An efficient separation and method development for the quantifying of two basic impurities of Nicergoline by reversed-phase high performance liquid chromatography using ion-pairing counter ions

Do not just do it, do it right: urinary metabolomics –establishing clinically relevant baselines

“…HPLC‐NMR has been used successful for urine‐related metabolomics studies in rats (Akira et al ., ). Solid Phase Extraction (SPE) trapping interface allows online concentration and purification of very low‐level minor components (Godejohann et al ., ; Yalçın and Yüktaş, ; Spraul et al ., ). Although the comparison of NMR–spectral fingerprints is gaining popularity to understand differences between two sample sets, only a limited number of metabolomic studies have used NMR to compare human metabolomes (Lenz et al ., ; Kohl et al ., ).…”

Capillary electrophoresis–electrochemiluminescent detection of N,N-dimethyl ethanolamine and its application in impurity profiling and stability investigation of meclophenoxate

“…Most organic impurities in drugs are profiled by chromatographic methods of which reversed-phase HPLC [1][2][3][4][5] and thin layer chromatography (TLC) [6] coupled with UV or fluorescent detection have been the most popular for well over a decade. Other approaches reported for impurity profiling involve HPLC-mass spectrometry (MS), gas chromatography (GC) combined with flame ionization [7] or MS [8] detection, as well as some non-chromatographic strategies such as atomic absorption spectrometry [9], fluorimetry [10], UV [11], polarography [12] and nuclear magnetic resonance spectroscopy [13].…”