An immunocytochemical study of the endocrine pancreas in three genera of lacertids

Putti, Rosalba; Rossa, Angela Della; Varano, Lorenzo; Laforgia, Vincenza; Cavagnuolo, Antimo

doi:10.1016/0016-6480(92)90029-j

Cited by 17 publications

(18 citation statements)

References 27 publications

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“…Different from the present study, somatostatin-IR cells in the pancreatic islets of the reptilian species were located in the peripheral region of the 11 species of lacertids (Putti et al, 1992), desert lizard (El-Sahly et al, 1983) and anolian lizard (Rhoten and Smith, 1978). And also they were dispersed in the exocrine pancreas (Rhoten and Smith, 1978;El-Sahly et al, 1983;Putti et al, 1992). Somatostatin-IR cells showed the fourth highest frequency in the present study.…”

contrasting

confidence: 50%

“…Since PP-IR cells have been described for the first time in the lizard pancreas (Rhoten and Smith, 1978), the occurrence of these cells have been demonstrated in the pancreas of the reptiles (Rhoten and Smith, 1978;ElSahly et al, 1983;Agulleiro et al, 1985;GarcaAyala et al, 1987;Putti et al, 1992;Ku et al, 2000). From these previous results, PP-IR cells were dispersed in the exocrine pancreas in a case of the reptilian species but they showed different distribution according to sampling portions (El-Sahly et al, 1983).…”

mentioning

confidence: 75%

“…It was also reported that different regional distribution and relative frequency of endocrine cells in the pancreas were demonstrated in different portions even if they were (the) same pancreas of same lizard (Putti et al, 1992) and they showed speciesdependent characteristic distributional patterns in lizard species (El-Salhy and Grimelius 1981). In addition, more numerous and well-organized pancreatic endocrine systems are detected in the splenic lobes compared to that of duodenal lobes (El-Sahly and Grimelius, 1981).…”

Section: Original Papermentioning

confidence: 97%

“…Glucagon-IR cells were distributed in the peripheral mantle zone of the pancreas of 11 species of lacertids (Putti et al, 1992) and some of these IR cells were located in the central portions of the pancreatic islets. In the turtles, no glucagon-IR cells were detected in the central portion of the pancreatic islets (Perez-Tomas et al, 1989;Ku et al, 2000).…”

mentioning

confidence: 99%

“…And these IR cells were located in the islet center and comprised 3% of dorsal and 0.2% of ventral lobe volume in the squamate reptile, the desert lizard (El-Sahly et al, 1983). In addition, Putti et al (1992) reported that insulin-IR B cells appeared in 11 species, 3 genera of lacertids and different distributional patterns were seen in the pancreatic islets but these IR cells were mainly distributed in the central core of the pancreatic islets. From these previous reports (El-Sahly et al, 1983;Perez-Tomas et al, 1989;Putti et al, 1992;Morescalchi et al, 1997), insulin-IR cells that were the most predominant cell types in pancreas were located in the central core of the pancreatic islets, some cells were scattered in the inter acinar regions of the exocrine pancreas.…”

mentioning

confidence: 99%

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The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study

Hs²

2009

Eur J Histochem

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The regional distribution and frequency of the pancreatic endocrine cells in the splenic lobe of grass lizard, Takydromus wolteri, were studied by immunohistochemical (PAP) method using six types of specific mammalian antisera against bovine Sp-1/chromogranin (bCG), serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP). The pancreas was subdivided into two regions -islet kike and exocrine regions. The frequency of each immunoreactive (IR) endocrine cells was calculated as mean number/total 100 islet cells and as mean number/total 1000 cells (including exocrine and endocrine cells) using automated image analysis process. In addition, the percentage of each IR cell was also calculated. All of six endocrine cells were demonstrated. They were dispersed in the whole pancreatic parenchyma between exocrine acinar cells, or they were also observed as islet like clusters. In islet-like regions, bCG-, insulin-and glucagon-IR cells were detected as one or two cell layer cords and they were located between this cell-cords with 14.30±5.62, 61.50±9.76 and 26.50±9.31/100 cells frequencies, respectively. However, somatostatin-IR cells were mainly located in the peripheral parts not in cell-cords with 12.40±4.86/100 cells, and no serotonin-and hPP-IR cells were demonstrated. In exocrine regions, all of bCG-, serotonin-, insulin-, glucagon-, somatostatin-and hPP-IR cells were detected and they occurred mainly among the exocrine parenchyma as solitary cells with 10. 30±2.54, 0.80±0.63, 15.50±5.30, 5.80±2.66, 3.10±1.29 and 11.00±3.33/1000 cells frequencies, respectively. In addition, serotonin-IR cells were mainly located between epithelia and connective tissue of pancreatic duct. Overall, there were 0.58±0.49% serotonin-, 56.44±9.35% insulin-, 23.73±8.22% glucagon-, 11.28±3.03% somatostatin-and 7.97±2.02% hPP-IR cells

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confidence: 50%