2D graphene-based advanced nanoarchitectonics for electrochemical biosensors: Applications in cancer biomarker detection

Mukherjee, Soumajit; Mukherjee, Atripan; Bytesnikova, Zuzana; Ashrafi, Amir M.; Richtera, Lukas; Adam, Vojtech

doi:10.1016/j.bios.2024.116050

Cited by 10 publications

(3 citation statements)

References 163 publications

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“…It is possible to modify the surface of the nanocomposite to improve its sensitivity and selectivity in the detection of particular DNAs, miRNAs, enzymes, and proteins, which in turn allows for timely and precise diagnosis. According to reports, whole cancer cells with LOD values of approximately 10 cells, as well as DNAs, miRNAs, proteins, enzymes, and neurotransmitters with LOD values as low as 0.5 fM [ 294 ], can be detected. Similarly, Lee et al [ 295 ] discovered a novel electrochemical biosensor for detection of matrix metalloproteinase-1 (MMP-1), a potential biomarker for lung cancer.…”

Section: Electrochemical Sensing Of Biomoleculesmentioning

confidence: 99%

“…Furthermore, blood serum samples have a protein-rich environment that encourages the buildup of undesirable biomolecules on the biosensor surface, which impairs its functionality and eventually results in decreased sensitivity and stability. Addressing the biofouling effect has become a central focus for researchers [ 294 ]. For example, polymeric nanocomposites, such as Hb, Pcs, porphyrins, corroles, and molecularly imprinted polymers (MIP), are described as dependable materials to achieve the highest sensitivity and selectivity, and provide drug efficacy at various sites with quick, accurate detection.…”

Section: Electrochemical Sensing Of Biomoleculesmentioning

confidence: 99%

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Macromolecule–Nanoparticle-Based Hybrid Materials for Biosensor Applications

Kuntoji,

Kousar,

Gaddimath

et al. 2024

Biosensors

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Biosensors function as sophisticated devices, converting biochemical reactions into electrical signals. Contemporary emphasis on developing biosensor devices with refined sensitivity and selectivity is critical due to their extensive functional capabilities. However, a significant challenge lies in the binding affinity of biosensors to biomolecules, requiring adept conversion and amplification of interactions into various signal modalities like electrical, optical, gravimetric, and electrochemical outputs. Overcoming challenges associated with sensitivity, detection limits, response time, reproducibility, and stability is essential for efficient biosensor creation. The central aspect of the fabrication of any biosensor is focused towards forming an effective interface between the analyte electrode which significantly influences the overall biosensor quality. Polymers and macromolecular systems are favored for their distinct properties and versatile applications. Enhancing the properties and conductivity of these systems can be achieved through incorporating nanoparticles or carbonaceous moieties. Hybrid composite materials, possessing a unique combination of attributes like advanced sensitivity, selectivity, thermal stability, mechanical flexibility, biocompatibility, and tunable electrical properties, emerge as promising candidates for biosensor applications. In addition, this approach enhances the electrochemical response, signal amplification, and stability of fabricated biosensors, contributing to their effectiveness. This review predominantly explores recent advancements in utilizing macrocyclic and macromolecular conjugated systems, such as phthalocyanines, porphyrins, polymers, etc. and their hybrids, with a specific focus on signal amplification in biosensors. It comprehensively covers synthetic strategies, properties, working mechanisms, and the potential of these systems for detecting biomolecules like glucose, hydrogen peroxide, uric acid, ascorbic acid, dopamine, cholesterol, amino acids, and cancer cells. Furthermore, this review delves into the progress made, elucidating the mechanisms responsible for signal amplification. The Conclusion addresses the challenges and future directions of macromolecule-based hybrids in biosensor applications, providing a concise overview of this evolving field. The narrative emphasizes the importance of biosensor technology advancement, illustrating the role of smart design and material enhancement in improving performance across various domains.

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Section: Electrochemical Sensing Of Biomoleculesmentioning

confidence: 99%

Section: Electrochemical Sensing Of Biomoleculesmentioning

confidence: 99%

Macromolecule–Nanoparticle-Based Hybrid Materials for Biosensor Applications

Kuntoji,

Kousar,

Gaddimath

et al. 2024

Biosensors

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“…An electrochemical DNA biosensor is widely regarded as a precise, quick, inexpensive, and miniaturized technology, making it more suitable for on-site detection. − Such systems incorporate DNA probes as biorecognition elements for specific detection and quantification of targets designed by the Watson–Crick pairing principle. − Nanomaterials are often used as electrode materials to increase probe loading, accelerate the rate of electron transfer at the electrode surface, and thereby improve the sensitivity of electrochemical DNA biosensors. In 2 O 3 is a new semiconductor nanomaterial that has attracted attention for its low resistivity, high mobility, and good chemical stability. − N-doped carbon (NC) can effectively enhance the electron transfer efficiency of metal oxides .…”

Section: Introductionmentioning

confidence: 99%

In₂O₃/NC/Au NPs Nanocomposites for the Electrochemical Detection of Skeletonema costatum

Liu,

Chen,

Bin

et al. 2024

ACS Appl. Nano Mater.

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The outbreak and scale of harmful algal blooms (HABs) have been on the rise in recent years, mainly attributed to the effects of worsening seawater eutrophication and climatechange-related temperature increases. Yet, current approaches for detecting HABs fail to meet the requirements of rapid qualification and on-site quantification of the algae species and early warning of the outbreaks. Herein, an electrochemical biosensor based on In 2 O 3 /NC/Au NP nanomaterials was developed for the dynamic detection of Skeletonema costatum (S. costatum), one of the typical HABs. Specifically, the biosensor demonstrated a lower limit of detection (LOD, 671 fg/μL or 3528 cells/L) and had been confirmed to be accurate and reliable when compared to droplet digital PCR (ddPCR) and traditional microscope techniques. Moreover, for actual sample analysis, the concentrations of S. costatum were determined as 3.8 × 10 3 to 2.1 × 10 5 cells/L by the biosensor, which demonstrated a lower risk of S. costatum bloom outbreak in the sampling region and was consistent with the standard survey method. Therefore, the biosensor has great potential in the early stage qualitative analysis and on-site quantification of S. costatum and serves as an ideal warning technology of HAB outbreaks.

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Development of Ti₃C₂T_x‐based novel immunosensor for cancer biomarker detection

Sweety,

Kumar

2024

Applied Organom Chemis

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In this study, an ultrasensitive label‐free electrochemical immunosensor based on Ti3C2Tx (Mxene) with a 2D‐layered morphology has been proposed for the detection of EpCAM antigen. A hydrothermal method is employed for the synthesis of Ti3C2Tx by using a less toxic exfoliating reagent, NaBF4. The bioelectrode, BSA/anti‐EpCAM/Ti3C2Tx@ITO, has been fabricated by electrophoretic deposition of Ti3C2Tx onto the ITO electrode, followed by an immobilization of EpCAM antibody. Electrochemical response studies reveal that the immunosensor shows a high sensitivity of 29.22 μA fg−1 ml cm−2 and a wide linear range from 0.1 fg/ml to 100 ng/ml, for EpCAM antigen detection. Further, the good stability of this fabricated immunosensor is an additional advantage for EpCAM antigen detection in serum samples.

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2D graphene-based advanced nanoarchitectonics for electrochemical biosensors: Applications in cancer biomarker detection

Cited by 10 publications

References 163 publications

Macromolecule–Nanoparticle-Based Hybrid Materials for Biosensor Applications

Macromolecule–Nanoparticle-Based Hybrid Materials for Biosensor Applications

In₂O₃/NC/Au NPs Nanocomposites for the Electrochemical Detection of Skeletonema costatum

Development of Ti₃C₂T_x‐based novel immunosensor for cancer biomarker detection

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2D graphene-based advanced nanoarchitectonics for electrochemical biosensors: Applications in cancer biomarker detection

Cited by 10 publications

References 163 publications

Macromolecule–Nanoparticle-Based Hybrid Materials for Biosensor Applications

Macromolecule–Nanoparticle-Based Hybrid Materials for Biosensor Applications

In2O3/NC/Au NPs Nanocomposites for the Electrochemical Detection of Skeletonema costatum

Development of Ti3C2Tx‐based novel immunosensor for cancer biomarker detection

Contact Info

Product

Resources

About

In₂O₃/NC/Au NPs Nanocomposites for the Electrochemical Detection of Skeletonema costatum

Development of Ti₃C₂T_x‐based novel immunosensor for cancer biomarker detection