Enzyme‐linked immunosorbent assay (ELISA) for detecting infectious laryngotracheitis viral antibodies in chicken serum

Abstract

SUMMARYThe conditions of an indirect-enzyme linked immunosorbent assay for infectious laryngotracheitis virus (ILT) antibodies have been established. The specificity of the reaction was demonstrated.

Chicken anaemia virus antibody ELISA: Problems with non‐specific reactions

“…These results, which indicate non-specificity of some ELJSA reactions, confirm the experiences with other avian pathogens (Slaght et al, 1979;Meulemans & Halen, 1982;York et al, 1983] L. N. Payne, AFRC Institute for Animal Health, Compton, UK, personal communication, 1994). The results reported here indicate that the non-specific reaction occurs in a fraction (fraction 2) of serum not associated with antibody (IgG) reactions.…”

“…However, nonspecific reactions occur with IFA and the SN test requires substantial resources and time. The non-specific binding of chicken sera in ELJSAs has been attributed to many factors, such as high affinity for polystyrene of chicken immunoglobulins at neutral pH (Slaght et al, 1979;Meulemans & Halen, 1982). In the infectious laryngotracheitis virus antibody ELISA the non-specific factor could not be distinguished readily from normal chicken IgG and it has been postulated that it possesses many of the characteristics of hetero-antibody (York et.al., 1983).…”

Diagnosis of infectious laryngotracheitis using a monoclonal antibody ELISA

“…There was a clear distinction in OD readings between ELISA positive (minimum value 0.194) and ELISA negative samples (maximum value 0.099). DISCUSSION Although ELISAs have been described for the detection of antibody to ILT virus in chickens (Meulemans and Halen, 1982;York et al, 1983;Adair et al, 1985), there has been no previous report of the use of this technique for the detection' of ILT virus antigen. The ELISA described here, which uses a monoclonal antibody selected specifically for ELISA, was as sensitive as virus isolation for the detection of experimentally infected chickens.…”

Comparison of commercial ELISA test kits from Australia and the USA with the serum neutralization test in cell cultures for the detection of antibodies to the infectious laryngotracheitis virus of chickens

“…Another one is the unusually high af® nity of avian serum to plastic and polystyrene surfaces, which could lead to a high background activity (Slaght et al, 1978). According to Meulemans & Halen (1982) this non-speci® c reaction depends on factors such as the age of the chickens, on the quantity of IgM present in the serum and on the nature of the antigen coated on the wells. The latter authors tried to reduce these factors by employing a carbonatebicarbonate buffer instead of the PBS solution.…”