An electrochemical aptasensor for highly sensitive detection of zearalenone based on PEI-MoS2-MWCNTs nanocomposite for signal enhancement

Ma, Luyao; Bai, Lijuan; Zhao, Min; Zhou, Jing; Chen, Yongjie; Mu, Zhaode

doi:10.1016/j.aca.2019.02.012

Cited by 76 publications

(13 citation statements)

References 37 publications

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“…The MoS 2 NFs-PEI compound was obtained after centrifugation and washing with water for several times and dispersed in 2 mL of water. The modification of PEI not only increased water solubility of MoS 2 NFs [20], but also promoted the attachment to the protein [21,22]. Then 5 μL of glutaraldehyde (GA, 2.5%) and 6 μL of Ab 2 (3.7 mg mL −1 ) were injected to the obtained MoS 2 NFs-PEI solution, and the mixture was stirred for 3 h. During this reaction time, Ab 2 was immobilized on MoS 2 NFs-PEI hybrid through cross-linking reaction between PEI and Ab 2 .…”

Section: Preparation Of Mos 2 Nfs-ab 2 Nanoprobementioning

confidence: 96%

Graphene-PtPd nanocomposite for low-potential-driven electrochemiluminescent determination of carcinoembryonic antigen using Ru(bpy)32+

et al. 2021

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As well known, the electrochemiluminescence (ECL) of tris(2,2′-bipyridine)ruthenium(ii) (Ru(bpy) 3 2+ ) heavily relies on highly positive or negative triggered voltage, prejudicing the detection toward the bio-molecules. In this work, Ru(bpy) 3 2+ could generate enhanced and stable ECL at a low potential of 0.05 V (vs. Ag/AgCl) on graphene-PtPd hybrid, attributing to its excellent electrocatalysis from the synergistic effect between Pt and Pd. The obtained low-potential-driven ECL could be quenched by MoS 2 nanoflowers. Based on the quenching effect, a sandwich "signal-off" ECL immunosensor was fabricated to sensitively detect carcinoembryonic antigen (CEA). A linear calibration curve from 1 fg mL −1 to 1 ng mL −1 was obtained along with a low detection limit of 0.54 fg mL −1 (S/N = 3) under optimal conditions. The sensor showed satisfactory specificity, stability, and reproducibility and was successfully applied to determine CEA in actual samples. The recoveries ranged from 98.80 to 100.23%, and the relative standard deviation (RSD) was lower than 5%. Above all, this work explored new materials in low-potential-driven ECL system and provided a reliable sensing strategy for clinical applications.

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Section: Preparation Of Mos 2 Nfs-ab 2 Nanoprobementioning

confidence: 96%

Graphene-PtPd nanocomposite for low-potential-driven electrochemiluminescent determination of carcinoembryonic antigen using Ru(bpy)32+

et al. 2021

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“…After being doped with carbon nanotubes (CNTs), the MoS 2 -CNTs nanocomposites provide a larger specific surface area to immobilize more signal probes. Therefore, by employing Tb as the redox probe, Ma et al (2019) designed an EC aptasensor on a gold electrode for ZEN detection based on the nanocomposite of polyethyleneimine (PEI) functionalized MoS 2 doped multiwalled CNTs (PEI-MoS 2 -MWCNTs) for signal amplification (Figure 1d). In this design, numerous Tb signal probes were assembled on PEI-MoS 2 -MWCNTs nanocomposites to generate obvious and stable EC responses.…”

Section: Ta B L E 1 Mycotoxin Aptamer Sequencesmentioning

confidence: 99%

Aptasensors for mycotoxins in foods: Recent advances and future trends

Hou

Jia

Sheng

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxin contamination in foods has posed serious threat to public health and raised worldwide concern. The development of simple, rapid, facile, and cost‐effective methods for mycotoxin detection is of urgent need. Aptamer‐based sensors, abbreviated as aptasensors, with excellent recognition capacity to a wide variety of mycotoxins have attracted ever‐increasing interest of researchers because of their simple fabrication, rapid response, high sensitivity, low cost, and easy adaptability for in situ measurement. The past few decades have witnessed the rapid advances of aptasensors for mycotoxin detection in foods. Therefore, this review first summarizes the reported aptamer sequences specific for mycotoxins. Then, the recent 5‐year advancements in various newly developed aptasensors, which, according to the signal output mode, are divided into electrochemical, optical and photoelectrochemical categories, for mycotoxin detection are comprehensively discussed. A special attention is taken on their strengths and limitations in real‐world application. Finally, the current challenges and future perspectives for developing novel highly reliable aptasensors for mycotoxin detection are highlighted, which is expected to provide powerful references for their thorough research and extended applications. Owing to their unique advantages, aptasensors display a fascinating prospect in food field for safety inspection and risk assessment.

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“…Thiolated aptamer with urea immobilized on the Au by Au-SH binding. Then, dopamine was electropolymerized and urea removed EIS measurements of charge transfer resistance 0.005-0.1 nM, 1.00-500 nM, LOD 900 fM [189] Zearalenon Au electrode covered with PEI-MoS 2 -MWCNTs composite and Pt@Au nanoparticles followed by thiolated aptamer immobilization Toluidine blue (redox indicator) signal measured by cyclic voltammetry 0.5 pg/mL-50 ng/mL, LOD 0.17 pg/mL [190] This might result in a higher deviation of the signal and the necessity to use relative parameters such as the sight of the signal against blank experiment or against its maximal deviation within the analyte concentration range considered. The same can be referred to the use of surfactants and polyelectrolytes, which simplify the dispersion of CNTS and improve the stability of appropriate suspensions, especially those used as redox indicator carriers.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Advances in Electrochemical Aptasensors Based on Carbon Nanomaterials

et al. 2020

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Carbon nanomaterials offer unique opportunities for the assembling of electrochemical aptasensors due to their high electroconductivity, redox activity, compatibility with biochemical receptors and broad possibilities of functionalization and combination with other auxiliary reagents. In this review, the progress in the development of electrochemical aptasensors based on carbon nanomaterials in 2016–2020 is considered with particular emphasis on the role of carbon materials in aptamer immobilization and signal generation. The synthesis and properties of carbon nanotubes, graphene materials, carbon nitride, carbon black particles and fullerene are described and their implementation in the electrochemical biosensors are summarized. Examples of electrochemical aptasensors are classified in accordance with the content of the surface layer and signal measurement mode. In conclusion, the drawbacks and future prospects of carbon nanomaterials’ application in electrochemical aptasensors are briefly discussed.

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An electrochemical aptasensor for highly sensitive detection of zearalenone based on PEI-MoS2-MWCNTs nanocomposite for signal enhancement

Cited by 76 publications

References 37 publications

Graphene-PtPd nanocomposite for low-potential-driven electrochemiluminescent determination of carcinoembryonic antigen using Ru(bpy)32+

Graphene-PtPd nanocomposite for low-potential-driven electrochemiluminescent determination of carcinoembryonic antigen using Ru(bpy)32+

Aptasensors for mycotoxins in foods: Recent advances and future trends

Advances in Electrochemical Aptasensors Based on Carbon Nanomaterials

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