An environment-sensitive fluorescent probe for quantification of human serum albumin: Design, sensing mechanism, and its application in clinical diagnosis of hypoalbuminemia

Luo, Zijie; Liu, Bin; Zhu, Kangning; Huang, Yingying; Pan, Chengjun; Wang, Bingfeng; Wang, Lei

doi:10.1016/j.dyepig.2018.01.033

Cited by 46 publications

(15 citation statements)

References 48 publications

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“…In comparison with other detection methods, fluorescent probes are favored because of advantages including a rapid, non-invasive, and sensitive response. Accordingly, several fluorescent probes have been reported using many different types of fluorophore such as tetraphenylethylene, squaraine dye, and indolium derivative, which is based on aggregation-induced emission (AIE), molecular rotor containing donor-π-acceptor (D-π-A) structured fluorophore, and self-assembled nanoparticles [22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37]. However, there remain some problems to be solved, such as the low sensitivity and selectivity toward HSA.…”

Section: Introductionmentioning

confidence: 99%

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Lee

Sung

Kang

et al. 2019

Sensors

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The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Lee

Sung

Kang

et al. 2019

Sensors

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“…However, this bioprobe gave a detection limit that is lower than the reported detection limit of HSA (as low as 8.6 nM). 44 As far as we are concerned, little work has been done to identify and detect HSA using polymers as fluorescent probes, in particular "turn on" effect on the luminescence intensity. We also studied the detection of HSA in the presence of various biological interferents (Figure S6).…”

Section: Resultsmentioning

confidence: 99%

Macromolecular Probe Based on a Ni^II/Tb^III Coordination Polymer for Sensitive Recognition of Human Serum Albumin (HSA) and MnO₄^–

et al. 2019

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Reported here are the design and fluorescence characters of a Ni II /Tb III polymer, [Tb 2 Ni 3 (HCAM) 6 (H 2 O) 12 ] n ( 1 ) (H 3 CAM = chelidamic acid). Under physiological conditions, the binding of biocompatible water soluble 1 to human serum albumin (HSA) was studied by spectroscopy techniques, which revealed that 1 could inherent the fluorescent light of HSA in a static quencher course and change the HSA second-level structure. The marked enhancement in 1 and its fluorescence intensity provide conclusive evidence that 1 can play the role of a “turn-on” sensor for recognition and detection of HSA in other biological interferents with a K sv value of 7.68 × 10 4 M –1 and a detection limit of 0.14 μM. Luminescence experiments show that 1 has high selectivity and sensitivity to MnO 4 – in other anions. Its quenching efficiency ( K sv ) is 5.54 × 10 3 M –1 , and the detection limit is 0.29 μM.

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“…Fluorescence-based analytical methods enjoy advantages in terms of selectivity, sensitivity, simplicity, cost efficiency, and the in-time and on-site monitoring of the target. − Organic fluorescent probes have been developed based on different working mechanisms, mainly including (1) aromatic amides/esters which can undergo enzymatic hydrolysis in the presence of albumins due to albumin’s pseudo-esterase activity (Figure S1A, in Supporting Information), − (2) push–pull probes by modulating the twisted intramolecular charge transfer process between the nonpolar albumin binding sites and polar aqueous environment (Figure S1B), − (3) caged dyes or dark aggregates which can disassemble, disperse into albumin cavities, and turn on the emission (Figure S1C). − So far, many probes are not validated and applied in complex biological fluids except for the commercialized Albumin Blue Fluorescent Assays. − Nevertheless, these kits suffer from short shelf-lives because of their poor stability, and a specific fluorescence reader is additionally required due to the small Stokes shifts.…”

Section: Introductionmentioning

confidence: 99%

Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens

Yu²,

Zhou

et al. 2019

ACS Appl. Mater. Interfaces

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The analysis of albumin has clinical significance in diagnostic tests and obvious value to research studies on the albumin-mediated drug delivery and therapeutics. The present immunoassay, instrumental techniques, and colorimetric methods for albumin detection are either expensive, troublesome, or insensitive. Herein, a class of water-soluble tetrazolate-functionalized derivatives with aggregation-induced emission (AIE) characteristics is introduced as novel fluorescent probes for albumin detection. They can be selectively lighted up by site-specific binding with albumin. The resulting albumin fluorescent assay exhibits a low detection limit (0.21 nM), high robustness in aqueous buffer (pH = 6–9), and a broad tunable linear dynamic range (0.02–3000 mg/L) for quantification. The tetrazolate functionality endows the probes with a superior water solubility (>0.01 M) and a high binding affinity to albumin (K D = 0.25 μM). To explore the detection mechanism, three unique polar binding sites on albumin are computationally identified, where the multivalent tetrazolate–lysine interactions contribute to the tight binding and restriction of the molecular motion of the AIE probes. The key role of lysine residues is verified by the detection of poly-l-lysine. Moreover, we applied the fluorogenic method to quantify urinary albumin in clinical samples and found it a feasible and practical strategy for albumin analysis in complex biological fluids.

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An environment-sensitive fluorescent probe for quantification of human serum albumin: Design, sensing mechanism, and its application in clinical diagnosis of hypoalbuminemia

Cited by 46 publications

References 48 publications

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Macromolecular Probe Based on a Ni^II/Tb^III Coordination Polymer for Sensitive Recognition of Human Serum Albumin (HSA) and MnO₄^–

Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens

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An environment-sensitive fluorescent probe for quantification of human serum albumin: Design, sensing mechanism, and its application in clinical diagnosis of hypoalbuminemia

Cited by 46 publications

References 48 publications

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Macromolecular Probe Based on a NiII/TbIII Coordination Polymer for Sensitive Recognition of Human Serum Albumin (HSA) and MnO4–

Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens

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Macromolecular Probe Based on a Ni^II/Tb^III Coordination Polymer for Sensitive Recognition of Human Serum Albumin (HSA) and MnO₄^–