An electrochemical label-free and sensitive thrombin aptasensor based on graphene oxide modified pencil graphite electrode

Ahour, F.; Ahsani, Mina

doi:10.1016/j.bios.2016.07.053

Cited by 74 publications

(26 citation statements)

References 40 publications

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“…[20][21][22][23][24] The fabrication of the working electrode with nanoparticles enhances peak current, sensitivity, reproducibility. 25,26 In current science and technology, one of the crucial uniqueness is to survey and to discover the new efficient materials as electrode surface modifiers.…”

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confidence: 99%

Electrochemical Sensor Based upon Ruthenium Doped TiO₂Nanoparticles for the Determination of Flufenamic Acid

Shetti

Nayak

Malode

et al. 2016

J. Electrochem. Soc.

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In the current research, ruthenium stood a conspicuous dopant for TiO 2 nanoparticles, to enhance its catalytic activity. The characterization of synthesized nanoparticles was accomplished by utilizing XRD, SEM, EDX and TEM analysis. The sensing surface morphology was studied by AFM analysis. Further, we established the electrochemical behavior and detection of flufenamic acid (FFA) by utilizing ruthenium doped TiO 2 nanoparticles modified carbon paste electrode (Ru-TiO 2 /CPE) at pH 6.0 by employing different voltammetric techniques. Modification enhances the electro-oxidation of flufenamic acid with increased current intensity. The influence of parameters like scan rate, pH, accumulation time, amount of the modifier and concentration on the peak current of the drug were studied. The effect of FFA concentration variation was studied using square wave voltammetric (SWV) technique and got lowest detection limit compared to reported techniques. The fabricated sensor was employed for the determination of flufenamic acid in biological samples. Flufenamic acid (FFA), N-(α,α,α-trifluoro-m-tolyl) an anthranilic acid derivative (Scheme 1), is an effective analgesic, an antiinflammatory and antipyretic agent used for the relief of pain and inflammation associated with musculoskeletal and joint disorders, peri-articular and soft tissue disorders, antirheumatic therapy.1,2 Monitoring of therapeutic drug habitually necessitates its detection in biological fluids.Many analytical methods based on different principles, such as spectrophotometry, 3-6 spectrofluorimetry, 5,11 fluorescence spectrometry, 7-10 flow injection analysis, 11,12 chromatography [13][14][15] have been developed for the analysis of flufenamic acid along with its derivatives. Capillary electrophoresis [16][17][18] is an alternative methodology for the separation and detection of FFA. However, these methods are well-demonstrated and broadly accepted, but they require moderately costly equipment, laborious sample pretreatment, specialized expertise, high cost and are time-consuming which make them unsuitable for routine analysis. Few voltammetric studies 5,19 were also carried out by utilizing cyclic voltammetry and adsorptive voltammetry. Though, these sensors suffer from the less sensitive, poor long-term stability, lack of surface renewability, etc.As of late, research developed, aiming at the construction and the characterization of the electrodes composed of carbonaceous materials because of their rich surface chemistry, lower background current, wider potential window, uncomplicated surface renewal and low detection limit properties. [20][21][22][23][24] The fabrication of the working electrode with nanoparticles enhances peak current, sensitivity, reproducibility. 25,26 In current science and technology, one of the crucial uniqueness is to survey and to discover the new efficient materials as electrode surface modifiers.In general, new types of sensor devices are the outcome of the synthesis and preparation of innovative modifiers. In this regard, as e...

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confidence: 99%

Electrochemical Sensor Based upon Ruthenium Doped TiO₂Nanoparticles for the Determination of Flufenamic Acid

Shetti

Nayak

Malode

et al. 2016

J. Electrochem. Soc.

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“…The detection limit for thrombin concentration (S/N=3) was estimated to be 0.35 fM. Compared to previous developed biosensors for thrombin detection such as electrochemical assay (70 pM), electrochemiluminescence assay (0.2 pM), chemiluminescence assay (6.2 pM), and colorimetric assay (0.68 pM), the designed PEC aptasensor still exhibited a lower detection limit as well as wider linear range, demonstrating its superiority. Additionally, in order to demonstrate synergistic effect of exonuclease aided target recycling, the sensitivity of the aptasensor just without RecJ exonuclease incubation was measured.…”

Section: Resultsmentioning

confidence: 99%

Signal‐On Photoelectrochemical Aptasensor Amplified by Exciton Energy Transfer and Exonuclease‐Aided Target Recycling

et al. 2017

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An enhanced photoelectrochemical (PEC) aptasensor was developed by coupling exciton energy transfer (EET) with exonuclease aided target recycling. Thrombin was selected as the target analyte. CdS quantum dots (QDs) as energy donors were first modified on the TiO2 film to form a TiO2/CdS heterostructure, and then complementary DNA (cDNA) of the thrombin aptamer probe (pDNA) was immobilized. Au nanoparticles (AuNPs) as energy acceptors were labeled at the 3′ end of pDNA to form AuNP‐pDNA composites. After pDNA hybridized with cDNA, the EET occurred and the photocurrent reduced evidently. When the mixture of thrombin and RecJ exonuclease was introduced, pDNA specifically bound with thrombin, leading to the separation of AuNP‐pDNA composites from the electrode. As RecJ exonuclease could cleave single‐strand pDNA from 5′–3′, the captured thrombin was set free and it recurrently binds with the rest of pDNA. As a result, the EET was broken, to a large extent, and the photocurrent clearly picked up. This signal‐on PEC aptasensor shows a low detection limit and good selectivity for target analysis.

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“…But everything is not ok with GFC based sensors high salt concentration is one of them. High salt concentration changes surface charge arrangement of GFCs by its aggregation and precipitation [29,30]. It is clear that the immense possibilities in terms of synthesizing Graphene and GFCs and fabricating functional bio-interfaces will lead to the fast development in this hot research area.…”

Section: Resultsmentioning

confidence: 99%

Bio-Sensing Applications of Graphene Based Composite Films

Maurya¹

2017

IJBSBE

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Graphene based composite materials have been extensively studied for the sensing applications attributing to their 2D structures, high conductivity, controlled modification and large specific surface areas, unique mechanical, optical, chemical, electrical, and catalytic properties. Therefore, a number of high quality sensors have been fabricated in recent years. Graphene based composite films (GCFs) that is base of such sensors can be prepared by combining Graphene with different functional nano-materials (carbon materials, noble metals, polymer materials, metal compounds etc.). In this review, we focus on the recent advances in bio-sensing applications of Graphene based composite films.

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An electrochemical label-free and sensitive thrombin aptasensor based on graphene oxide modified pencil graphite electrode

Cited by 74 publications

References 40 publications

Electrochemical Sensor Based upon Ruthenium Doped TiO₂Nanoparticles for the Determination of Flufenamic Acid

Electrochemical Sensor Based upon Ruthenium Doped TiO₂Nanoparticles for the Determination of Flufenamic Acid

Signal‐On Photoelectrochemical Aptasensor Amplified by Exciton Energy Transfer and Exonuclease‐Aided Target Recycling

Bio-Sensing Applications of Graphene Based Composite Films

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An electrochemical label-free and sensitive thrombin aptasensor based on graphene oxide modified pencil graphite electrode

Cited by 74 publications

References 40 publications

Electrochemical Sensor Based upon Ruthenium Doped TiO2Nanoparticles for the Determination of Flufenamic Acid

Electrochemical Sensor Based upon Ruthenium Doped TiO2Nanoparticles for the Determination of Flufenamic Acid

Signal‐On Photoelectrochemical Aptasensor Amplified by Exciton Energy Transfer and Exonuclease‐Aided Target Recycling

Bio-Sensing Applications of Graphene Based Composite Films

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Electrochemical Sensor Based upon Ruthenium Doped TiO₂Nanoparticles for the Determination of Flufenamic Acid

Electrochemical Sensor Based upon Ruthenium Doped TiO₂Nanoparticles for the Determination of Flufenamic Acid