A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol

Abstract

<jats:p>A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10−7 to 1.0 × 10 −12 M, with a detection limit of 0.5 × 10−12 M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples.</jats:p>

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

“…The procedure was successfully applied to quantify E,E-DNL in QuEChERS extracts from Nile tilapia (Oreochromis niloticus) liver tissue with a recovery ranging from 92.3 to 98.8%. Haniphah [198] designed and assembled a simple and sensitive impedimetric aptasensor based on conductive carbon nanodots (CDs) to detect 17b-estradiol (E2). Carbon nanodots were electrodeposited on a screen-printed electrode (SPE), acting as a platform for immobilizing 76-mer aptamer probe.…”

“…Moreover, the average recovery rate for spiked river water samples ranged from 98.2% to 103.8%, evidencing the aptasensor possible application for E2 detection in water samples. [198].…”

“…Figure 12. Schematic representation of the immobilization strategy and hybridization detection of 17b-estradiol on aptamer/CDs/SPCE[198].…”

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

“…Electrochemical impedance spectroscopy (EIS) is a sensitive EC technique for evaluating charge transfer processes, such as double-layer capacitance, impedance and the solution resistance within the interface between an electrode and an electrolyte [114]. EIS provides some advantages as a sensing technique; for example, the samples are not destroyed during analysis, and it is simple to operate while providing a large amount of information that is obtained by detailed analysis of the data [115]. For instance, the charge transfer resistance (R CT ) of the Nyquist plot from EIS measurement is obtained from the Randles equivalent circuit that results from fitting the data [116].…”

Research developments in carbon materials based sensors for determination of hormones

“…Zaid et al [108] successfully analysed ED by a sensitive impedimetric aptasensor based on a screenprinted electrode /carbon nanodots/76-mer aptamer. The prepared electrode was characterized by UV-visible absorption spectra, fluorescence spectra, and transmission electron microscopy.…”