An electrochemical immunosensor for simultaneous multiplexed detection of neuron-specific enolase and pro-gastrin-releasing peptide using liposomes as enhancer

Zhong, Zhaoyang; Peng, Na; Qing, Ying; Shan, Jinlu; Li, Mengxia; Guan, Wei; Dai, Nan; Gu, Xianqing; Wang, Dong

doi:10.1016/j.electacta.2011.04.012

Cited by 29 publications

(18 citation statements)

References 20 publications

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“…Compared to other reported electrochemical immunosensors,43, 44 the proposed assay showed a lower detection limit. The high sensitivity could be attributed to the following reasons: The porous CaCO 3 sphere provided an efficient host for multiple AuNCs/HRP‐Ab 2 loading, which greatly enhanced the detection signal.…”

Section: Resultsmentioning

confidence: 73%

Calcium Carbonate–Gold Nanocluster Hybrid Spheres: Synthesis and Versatile Application in Immunoassays

Peng

Feng

Zhang

et al. 2012

Chemistry A European J

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Fluorescent gold nanoclusters (AuNCs) were incorporated into porous calcium carbonate spheres through electrostatic interaction. The resulting CaCO(3)/AuNCs hybrid material exhibited interesting properties, such as porous structure, excellent biocompatibility, good water solubility, and degradability. These properties make the CaCO(3)/AuNCs hybrid material a promising template to assemble horseradish peroxidase/antibody conjugates (HRP-Ab(2)). By using CaCO(3)/AuNCs/HRP-Ab(2) bioconjugates as probes, a versatile immunosensor was developed for fluorescent and electrochemical detection of the cancer biomarker neuron-specific enolase (NSE). The detection limits of the sensor were 2.0 and 0.1 pg mL(-1) for fluorescent and electrochemical detection, respectively. The immunosensor shows high sensitivity and offers an alternative strategy for the detection of other proteins and DNA.

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Section: Resultsmentioning

confidence: 73%

Calcium Carbonate–Gold Nanocluster Hybrid Spheres: Synthesis and Versatile Application in Immunoassays

Peng

Feng

Zhang

et al. 2012

Chemistry A European J

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“…In addition to the immunosensors discussed so far, Table 2 summarizes analytical information for other multiplexed immunosensing designs using labels of graphene sheets tagged with THI, Co(bpy) 3 3+ and Fc redox probes for the determination of AFP, CEA and streptococcussuis serotype 2 (SS2) [54], or AuNPs-modified Prussian blue (or nickel hexacyanoferrates) for PSA and free PSA [55], as well as liposomes with encapsulated ascorbic acid (AA) and uric acid (AU) for monitoring neuron-specific enolase (NSE) and pro-gastrin-releasing peptide (ProGRP), two biomarkers for small cell lung cancer [56]. Other recent methods used alginates of Cd, Pb and Cd for the determination of AFP, CEA and PSA by DPV [57], or Cd and Pb apoferritins for the stripping voltammetric detection of AFP and CEA by SWV [58], and metal ions-doped chitosan poly(acrylic) nanospheres for determining four cancer biomarkers, CEA, CA19-9, CA125 and CA242, in a single voltammetric run [59].…”

Section: Multiplexed Electrochemical Immunosensors Using Barcode Cmentioning

confidence: 99%

Multiplexed Electrochemical Immunosensors for Clinical Biomarkers

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

Sensors

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Management and prognosis of disease requires the accurate determination of specific biomarkers indicative of normal or disease-related biological processes or responses to therapy. Moreover since multiple determinations of biomarkers have demonstrated to provide more accurate information than individual determinations to assist the clinician in prognosis and diagnosis, the detection of several clinical biomarkers by using the same analytical device hold enormous potential for early detection and personalized therapy and will simplify the diagnosis providing more information in less time. In this field, electrochemical immunosensors have demonstrated to offer interesting alternatives against conventional strategies due to their simplicity, fast response, low cost, high sensitivity and compatibility with multiplexed determination, microfabrication technology and decentralized determinations, features which made them very attractive for integration in point-of-care (POC) devices. Therefore, in this review, the relevance and current challenges of multiplexed determination of clinical biomarkers are briefly introduced, and an overview of the electrochemical immunosensing platforms developed so far for this purpose is given in order to demonstrate the great potential of these methodologies. After highlighting the main features of the selected examples, the unsolved challenges and future directions in this field are also briefly discussed.

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“…Compared with the conventional single-analyte assay, simultaneous determination of multiple targets of interest has stimulated intense research, due to its remarkable advantages such as improved specificity and accuracy for diagnosis, shortened analytical time, high sample throughput, low sample requirement and reduced cost per assay [5][6][7]. In conclusion, multiplexed electrochemical immunoassay has become one of the leading trends, owing to its easy miniaturization and automated detection, high sensitivity, low cost, and good portability [8,9].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical coding for multiplexed immunoassays of biomarkers based on bio-based polymer-nanotags

Gan

et al. 2015

Electrochimica Acta

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An electrochemical immunosensor for simultaneous multiplexed detection of neuron-specific enolase and pro-gastrin-releasing peptide using liposomes as enhancer

Cited by 29 publications

References 20 publications

Calcium Carbonate–Gold Nanocluster Hybrid Spheres: Synthesis and Versatile Application in Immunoassays

Calcium Carbonate–Gold Nanocluster Hybrid Spheres: Synthesis and Versatile Application in Immunoassays

Multiplexed Electrochemical Immunosensors for Clinical Biomarkers

Electrochemical coding for multiplexed immunoassays of biomarkers based on bio-based polymer-nanotags

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