Graphene-PtPd nanocomposite for low-potential-driven electrochemiluminescent determination of carcinoembryonic antigen using Ru(bpy)32+

Abstract

As well known, the electrochemiluminescence (ECL) of tris(2,2′-bipyridine)ruthenium(ii) (Ru(bpy) 3 2+ ) heavily relies on highly positive or negative triggered voltage, prejudicing the detection toward the bio-molecules. In this work, Ru(bpy) 3 2+ could generate enhanced and stable ECL at a low potential of 0.05 V (vs. Ag/AgCl) on graphene-PtPd hybrid, attributing to its excellent electrocatalysis from the synergistic effect between Pt and Pd. The obtained low-potential-driven ECL could be quenched by MoS 2 nanoflowers. Based on the quenching effect, a sandwich "signal-off" ECL immunosensor was fabricated to sensitively detect carcinoembryonic antigen (CEA). A linear calibration curve from 1 fg mL −1 to 1 ng mL −1 was obtained along with a low detection limit of 0.54 fg mL −1 (S/N = 3) under optimal conditions. The sensor showed satisfactory specificity, stability, and reproducibility and was successfully applied to determine CEA in actual samples. The recoveries ranged from 98.80 to 100.23%, and the relative standard deviation (RSD) was lower than 5%. Above all, this work explored new materials in low-potential-driven ECL system and provided a reliable sensing strategy for clinical applications.

MnO2 Nanosheet/Polydopamine Double-Quenching Ru(bpy)32+@TMU-3 Electrochemiluminescence for Ultrasensitive Immunosensing of Alpha-Fetoprotein

“…2+ cannot be stable in aqueous solution and is difficult to directly apply to the ECL sensing platform. 16 Fortunately, MOF have been extensively studied by researchers due to their porosity, adjustable pore structure, and large specific surface area. 17 Among various MOF materials, more and more 3D MOF have been used as ideal carriers for Ru(bpy) 3 2+ immobilization.…”

“…Among them, Ru­(bpy) 3 2+ can be used as the most classical ECL light-emitting carrier due to its low excited state, high ECL quantum yield, and reversible electrochemical behavior . However, Ru­(bpy) 3 2+ cannot be stable in aqueous solution and is difficult to directly apply to the ECL sensing platform . Fortunately, MOF have been extensively studied by researchers due to their porosity, adjustable pore structure, and large specific surface area …”