An evaluation of different microbial and rapid tests for determining inhibitors in milk

Evaluation of false positive results in microbial inhibitor tests for screening antibiotics in goat milk

“…Besides, at these concentrations, the pH of the milk samples was high, reaching pH of 9.82-10.80 for the highest concentrations tested, which could also inhibit the growth of the microorganism and thus, prevent the colour change of the test indicator system. These results agree with the results obtained by Zvirdauskiene and Salomskiene (2007) and Salomskiene et al (2013) who studied the effects of various commercial detergents on the microbial test response in cow milk, and found false positive results at alkaline detergent concentrations equivalent to the dose recommended by the manufactures and above. Although, it should be noted that these high concentrations are very unlikely to be found in practice, even with poor cleaning routines.…”

“…Žvirdauskiene and Salomskiene (2007) and Salomskiene et al (2013) found false positive results at alkaline detergent concentrations equivalent to the dose recommended by the manufactures and above. Also, these authors did not find interferences due to the presence of acid detergents in milk.…”

“…Some authors (Zvirdauskiene and Salomskiene, 2007;Salomskiene et al, 2013) only found false positive results at very high concentrations of alkaline detergents, equal or superior to the dose recommended by the manufacturers. However, Schiffmann et al (1992) obtained non-compliant results at lower concentrations (0.01%), whereas for these concentrations did not obtain any positive results, although they employed a limited number of cleaning products and microbial methods.…”

Determination of antibiotic residues in the pasteurized milk produced in West Azerbaijan province, North West of Iran

“…Studies by Žvirdauskienė and Šalomskienė compared the the tests such as LPT, MaI-1, Copan, Valio T 101, Penzym S, β-STAR, SNAP and ROSA tests showed that the Copan test was simple to use and interpret, had a long shelf life and was sensitive to all β-lactams [15] . Since the dairy factories rely on milk for production of yogurt and cream and dairy from the very first, thus milk containing antibiotics can cause economic losses.…”

Microbial based assay for specific detection of β-lactam group of antibiotics in milk

“…These methods either are qualitative, quantitative or semiquantitative and have one or more limitations in terms of precision, accuracy, sensitivity, cost and infrastructure requirement (Zvirdauskiene and Salomskiene 2007). Therefore, the present investigation was undertaken to develop a microbial assay for the specific detection of β-lactam antibiotics in milk based on induction of ß-lactamase (Davis et al 1974;Imsande et al 1972;Pollock 1950;Pollock 1961;Fonze et al 2002) in spore forming bacilli (Chen et al 2003;Kotiranta et al 2000;Drobniewski 1993).…”