An EPR investigation of spin-labelled erythrocytes as a diagnostic technique for malignant hyperthermia

Cooper, P H; Kudynska, J.; Buckmaster, H. A.; Kudynski, R

doi:10.1016/0925-4439(92)90084-z

Cited by 13 publications

(7 citation statements)

References 26 publications

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“…The amount of doxyl stearates was added to the erythrocytes to obtain the optimal spin-label/membrane-lipid ratio of approximately 1:100. 45 EPR measurements. EPR spectra were recorded by using a Varian E104-A EPR spectrometer operating at X-band (9.1 GHz) and adjusted to the following settings: modulation amplitude, 2 G; modulation frequency, 100 kHz; microwave power, 10 mW; scan range, 100 G; scan time, 4 minutes; and time constant, 0.25 second.…”

Section: Gc-msmentioning

confidence: 99%

Fatty Acid Profiles of Four Wild Mushrooms and Their Potential Benefits for Hypertension Treatment

Mujić

Zeković

Vidović

et al. 2011

Journal of Medicinal Food

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Mushrooms are useful in balancing human diet and treating different health problems. The main determinant of the fluidity of erythrocyte membranes and rheologic properties of blood is the lipid composition of the membrane, which directly depends on lipids present in the diet. Lipid profiles of 4 European wild edible mushrooms, as well as the ability of mushroom lipids to modify the fluidity of erythrocyte membrane, were examined by using gas chromatography-mass spectometry, gas chromatography-flame ionization detector, and electron paramagnetic resonance spin probing technique. Lipids from 2 species--Macrolepiota procera and Collybia platyphylla--provoked an increase in erythrocyte membrane fluidity. Therefore, introduction of these and other wild mushrooms with similar lipid profiles to the human diet could be very beneficial in the treatment of hypertension and other cardiovascular diseases related to decreased fluidity of erythrocyte membranes.

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Section: Gc-msmentioning

confidence: 99%

Fatty Acid Profiles of Four Wild Mushrooms and Their Potential Benefits for Hypertension Treatment

Mujić

Zeković

Vidović

et al. 2011

Journal of Medicinal Food

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“…The average activation energies were This difference is expected because it was shown in the literature that deeper in the hydrocarbon region, where the nitrogen group of 16-DSA pene trates, exists a more fluid environm ent than com pared to that of the m em brane surface where the nitroxide group of 5-DSA probes (Berliner, 1976;Aszalos et al, 1984;Me Farland and Me Connell, 1971). Similarly, an increase in the m otional free dom of spin probes was reported in the literature (Minetti and Di Stasi, 1987;Wood et al, 1987;Cooper et al, 1992) for spin labels probing at the same mem brane depths as in our study on erythro cyte membranes. In biological m em branes the ro tational correlation time and order param eter, generally, decreases gradually as the depth of spin label from the polar surface of m em brane increases.…”

Section: Calculation O F Activation Energies and Phase Transition Temmentioning

confidence: 63%

In Vitro Effects of Colchicine on Human Erythrocyte Membranes: An ESR Study

Korkmaz

Aksöz

1997

Zeitschrift Für Naturforschung C

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“…The spin trap DEPMPO (5‐diethoxyphosphoryl‐5‐methyl‐1‐pyrroline‐ N ‐oxide) was purchased from Alexis Biochemical (Lausen, Switzerland). It was purified and tested using previously established procedures [29]. The membrane spin probe 7‐DS {7‐doxyl stearate [2‐(5‐carboxypentyl)‐2‐undecyl‐4,4‐dimethyloxazolidine‐3‐oxyl]}was purchased from Molecular Probes (Junction City, OR, U.S.A.).…”

Section: Methodsmentioning

confidence: 99%

Antioxidative capacity of C₆₀ (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes

Lens

Medenica

Citernesi³

2008

Biotech and App Biochem

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C(60) (buckminsterfullerene or fullerene C(60)) has been recognized an efficient free-radical scavenger. Owing to its high antioxidative capacity, C(60) and its derivatives have a great potential for application in biological systems where prevention of oxidative cell damage is desirable. However, poor solubility of native C(60) in water represents a major drawback for its use in biological systems. In order to increase the efficiency of delivery of fullerenes to target tissues it is of great interest to enhance their water solubility by developing hydrophilic organoderivatives of C(60) with retained antioxidative properties, and/or by encapsulating fullerenes in biocompatible liposomes. In the present study, using EPR spin-trapping and spin-labelling techniques, we investigated the antioxidative capacity of C(60) and newly synthesized fulleropyrrolidine derivatives Q-C(60) [N-methyl-(2-quinolyl)fulleropyrrolidine(60)] and I-C(60) [N-methyl-(2-indolyl)fulleropyrrolidine(60)] encapsulated in multilamellar phospholipid liposomes. The capacity for removal of (*)OH (hydroxyl radical) and O2(*-) (superoxide radical) and for the prevention of lipid peroxidation should be stressed as the most relevant biological antioxidative parameters. When these parameters for novel organofullerenes were compared with the the performance of C(60), Q-C(60) and I-C(60) showed similar, or even better, antioxidative characteristics. However, further research is required to establish the toxicity of these derivatives and their antioxidant efficacy in vivo.

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An EPR investigation of spin-labelled erythrocytes as a diagnostic technique for malignant hyperthermia

Cited by 13 publications

References 26 publications

Fatty Acid Profiles of Four Wild Mushrooms and Their Potential Benefits for Hypertension Treatment

Fatty Acid Profiles of Four Wild Mushrooms and Their Potential Benefits for Hypertension Treatment

In Vitro Effects of Colchicine on Human Erythrocyte Membranes: An ESR Study

Antioxidative capacity of C₆₀ (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes

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An EPR investigation of spin-labelled erythrocytes as a diagnostic technique for malignant hyperthermia

Cited by 13 publications

References 26 publications

Fatty Acid Profiles of Four Wild Mushrooms and Their Potential Benefits for Hypertension Treatment

Fatty Acid Profiles of Four Wild Mushrooms and Their Potential Benefits for Hypertension Treatment

In Vitro Effects of Colchicine on Human Erythrocyte Membranes: An ESR Study

Antioxidative capacity of C60 (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes

Contact Info

Product

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Antioxidative capacity of C₆₀ (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes