An experimental system for the production of antibodies in response to cryosurgical procedures

Experimental Production of Autoimmune Aspermatogenic Orchitis in the Rabbit in Consequence of in situ Freezing of the Testis

“…The cryosurgical procedure was basically identical to that employed and de tailed in earlier studies [7,8]. The basic differences being: firstly, in the target tis sue, i.e., the testis instead of the seminal vesicles and coagulating gland; secondly, the testis was frozen in situ 3 times at intervals of 30 days; and thirdly, freezing of the target area was accomplished hy controlling the flow of liquid-nitrogen to a 4-mm cryoprobe by a modified Linde CE-4 cryosurgical control unit (Linde Divi sion, Union Carbide [ 10J).…”

“…In view of previous studies demonstrating that in situ freezing of tis sue, i.e., the seminal vesicles and anterior prostate (coagulating gland) of the male rabbit constituted an antigenic stimulus [7][8][9], it was believed that in addition to attempting to demonstrate the possible production of antibodies following in situ freezing of other tissues, it was hypothesized that in situ freezing of the rabbit testis might provide not only the neces sary stimulus to evoke the production of antibodies but also, to be of sufficient magnitude as to induce the production of AIOA in the rabbit.…”

Immunotherapy for Prostatic Cancer

“…Perhaps the vital impetus directing attention to the potential use o f active immunotherapy in prostatic cancer were reports (44 -46) suggesting that obser vations o f regression o f metastatic lesions in patients following multiple in situ freezing o f their primary prostatic tumor were primarily on the basis o f the development of prostatic tissue-specific antibodies following experimental cryo surgery (47,48), attributable in part to augmentation o f host resistance, possibly o f an immunologic nature.…”

Immuno-Cryourogenital Treatment of Benign and Malignant Diseases of the Prostate

“…Studies initiated in our laboratory in 1965 demonstrated that in situ freezing of the prostatic tissue complex (seminal vesicles and coagu lating gland) of the male rabbit with a liquid nitrogen cooled probe resulted in the formation of circulating antibodies to the tissue frozen [17][18][19][20]. These antibodies were shown to be autoantibodies by virtue of the demonstration of positive in vitro reactions with extract prepa rations of the antibody-producer's own tissues [17][18][19][20].…”

“…These antibodies were shown to be autoantibodies by virtue of the demonstration of positive in vitro reactions with extract prepa rations of the antibody-producer's own tissues [17][18][19][20].…”