Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Lee, Suji; Sung, Dan-Bi; Kang, Seungyoon; Saravanan, P.; Choi, Jun Ho; Lee, Jong Seok; Han, Min Su

doi:10.3390/s19235298

Cited by 36 publications

(19 citation statements)

References 53 publications

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“…BSA is known to interact strongly with probes containing carboxylic groups. [42][43] Consistent with this trend, the carboxylate-containing probe 3D gave up to an 85fold increase in fluorescence when bound to BSA (data not shown), but the neutral ester-containing probe 4D only showed a minimal 3-fold increase (Fig. 3C).…”

Section: Resultssupporting

confidence: 65%

Visualizing the Dynamic Metalation State of New Delhi Metallo-β-lactamase-1 in Bacteria Using a Reversible Fluorescent Probe

et al. 2021

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New Delhi Metallo-β-lactamase (NDM) grants resistance to a broad spectrum of β-lactam antibiotics including last-resort carbapenems and is emerging as a global antibiotic resistance threat. Limited zinc availability adversely impacts the ability of NDM-1 to provide resistance, but a number of clinical variants have emerged that are more resistant to zinc scarcity (e.g., NDM-15). To provide a novel tool to better study metal ion sequestration in host-pathogen interactions, we describe the development of a fluorescent probe that reports on the dynamic metallation state of NDM within E. coli. The thiol-containing probe selectively coordinates the dizinc metal cluster of NDM and results in a 17-fold increase in fluorescence intensity. Reversible binding enables competition and time-dependent studies that reveal fluorescence changes used to detect enzyme localization, substrate and inhibitor engagement, and changes to metallation state through the imaging of live E. coli using confocal microscopy. NDM-1 is shown to be susceptible to demetallation by intracellular and extracellular metal chelators in a live-cell model of zinc dyshomeostasis, whereas the NDM-15 metallation state is shown to be more resistant to zinc flux. The development of this reversible turn-on fluorescent probe for the metallation state of NDM provides a new tool for monitoring the impact of metal ion sequestration by host defense mechanisms and to detect inhibitor target engagement during the development of therapeutics to counter this resistance determinant.

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Section: Resultssupporting

confidence: 65%

Visualizing the Dynamic Metalation State of New Delhi Metallo-β-lactamase-1 in Bacteria Using a Reversible Fluorescent Probe

et al. 2021

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“…In addition to antibody- and electrode-based biosensors, a fluorescence probe was recently developed [ 93 ]. The probe was capable of binding to major proteins in biological fluid, such as globulin, fibrinogen and transferrin.…”

Section: Biosensor Assays Of Liver Functionmentioning

confidence: 99%

Progress and Challenges in the Use of a Liver-on-a-Chip for Hepatotropic Infectious Diseases

Kulkeaw

Pengsart

2021

Micromachines

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The liver is a target organ of life-threatening pathogens and prominently contributes to the variation in drug responses and drug-induced liver injury among patients. Currently available drugs significantly decrease the morbidity and mortality of liver-dwelling pathogens worldwide; however, emerging clinical evidence reveals the importance of host factors in the design of safe and effective therapies for individuals, known as personalized medicine. Given the primary adherence of cells in conventional two-dimensional culture, the use of these one-size-fit-to-all models in preclinical drug development can lead to substantial failures in assessing therapeutic safety and efficacy. Advances in stem cell biology, bioengineering and material sciences allow us to develop a more physiologically relevant model that is capable of recapitulating the human liver. This report reviews the current use of liver-on-a-chip models of hepatotropic infectious diseases in the context of precision medicine including hepatitis virus and malaria parasites, assesses patient-specific responses to antiviral drugs, and designs personalized therapeutic treatments to address the need for a personalized liver-like model. Second, most organs-on-chips lack a monitoring system for cell functions in real time; thus, the review discusses recent advances and challenges in combining liver-on-a-chip technology with biosensors for assessing hepatocyte viability and functions. Prospectively, the biosensor-integrated liver-on-a-chip device would provide novel biological insights that could accelerate the development of novel therapeutic compounds.

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“…For in vivo applications, laser light source for excitation and two‐photon microscopy techniques will be useful. While a range of fluorescence probes have been reported for the detection of BSA or HSA, [36,47,48] far‐red fluorescence probe for the selective and differential detection of structural variants of BSA has not been documented. For instance, the extent of glycation and oxidation of BSA are found to be elevated in DM patients, and these structural alterations significantly impair the inherent binding affinity of BSA to a range of external ligands, including drugs and probes [49,50] .…”

Section: Figurementioning

confidence: 99%

Benzothiazole‐Phenothiazine Conjugate Based Molecular Probe for the Differential Detection of Glycated Albumin

Kumar

Datta

Samanta

et al. 2021

Israel Journal of Chemistry

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Glycation of albumin proteins is considered the pathophysiological hallmark of several chronic fatal disorders, including diabetes mellitus (DM) and Alzheimer's disease (AD). The optical detection of glycated albumin is a simple and cost‐effective tool with diagnostic implications for DM and AD. Herein, we developed a cyano derivative of the benzothiazole‐phenothiazine conjugate (TCNP) ‐based far‐red fluorescence probe for the differential detection of glycated bovine serum albumin (BSAG) over native BSA and oxidized BSA (BSAO). The selective fluorescence enhancement of TCNP in the presence of native BSA and BSAO and significant quenching in the presence of BSAG account for the distinct interaction between the probe and different structural variants of BSA. The high photostability and differential response towards native BSA and BSAG infer that TCNP is a potential molecular tool to assess the glycation induced structural changes of albumins.

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Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Cited by 36 publications

References 53 publications

Visualizing the Dynamic Metalation State of New Delhi Metallo-β-lactamase-1 in Bacteria Using a Reversible Fluorescent Probe

Visualizing the Dynamic Metalation State of New Delhi Metallo-β-lactamase-1 in Bacteria Using a Reversible Fluorescent Probe

Progress and Challenges in the Use of a Liver-on-a-Chip for Hepatotropic Infectious Diseases

Benzothiazole‐Phenothiazine Conjugate Based Molecular Probe for the Differential Detection of Glycated Albumin

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