An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination

Zuidema, Dalen; Kerns, Karl; Šutovský, Peter

doi:10.3390/ani11123563

Cited by 28 publications

(24 citation statements)

References 75 publications

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“…Crossbreeding with improved exotic dairy breeds on a wide scale has been introduced as an option some many years back for upgrading the genetic potential of the indigenous cattle breed and later to enhance the dairy sector in the country [12]. Besides this, the creation and application of artifcial insemination (AI) techniques have altered the breeding of cattle and the genetic advancement of those animals, particularly in the dairy industry [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Reproductive Disorders of Cattle in the Tole District of Southwest Ethiopia and Their Prevalence and Associated Risk Factors

Tulu

Negera²

2022

Advances in Agriculture

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Reproduction is a basic requirement for efficient cattle production to continue or cease; whereas, reproductive disorders are the leading cause of economic loss in the cattle industry. To determine the prevalence and associated risk factors of major reproductive disorders in cattle in the Tole district, a cross-sectional study design was conducted from October, 2020, to October, 2021. To identify potential risk factors for cattle reproductive disorders, a multivariable random effect logistic regression analysis was used. A total of 400 randomly selected cows were examined, and 37.8% of them had at least one reproductive disorder. The three main reproductive disorders observed in the study area were abortion (17.8%), stillbirth (4.8%), and dystocia (3.3%). Risk factors for reproductive disorders in the study area included the calving season (OR = 3.7, 95% CL-1.37–7.18) and the accessibility of dogs to cattle (OR = 2.5, 95% CL-1.64–3.85). Accessibility of dogs to cattle in the study areas was also a risk factor for abortion (OR = 7.5, 95% CL-3.59–15.54). The present study also identified parity (OR = 5.2, 95% CL-1.05–26.04) as a risk factor for dystocia in cattle in the study areas. This demonstrated that losses in cattle production in the study areas are significantly attributed to reproductive problems. Hence, it is essential to increase public awareness of how reproductive disorders affect cattle production and to develop and implement appropriate control measures for these disorders. The causes of abortion and dystocia in various areas should be precisely identified and described through further research.

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Section: Introductionmentioning

confidence: 99%

Reproductive Disorders of Cattle in the Tole District of Southwest Ethiopia and Their Prevalence and Associated Risk Factors

Tulu

Negera²

2022

Advances in Agriculture

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“…Holden dan Butler (2018) menambahkan bahwa peningkatan populasi dan mutu genetik ternak dapat dilakukan dengan cara melakukan seleksi terhadap indukan jantan dan betina yang unggul dan sehat. Dengan menerapkan teknologi Inseminasi Buatan (IB) peternak dapat memperbaiki mutu genetik serta meningkatkan kualitas dan kuantitas ternak (Zuidema et al, 2021). Menurut Noakes et al (2018) IB merupakan salah satu cara untuk mengatasi keterbatasan jumlah pejantan unggul agar kapasitas reproduksinya dapat digunakan secara maksimal.…”

Section: Pendahuluanunclassified

The Effect of Sugarcane Water Subtitution as a Candidate Substitute for Fructose in CEP Diluent on Spermatozoa Quality of Friesian Holstein Bull during Frozen Storage

Rahayu

Ducha²

2022

JIPT

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Sugarcane water contains sucrose which function as an energy source to increase ATP and motility of sprematozoa. The purpose of this study was to determine the effect of sugarcane water substitution as a candidate for fructose substitute in CEP diluent on the sperm quality of Friesian Holstein (FH) bull in frozen storage. The type of research used was experimental research with Completely Randomized Design (CRD) with 5 treatments and 4 repetitions, namely A (CEP); B (CEP without fructose + 15% sugarcane water); C (CEP without fructose + 20% sugarcane water); D (CEP without fructose + 25% sugarcane water); E (CEP without fructose + 30% sugarcane water). Parameters used were motility, viability, and membrane integrity of FH bull spermatozoa. The motility test was observed with a 400x magnification microscope. The viability test was carried out with eosin-negrosin dye and observed with a 400x magnification microscope. The membrane integrity test was carried out with HOST fluid and observed with a 400x magnification microscope. Data analysis used the Kolmogorov-Smirnov test, then the One-Way ANOVA test and Duncan’s test. The result showed that the treatment with CEP diluent had the best results in maintaining the quality of spermatozoa during frozen storage with an average motility, viability and membrane integrity of 40,11±0,41%; 46,71±0,15% and 45,88±0,77% respectively.

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“…[17][18][19] This method has an improved fertilization rate; however, colloid centrifugation may not be applicable when the semen volume is relatively large. [20] Alternative sperm preparation methods have been developed to select sperm based on their morphological characteristics under high magnification, [21,22] maturity via HA bounding, [23,24] and membrane charge using electrophoresis, [25,26] or by removing apoptotic sperm using magnetic cell separation. [27][28][29] However, clinical application of these methods is still limited, mainly due to their highly manual procedures, harmful effect on sperm motility, dependence on clinician expertise, and lack of validation.…”

Section: Introductionmentioning

confidence: 99%

“…[ 17–19 ] This method has an improved fertilization rate; however, colloid centrifugation may not be applicable when the semen volume is relatively large. [ 20 ]…”

Section: Introductionmentioning

confidence: 99%

Sperm Syringe: 3D Sorting Platform for Assisted Reproduction

Parast

O'Bryan

Nosrati

2022

Adv Materials Technologies

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sperm injection (ICSI), [3] in vitro fertilization (IVF), [4] and intrauterine insemination (IUI). [5] In ICSI, as the most invasive method, an individual sperm is selected and injected directly into an egg. In IVF or IUI, a subpopulation of pre-selected sperm is introduced close to the egg to achieve fertilization. [3][4][5] The quality of selected sperm is crucial to ART, contributing significantly to the treatment success rate, live-birth rate, and offspring health. [6] However, the current clinical sperm selection practices are highly manual, subjective, and prone to operator errors, resulting in a suboptimal ART success rate, stagnating at ≈33% per cycle over the past 30 years. [7,8] The inherent challenge is to mimic the highly parallelized and 3D selection process in vivo that enables a single-cell level sorting mechanism within a relatively short timeframe. [9] Specifically, the 3D folded structure of the female reproductive tract breaks down the initial semen volume (≈1-4 mL) into a few microliters per selection event within the folded epithelial of the fallopian tube. These subfractioning events can process hundreds of millions of sperm in just a few hours. [10] Current clinical practices, however, only provide a 2D bulk scale selection alternative.Density gradient centrifugation (DGC) [11] and swim-up [12,13] are the most commonly used methods for human sperm selection in fertility clinics. In swim-up assay (SU), highly motile sperm are selected based on their ability to swim upward from the raw semen sediment into a fresh sperm media, where they are collected after ≈60 min. This method is not applicable in the case of asthenozoospermia (poor sperm motility). [14] In DGC, human sperm are separated based on their density by being forced to cross a viscosity gradient via centrifugation force that could cause sperm DNA damage and alter sperm morphology and function. [11,15] The sperm preparation method that is used in clinics is mainly chosen based on the count and motility of sperm in the human semen sample. [9] Both SU and DGC are time consuming (30-60 min), require multiple preparation steps, depend on the clinician expertise, and differ significantly from the natural selection process in vivo. [16] Moreover, the relatively long processing time of these methods and the low percentage of recovered sperm in the swim-up method (less than 20%) have considerably restricted the clinical workflow, especially Selection of high-quality sperm is crucial to assisted reproduction. However, conventional clinical methods for sperm selection are manual and prone to operator errors. This article presents 'sperm syringe', a scalable technology that mimics the highly parallelized 3D selection process in vivo via a 3D network of 560 microchannels to select high-quality sperm. Sperm syringe retrieves more than 41% of healthy sperm from the initial sperm sample in under 15 min, providing a sufficient volume (≈500 µL) and number (1,600,000) of high-quality sperm for fertility treatments. Experiments with bull and human sper...

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An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination

Cited by 28 publications

References 75 publications

Reproductive Disorders of Cattle in the Tole District of Southwest Ethiopia and Their Prevalence and Associated Risk Factors

Reproductive Disorders of Cattle in the Tole District of Southwest Ethiopia and Their Prevalence and Associated Risk Factors

The Effect of Sugarcane Water Subtitution as a Candidate Substitute for Fructose in CEP Diluent on Spermatozoa Quality of Friesian Holstein Bull during Frozen Storage

Sperm Syringe: 3D Sorting Platform for Assisted Reproduction

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