An electrospun scaffold functionalized with a ROS-scavenging hydrogel stimulates ocular wound healing

Shi, Xin; Zhou, Tong; Huang, Shenyu; Yao, Yingming; Xu, Pinghong; Hu, Shaodan; Tu, Chenxi; Wang, Yin; Gao, Changyou; Ye, Juan

doi:10.1016/j.actbio.2023.01.016

Cited by 23 publications

(12 citation statements)

References 49 publications

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“…Although the plasma treatment changes the hydrophilicity of the material, its effect regresses with time and lasts only 3 h. 27 The incorporation of GelMA stably changes the scaffold from hydrophobic to hydrophilic, which is favorable for cells because hydrophilic materials can facilitate nutrient uptake and waste disposal. 28 The core−shell structure plays a crucial role in the microsolelectrospun fibers with delivering molecules. The images of TEM showed that a clear core−shell structure was successfully formed (Figure 1E).…”

Section: Scaffoldsmentioning

confidence: 99%

The “Inner–Outer” Molecules-Loaded Fiber-Hydrogel Scaffolds Coordinate Specific Immunity and Neural Differentiation for Repairing SCI

Hu,

Luo,

Chen

et al. 2024

ACS Materials Lett.

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The treatment of spinal cord injury (SCI) remains unsatisfactory because of the inflammatory microenvironment and the limited potential for nerve regeneration. However, most treatments have focused on modulating nonspecific immunity and ignored the importance of specific immunity in SCI. In this study, an "Inner− Outer" Metformin (Met) and anti-CD80 monoclonal antibody (CD80 mAb)-loaded fiber-hydrogel scaffold (P/G-Met-CD80 mAb) was constructed through microsol-electrospinning and UV illumination methods. The P/G-Met-CD80 mAb scaffolds exhibited good biocompatibility and hydrophilicity, facilitating cell proliferation and adhesion. In addition, the scaffolds were capable of releasing CD80 mAb and Met sequentially, exerting their protected functions at different stages post-SCI in rats. The released CD80 mAb at an early stage significantly inhibited specific immune response by blocking T-cell costimulatory activation and reducing IL-2 secretion, improving the inflammatory microenvironment after SCI. Late stage-released Met recruited neural stem cells (NSCs) to the injury area and enhanced NSCs differentiation into neurons. The P/G-Met-CD80 mAb scaffolds with specific immunomodulatory functions and neurogenic potential provide a new strategy for repairing SCI in the clinic.

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Section: Scaffoldsmentioning

confidence: 99%

The “Inner–Outer” Molecules-Loaded Fiber-Hydrogel Scaffolds Coordinate Specific Immunity and Neural Differentiation for Repairing SCI

Hu,

Luo,

Chen

et al. 2024

ACS Materials Lett.

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“…The Western blot analysis was also performed according to a previous study. 40 First, the accurately determined total protein content was extracted from cells using the BCA Protein Assay Kit (Beyotime Biotechnology, Shanghai, China). Second, the protein (40 μg) was separated using the 10% sodium dodecyl sulfate−polyacrylamide gel electrophoresis method and then transferred to poly(vinylidene difluoride) (PVDF) membranes (Hercules, CA).…”

Section: Tube Formation Analysis In Vitromentioning

confidence: 99%

A Multifunctional, Tough, Stretchable, and Transparent Curcumin Hydrogel with Potent Antimicrobial, Antioxidative, Anti-inflammatory, and Angiogenesis Capabilities for Diabetic Wound Healing

Fan,

Huang,

Zhang

et al. 2024

ACS Appl. Mater. Interfaces

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The treatment of diabetic chronic wounds is still faced with great challenges, mainly due to wound infection, excessive inflammation, and peripheral vascular disease in the wound area. Therefore, it is of great importance to develop a novel multifunctional hydrogel with high efficiency to accelerate diabetic wound healing. Curcumin (Cur), a Chinese herbal, has shown great potential in enhancing the healing of diabetic chronic wounds because of its immunomodulatory and pro-angiogenic properties. However, its low aqueous solubility, poor bioavailability, and chemical instability have limited its clinical applications. To address these current bottlenecks, novel poly(vinyl alcohol) (PVA)− chitosan (CS)/sodium alginate (SA)−Cur (PCSA) hydrogels were prepared for the first time, and they demonstrated all of the above intriguing performances by the Michael addition reaction of CS and Cur. PCSA hydrogels show multiple dynamic bonds, which possess strong mechanical properties (tensile stress: ∼0.980 MPa; toughness: ∼258.45 kJ/m 3 ; and compressive strength: ∼7.38 MPa at strain of 80%). These intriguing performances provided an optimal microenvironment for cell migration and proliferation and also promoted the growth of blood vessels, leading to early angiogenesis. Importantly, the experimental results demonstrated that PCSA hydrogels can effectively transform pro-inflammatory M1 macrophages into anti-inflammatory M2 macrophages without the need for additional ingredients in vitro. Benefiting from these characteristics, a full-thickness diabetic wound in a rat model demonstrated that PCSA hydrogels can effectively accelerate wound healing via ROS-scavenging, downregulation of IL-1β, and upregulation of CD31 expression, resulting in angiogenesis and collagen deposition. This strategy not only provides a simple and safe Cur-based hydrogel for diabetic wound healing but also highlights the significant potential for the development of high-performance biomaterials for promoting diabetic wound healing using traditional Chinese medicine.

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“…ROS, such as hydrogen peroxide (H 2 O 2 ), superoxide (O 2 ˙ − ), the hydroxyl radical (˙OH), and 1 O 2 , are not only strong oxidants but also important regulators of wound healing. 142 Celluar signalling requires low levels of ROS especially angiogenesis, which is one of the necessary conditions for wound healing. However, an excessive presence of ROS in wounds disrupts the balance of the oxidative/antioxidant system, resulting in oxidative stress.…”

Section: Multiple Functions Of Hydrogel-based Dressingsmentioning

confidence: 99%

Hydrogel-based dressings designed to facilitate wound healing

Zhang,

Liu,

Cheng

et al. 2024

Mater. Adv.

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An electrospun scaffold functionalized with a ROS-scavenging hydrogel stimulates ocular wound healing

Cited by 23 publications

References 49 publications

The “Inner–Outer” Molecules-Loaded Fiber-Hydrogel Scaffolds Coordinate Specific Immunity and Neural Differentiation for Repairing SCI

The “Inner–Outer” Molecules-Loaded Fiber-Hydrogel Scaffolds Coordinate Specific Immunity and Neural Differentiation for Repairing SCI

A Multifunctional, Tough, Stretchable, and Transparent Curcumin Hydrogel with Potent Antimicrobial, Antioxidative, Anti-inflammatory, and Angiogenesis Capabilities for Diabetic Wound Healing

Hydrogel-based dressings designed to facilitate wound healing

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