An Enzyme-Linked Immunosorbent Assay (ELISA) for Detecting Chicken Anti-Reovirus Antibody at High Sensitivity

Abstract

An enzyme-linked immunosorbent assay (ELISA) with high sensitivity was developed for use in the avian system. Anti-reovirus antibody produced in chickens against the isolants S1133, UMI-203, Reo-25, and Winterfield were detected with this method at high titers (up to 1:14,000).

Enzyme‐linked immunosorbent assay (ELISA) for detecting infectious laryngotracheitis viral antibodies in chicken serum

“…In poultry virology, this test has been shown to be of value to measure antibodies against infectious bronchitis virus (Mockett and Darbyshire, 1980;Soula and Moreau, 1981), reovirus (Slaght et al, 1978), infectious bursal disease virus (Marquardt et al, 1980;Howie and Thorsen, 1981), rotavirus (Bartz etal, 1980) and adenovirus (Dawson et al, 1980). The present paper describes the application of the ELISA to the detection of antibodies against infectious laryngotracheitis (ILT) virus in chicken serum.…”

An enzyme‐linked immunosorbent assay for measuring antibody titre against avian reovirus using a single dilution of serum

“…An indirect ELISA for detecting antibody to avian reovirus has been described by Slaght et al (1978). However, in our hands, this method did not appear to be readily adaptable without further modifications.…”

“…Antigen was diluted in distilled water (Slaght et al, 1978), or 0.05M carbonate-bicarbonate buffer, pH 9.6 (Voller et al, 1976) or NaOH solution, pH 13 (Miers et al, 1983), or 0.01M phosphate buffered saline, pH 7.5. Activated PVC plates containing antigen were incubated either for 1 hour at 37°C or overnight (18 hours) at 4°C.…”

“…Activated PVC plates containing antigen were incubated either for 1 hour at 37°C or overnight (18 hours) at 4°C. In addition to these, rigid polystyrene microtitre plates were pretreated with foetal calf serum (FCS) and glutaraldehyde and coated with antigen diluted in distilled water as described by Slaght et al (1978). All other steps were essentially the same as described under ELISA protocol.…”

“…Several methods of transforming absorbance values into antibody titre have been described (de Savigny and Voller, 1980;van Loon et al, 1981;Malvano et al, 1982;Snyder et al, 1983;Briggs et al, 1986;Hatfïeld et al, 1987), but no single method has been generally accepted. Slaght et al (1978) described an ELISA for detecting antibodies to avian reovirus in chicken serum. The present papeT compares several methods of predicting reovirus ELISA antibody titres in single dilutions of serum.…”