenoid nanoemulsion was prepared with a mean particle size of 13.3 nm, zeta-potential of −66.6 mV, a polydispersity index of 0.132 and an encapsulation efficiency of 99%. Both the carotenoid extract and nanoemulsion could upregulate p53, p21, cyclin B and cyclin A expressions in melanoma A375 cells and downregulate CDK1 and CDK2 in a concentration-dependent manner. Also, they could upregulate Bax and cytochrome-C and downregulate Bcl-2, leading to cell apoptosis through activation of caspase-9, caspase-8 and caspase-3. Compared to extract, carotenoid nanoemulsion was shown to be more effective in inhibiting the growth of melanoma cells A375. This finding further demonstrated that a carotenoid nanoemulsion prepared from pomelo leaves possessed a great potential to be developed into functional foods or even botanic drugs.

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“…The pigmented phytoconstituents contained in palm kernel oil were quantified under the conditions described by [18], which consist in avoiding as much as possible after extraction, exposure of the oil to light, oxygen and acids. The pigment determination was done by the UV-Visible spectroscopic method [19].…”

Section: Content Of Chlorophylls (A and B) And Total Carotenoidsmentioning

confidence: 99%

Physico-chemical Quality Assessment of Palm Kernel Oil During Storage at Room Temperature and Shelter of Light

Viviane¹,

Bakoma²,

Mawuéna³

et al. 2019

IJPC

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Vegetable oils, including palm kernel oil, are an important part of the human diet. However, traditional methods of storing them often do not take into account changes in quality. In addition, these are food products whose rancidity by autooxidation could affect the health of the consumer. The objective of this work is to evaluate the physico-chemical quality of palm kernel oil during storage at room temperature and away from light. The palm kernel oil samples were collected in plastic containers under strict hygienic conditions, wrapped in aluminium foil and stored at ambient temperature away from light. Physico-chemical parameters were determined for fresh samples and for samples stored after 180 days. The results showed that after 180 days, the oils analysed showed the onset of oxidation. However, the characteristics after 180 days of storage indicate that the oils still have a relatively acceptable quality compared to Codex Alimentarius standards, concomitantly with the high content of saturated fatty acids. Thus, away from light, palm kernel oils could be stored for six months at ambient temperature with a lower risk of rancidity. However, the relative deterioration observed after 180 days of storage requires precautions in the conservation of palm kernel oils produced in Togo.

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New Method for Carotenoid Extraction and Analysis by HPLC-DAD-MS/MS in Freeze-Dried Citrus and Mango Pulps

Cited by 23 publications

References 32 publications

Exploring the differential stages of the pigment metabolism by pre-harvest bagging and post-harvest ethylene de-greening of Eureka lemon peel

Exploring the differential stages of the pigment metabolism by pre-harvest bagging and post-harvest ethylene de-greening of Eureka lemon peel

Inhibition of Melanoma Cells A375 by Carotenoid Extract and Nanoemulsion Prepared from Pomelo Leaves

Physico-chemical Quality Assessment of Palm Kernel Oil During Storage at Room Temperature and Shelter of Light

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