An Electrochemical Sensor Based on Gold Nanoparticles Incorporated in Mesoporous MFI Zeolite for Determination of Purine Bases in DNA

Abstract

An electrochemical sensor was developed based on gold nanoparticles incorporated in mesoporous MFI zeolite for the determination of purine bases. Au nanoparticles (AuNPs) were incorporated into the mesoporous MFI zeolite (AuNPs/m‐MFI) by post‐grafting reaction. The composite materials were characterized by transmission electron microscopy (TEM), X‐ray photoelectron spectroscopy (XPS) and electrochemical methods. Au nanoparticles with a size of 5‐20 nm are uniformly dispersed in the pores of mesoporous MFI zeolite. And the morphology of MFI zeolite can be perfectly kept after pore expansion and Au nanoparticles incorporation. The electrocatalytic oxidation of purine bases (guanine and adenine in DNA) is investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The surface‐confined Au nanoparticles provide the good catalytic activity for oxidation of purine bases. The simultaneous detection of guanine and adenine can be achieved at AuNPs/m‐MFI composites modified glassy carbon electrode (GCE). The electrochemical sensor based on AuNPs/m‐MFI exhibits wide linear range of 0.5–500 μM and 0.8–500 μM with detection limit of 0.25 and 0.29 μM for guanine and adenine, respectively. Moreover, the electrochemical sensor is applied to evaluation of guanine and adenine in herring sperm DNA samples with satisfactory results.

One‐step Synthesis of AuAg Alloy Nanodots and its Electrochemical Studies towards Nitrobenzene Reduction and Sensing

“…Fascinating electronic, optical, and chemical properties of gold (Au) and silver (Ag) nanoparticles (NPs), have received increasing care from the past decade and making them appropriate in a wide range of applications . In particular, they show characteristic size, shape, and composition dependent properties that are different from their bulk forms especially with the most significant catalytic properties observed when the particle size is in sub‐10 nm .…”

Electrochemical Sensor Platforms Based on Nanostructured Metal Oxides, and Zeolite‐Based Materials

“…Furthermore, it has been used for the detection of G and A in human blood serum and urine samples . Gold nanoparticles integrated in mesoporous MFI has been reported for the simultaneous determination of purine bases with a wide linear range of 0.5–500 μM and 0.8–500 μM with LOD of 0.25 and 0.29 μM for G and A, respectively . Au nanoparticles and mesoporous MFI zeolite displayed exceptional synergy effects for the detection of guanine and adenine.…”

“…Au nanoparticles and mesoporous MFI zeolite displayed exceptional synergy effects for the detection of guanine and adenine. The electrochemical sensor was used for the analysis of guanine and adenine in herring sperm DNA samples with satisfactory outcomes . TiO 2 nanoparticles‐magnesium doped zeolite Y modified carbon paste electrode has been fabricated for the simultaneous determination of G, A and T .…”

“…[168] Gold nanoparticles integrated in mesoporous MFI has been reported for the simultaneous determination of purine bases with a wide linear range of 0.5-500 mM and 0.8-500 mM with LOD of 0.25 and 0.29 mM for G and A, respectively. [169] Au nanoparticles and mesoporous MFI zeolite displayed exceptional synergy effects for the detection of guanine and adenine. The electrochemical sensor was used for the analysis of guanine and adenine in herring sperm DNA samples with satisfactory outcomes.…”

“…The electrochemical sensor was used for the analysis of guanine and adenine in herring sperm DNA samples with satisfactory outcomes. [169] TiO 2 nanoparticles-magnesium doped zeolite Y modified carbon paste electrode has been fabricated for the simultaneous determination of G, A and T. [170] This modified electrode displayed well-separated oxidation peaks toward G, A and T. Magnesium (II) loaded in zeolite matrix boosted anodic peak currents by the adsorption of G, A, and T compounds on the electrode surface. The excellent adsorptivity and conductivity of TiO 2 considerably enhanced the electrochemical efficiency.…”