An EPR study of manganese(II) binding to 5′-ATP, hemoglobin, and hemocyanin

Chang, Susan S; Li, Norman C.; Pratt, David W.

doi:10.1016/0022-2364(75)90229-2

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…In human blood, the majority of Mn 2+ ions are bound to protein, with about 80% bound to hemoglobin in erythrocytes and another 20% to proteins in plasma such as transferrin and globulin. 50,51 To free-up this protein-bound Mn (II) for electrochemical measurements, blood needs to be fully digested to mineralize the proteins to release all the Mn 2+ ions. Conventionally, blood is digested on a hot block in a strong oxidizer (e.g., nitric acid or a mixture of nitric acid and hydrogen peroxide) at elevated temperature, which mineralizes proteins and releases Mn 2+ .…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Determination of Manganese in Whole Blood with Indium Tin Oxide Electrode

Heineman

Haynes

et al. 2022

J. Electrochem. Soc.

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We demonstrate accurate and precise measurement of manganese (Mn) concentration in human whole blood with an indium tin oxide (ITO) electrode using square wave stripping voltammetry. While an essential trace metal for human health, elevated levels of Mn due to environmental or occupational exposure have been associated with severe neuromotor dysfunction characterized by parkinsonism and cognitive dysfunction making the monitoring of Mn in whole blood necessary. Pediatric populations are particularly susceptible to Mn given their developing brain and potential long-term impacts on neurodevelopment. The current gold standard for whole blood Mn measurements is by inductively coupled plasma-mass spectrometry, which is costly and time consuming. Electrochemical detection with an ITO working electrode showed a limit of detection of 0.5 µg L-1 and a linear range of 5 to 500 µg L-1 , which encompasses the physiological Mn levels in human whole blood. Our results show an average precision of 96.5% and an average accuracy of 90.3% compared to ICP-MS for both the normal range (5-18 µg L-1) and the elevated levels (>36 µg L-1) that require medical intervention. These results demonstrate the feasibility of Mn measurements in human blood with electrochemical sensors.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Determination of Manganese in Whole Blood with Indium Tin Oxide Electrode

Heineman

Haynes

et al. 2022

J. Electrochem. Soc.

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Reactions of Copper‐Free Hemocyanin from Taiwan Snails with Metal Salts

Wang

Chen

Tsay

et al. 1985

J Chinese Chemical Soc

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Copper‐free hemocyanin (apohemocyanin, apoHc) from Taiwan snails was prepared by cyanide treatment and the reconstitution of hemocyanin was carried out by treating apoHc with Cu(CH3CN)4ClO4. At pH below 5, the apoHc solution is cloudy. When reconstituted at pH near 6, the ratio of absorbances, A345/ a280, has the normal value 0.21, and rapidly decreases with increase in pH. This indicates that reconstitution is best carried out near pH 6. Addition of fluoride at pH 5.3 and cyanide at pH 5.7 causes a relatively rapid drop in absorbance at 345 nm, a primary reaction followed by a slower secondary process. Comparison of the reactions of native and reconstituted hemocyanins with oxygen, fluoride, and cyanide shows that the reconstitution has been successful. Cadmium and manganese derivatives of hemocyanin have been prepared. The order of affinity of metals to the active site of hemocyanin is: Cu>Mn>Cd.

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Dioxygen reactivity-structure correlations in manganese(II) complexes

Coleman¹

1980

Coordination Chemistry Reviews

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An EPR study of manganese(II) binding to 5′-ATP, hemoglobin, and hemocyanin

Cited by 3 publications

References 14 publications

Electrochemical Determination of Manganese in Whole Blood with Indium Tin Oxide Electrode

Electrochemical Determination of Manganese in Whole Blood with Indium Tin Oxide Electrode

Reactions of Copper‐Free Hemocyanin from Taiwan Snails with Metal Salts

Dioxygen reactivity-structure correlations in manganese(II) complexes

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