Adrenomedullin in Nonmammalian Vertebrate Pancreas: An Immunocytochemical Study

López, Jesús García; Cuesta, Natalia; Cuttitta, Frank; Martı́nez, Alfredo

doi:10.1006/gcen.1999.7335

Cited by 37 publications

(21 citation statements)

References 47 publications

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“…Ultrathin sections (60-80 nm) were placed on nickel grids, etched for 5 min in 3% H 2 O 2 , and subjected to double immunogold staining as previously described (López et al 1999). Briefly, the sections were incubated in a mixture of both primary antibodies (anti-insulin and anti-factor H) at 4 C overnight.…”

Section: Electron Microscopymentioning

confidence: 99%

“…Pancreatic AM seems to have an early origin from both the phylogenetic and ontogenetic points of view. AM has been found in the pancreas of all vertebrate classes, exhibiting a complex pattern of colocalization with other pancreatic hormones, although in some cases it seems to identify a new cell type which contains AM only (López et al 1999). During embryonal development AM is also present from the very beginning of pancreatic differentiation, indicating some crucial function for this peptide hormone in embryogenesis (Martínez et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Expression of the adrenomedullin binding protein, complement factor H, in the pancreas and its physiological impact on insulin secretion

Martı́nez¹,

Pı́o²,

López³

et al. 2001

Journal of Endocrinology

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Adrenomedullin (AM) is a ubiquitous peptide hormone which, among other functional roles, reduces insulin secretion in the pancreas. Recently we have described the interaction between AM and the complement regulator protein factor H, which results in mutual modulation of their respective functions. Here we identify the expression of factor H in the cells of the rat pancreatic islets by immunohistochemistry and multiple immunofluorescence followed by confocal microscopy. In addition, double immunogold staining under the electron microscope showed coexistence of insulin and factor H immunoreactivities within the same secretory granules; interestingly, factor H staining was found in the electron-lucent haloes whereas the insulin antibody labeled preferentially the dense cores. The existence of factor H mRNA in the pancreas was confirmed by RT-PCR and in situ hybridization. The function of factor H in the pancreas was investigated with an insulin secretion assay. Addition of factor H to freshly isolated islets in the presence of AM resulted in a further reduction in insulin secretion with a concomitant elevation of cAMP, suggesting that factor H increases AM function in glucose homeostasis. The expression of factor H in the pancreas may play other important roles such as protection against complementmediated cell lysis.

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Section: Electron Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of the adrenomedullin binding protein, complement factor H, in the pancreas and its physiological impact on insulin secretion

Martı́nez¹,

Pı́o²,

López³

et al. 2001

Journal of Endocrinology

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“…Two considerations could be made. On the one hand, this lack of immunoreactivity cannot be attributed to the antisera or the immunocytochemical techniques used, since in a previous study, performed with the same antisera, techniques, and material belonging to the same specimens, immunoreactive cells for both antibodies were found in the pancreas (Ló pez et al, 1999;and unpublished observations). Second, although the juxtaglomerular cells are present in almost all the vertebrate groups (except in most elasmobranch fishes) and the distribution of renin contents present a similar pattern, the tinctorial characteristics of the secretory granules when exposed to such techniques as iron-hematoxylin, PAS-Alcian blue, and silver impregnation, are different between mammals and nonmammals (Sokoabe and Ogawa, 1974).…”

Section: Discussionmentioning

confidence: 85%

Proadrenomedullin N-Terminal 20 Peptide (PAMP) Immunoreactivity in Vertebrate Juxtaglomerular Granular Cells Identified by Both Light and Electron Microscopy

López

Cuesta

Martı́nez

et al. 1999

General and Comparative Endocrinology

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The gene for adrenomedullin (AM), a multifunctional peptide hormone, is expressed in mammalian renal tissue and has been shown to stimulate renin release. The exact cell source of this peptide and its gene-related partner, proadrenomedullin N-terminal 20 peptide (PAMP), in kidney is still uncertain. In the present study we have identified PAMP-immunoreactive cells in the kidney of different mammalian species, including man, by light microscopy. In addition, these cells have been further studied in mouse kidney by both light and electron microscopic techniques. At the light microscopic level, PAMP immunolabeling is preferentially located in the subendothelial cells of the enlarged glomerular afferent arterioles, that is, in the juxtaglomerular cells. However, these cells do not show immunolabeling for AM. At the electron microscopic level, the immunostaining appears inside the renin-containing secretory granules of the juxtaglomerular cells. These results confirm the direct link between renin and the AM peptide family and provide a morphological basis for studying the potential modulatory function of AM and PAMP in the control of renin activity. In contrast, neither AM nor PAMP immunoreactivities were detected in the kidney of nonmammalian vertebrates, other than in blood vessels of particular species, providing a new phylogenetic difference in the juxtaglomerular apparatus between mammalian and nonmammalian vertebrates. Academic Press

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“…The pancreas of elasmobranchs is a distinct compact organ that reflects an early state of combined exocrineendocrine relationship among vertebrates (Epple and Brinn 1987). Several studies of the endocrine pancreas (Sekine and Yui 1981;El-Salhy 1984;Conlon et al 1991;López et al 1999) using immunohistochemical techniques have revealed the existence of four endocrine cell types (A, B, D, and F) in the islets, as seen as in most bony fishes through to mammals. The FMRFa-IR cells were found as clusters in the islet tissue (Jönsson 1991).…”

Section: Discussionmentioning

confidence: 98%

“…Endocrine peptides that function in both the stomach and islet have been identified, the so-called GEP system (Jönsson 1995;López et al 1999;Kim et al 2000). The endocrine systems of elasmobranchs have been assumed to represent the primitive stage of differentiation in the evolution of the GEP endocrine system of vertebrates (Falkmer et al 1981).…”

Section: Discussionmentioning

confidence: 99%

An immunohistochemical study of Carassius RF amide in the stomach, intestine, and pancreas of the Japanese butterfly ray, Gymnura japonica

Inoue

Akiyoshi

2010

Ichthyol Res

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Carassius RFamide (C-RFa) is a brain-gut peptide, isolated originally from the brain of a teleost fish, Japanese crucian carp (Carassius auratus langsdorfii). It is a novel FMRFamide-related peptide sharing homologies with mammalian prolactin-related peptide. We observed the localization of C-RFa-like immunoreactivity (C-RFa-IR) in the stomach, intestine, and pancreas of Japanese butterfly ray using an immunohistochemical technique. In the whole gastrointestinal tract, C-RFa-IR was observed in smooth muscle cells, and in the Meissner's and Auerbach's plexus ganglia. Furthermore, in the stomach C-RFa-IR was also observed at the bottom of the fundic glands in both the body and pyloric region. In the intestine C-RFa-IR was observed in epithelial cells at the tip of mucosal folds. In the pancreas, C-RFa-IR was observed in islet cells. Thus, we suggest that ray C-RFa may have functional roles in both peripheral neurotransmission and endocrine function of the gastroenteropancreatic system.

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Adrenomedullin in Nonmammalian Vertebrate Pancreas: An Immunocytochemical Study

Cited by 37 publications

References 47 publications

Expression of the adrenomedullin binding protein, complement factor H, in the pancreas and its physiological impact on insulin secretion

Expression of the adrenomedullin binding protein, complement factor H, in the pancreas and its physiological impact on insulin secretion

Proadrenomedullin N-Terminal 20 Peptide (PAMP) Immunoreactivity in Vertebrate Juxtaglomerular Granular Cells Identified by Both Light and Electron Microscopy

An immunohistochemical study of Carassius RF amide in the stomach, intestine, and pancreas of the Japanese butterfly ray, Gymnura japonica

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