An Electrochemical Impedimetric Aptasensing Platform  for Sensitive and Selective Detection of Small Molecules  Such as Chloramphenicol

Pilehvar, Sanaz; Dierckx, Tarryn; Blust, Ronny; Breugelmans, Tom; Wael, Karolien De

doi:10.3390/s140712059

Cited by 60 publications

(26 citation statements)

References 23 publications

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“…However, incubation with the glutaraldehyde showed a dramatic increase in Rct about 73% ( Figure 3 A, curve b) and indicates that the formation of linker on the modified electrode caused current passivation on the electrode surface and impede the electron movement. Moreover, on subsequent immobilization with the aptamer results in more increases of Rct value corresponding with a large curve diameter ( Figure 3 A, curve c) due to electrostatic repulsion force between negatively charged aptamers probe and redox couple [Fe(CN) 6 ] 3−/4− anions [ 36 ]. Subsequently, a drastic change of EIS signal showed 67% decrement of Rct can be seen after incubation of E2 ( Figure 3 A, Curve d), which indicates an immobilized aptamer successful bind specifically to the E2.…”

Section: Resultsmentioning

confidence: 99%

A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

et al. 2020

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A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples.

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Section: Resultsmentioning

confidence: 99%

A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

et al. 2020

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“…Pilehvar et al [162] developed an impedimetric electrochemical aptasensor for the detection of small molecules such as chloramphenicol. The Au electrode was modified with the specific aptamers by self-assembly.…”

Section: Chloramphenicolmentioning

confidence: 99%

Aptamer-based Biosensors for Antibiotic Detection: A Review

Mehlhorn

Rahimi

Joseph

2018

Preprint

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Antibiotic resistance and accordingly their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develope robust, easy, and sensitive methods for rapid evaluation of antibiotic and their residues. Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recent developed aptasensors for antibiotic detection. The use of various aptamer assays to determine different groups of antibiotics like β-lactams, Aminoglycosides, Anthracyclines, Chloramphenicol, (Fluoro)Quinolones, Lincosamide, Tetracyclines and Sulfonamides are presented in this paper.

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“…The physical continuity of PMPMS layers was investigated by monitoring electron transfer across the layer from ferri/ferrocyanide [Fe(CN) 6 ] 3−/4− anions in solution. Continuous films prevent the ions from approaching the gold surface, diminishing their redox activity [34]. These experiments used test solutions of 10 mM potassium ferri/ferrocyanide [Fe(CN) 6 ] 3−/4− redox couple in 200 mM KCl.…”

Section: Measurements Of Pmpms Continuitymentioning

confidence: 99%

Electrochemical Analysis of Ultrathin Polythiolsiloxane Films for Surface Biomodification

Chiang¹,

Levicky²

2018

International Journal of Electrochemistry

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The ability of different crosslinkers to crosslink nanometer thick films of the polymer poly(mercaptopropyl)methylsiloxane (PMPMS), thus stabilizing these films on solid supports, was investigated. The four crosslinkers included 1,11-bismaleimidotriethyleneglycol (BM(PEG)3), tris-(2-maleimidoethyl)amine (TMEA), bismaleimidohexane (BMH), and 1,1′-(methylenedi-4,1-phenylene) bismaleimide (BMDPM). PMPMS films treated with the four crosslinkers were compared in the effectiveness of achieved crosslinking, continuity and stability of the films to rearrangement at elevated temperatures, and modification with single-stranded DNA. The results of electrochemical analyses show that more hydrophilic crosslinkers had difficulty reacting fully with PMPMS thiols, even in these nanometer thin layers. This observation highlights the critical importance of selecting crosslinkers that are chemically compatible. Optimal selection of crosslinker yielded films in which the polymer film was largely incapable of rearranging, even at elevated temperatures, yielding reproducible and stable layers. These results validate use of these supports for applications such as monitoring thermal denaturation of immobilized DNA duplexes.

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An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

Cited by 60 publications

References 23 publications

A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

Aptamer-based Biosensors for Antibiotic Detection: A Review

Electrochemical Analysis of Ultrathin Polythiolsiloxane Films for Surface Biomodification

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