An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Paul, Santanu; Dam, Anandamoy; Bhattacharyya, A.; Bandyopadhyay, Tapas Kumar

doi:10.1007/s11240-010-9864-8

Cited by 54 publications

(28 citation statements)

References 35 publications

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“…TDZ has been proved to be a potential PGR and acts as a substitute for both the auxin and cytokinin requirements (Murthy et al, 1998). Plant regeneration by using TDZ was reported in several plants like Murraya koenigii (Paul et al, 2011) Jatropha curcas (Kumar and Reddy, 2012), Stevia rebaudiana (Lata et al, 2013). In some reports, TDZ was found to facilitate the shoot elongation and root induction (Debnath, 2005) by promoting regulated plant morphogenesis through the modulation of endogenous cytokinin and auxin (Gill and Saxena, 1992;Thomas and Katterman, 1986).…”

Section: Resultsmentioning

confidence: 99%

Efficient organogenesis from the induced meristemoid of Anthurium andraeanum Linden cv. Tinora

Bhattacharya¹,

Dam²,

Karmakar³

et al. 2015

Plant Sci Today

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We present here an efficient micropropagation protocol through direct regeneration of plants from meristemoids in Anthurium andraeanum Linden cv. Tinora. About 96.6±0.33 of in vitro grown nodal segments having axillary buds were induced to form meristemoids on modified MS basal medium supplemented with 0.92 µM Thidiazuron (TDZ). The significantly highest numbers of shoots (25.6±0.23) were regenerated from 93.3±0.33% of meristemoids in the same culture medium. The histological and scanning electron microscopic (SEM) study confirmed direct organogenesis from the meristemoid.

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Section: Resultsmentioning

confidence: 99%

Efficient organogenesis from the induced meristemoid of Anthurium andraeanum Linden cv. Tinora

Bhattacharya¹,

Dam²,

Karmakar³

et al. 2015

Plant Sci Today

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“…We have achieved 86% direct regeneration in EIM2 and obtained apparently normal plants in a short period of culture. Both factors have been described as convenient, to reduce the occurrence of somaclonal variation (Cote et al, 2001;Sharma et al, 2007;Paul et al, 2011). This is the first report that describes the use of mature seeds as explants for embryogenesis induction in grape.…”

Section: Discussionmentioning

confidence: 99%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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<p>The conservation of old grapevine varieties is important since they are adapted to specific climate conditions and may carry genes interesting to breeders. As virus infection is common in grapevine varieties, the use of virus free materials is of great importance. In this work, we used somatic embryogenesis for the sanitation of GLRaV-1 and GLRaV-3 viruses that were found after analyzing the putative presence of the five most common, economically important grape viruses by real-time multiplex RT-PCR in the old cultivar “Grumet Negre”. Unopened and opened inflorescences, fecundated ovaries, and, also, mature seeds were used as starting explants. Explants were<em> </em>cultured on plates with two embryogenesis induction media (Nitsch & McCown Woody plant medium) that contained the growth regulator thidiazuron and differed in their salt and vitamin compositions. One half of each kind of explant was cut prior to being cultured. After five months of culture, embryos had only developed from seeds that were cut previous to sowing. To the best of our knowledge, this is the first time that mature seeds have been used for inducing embryogenesis in grape. A total of 42% of the embryos transferred to tubes for germination regenerated into normal plantlets. The absence of both the GLRaV-1 and GLRaV-3 viruses in all regenerated plants was confirmed by real-time uniplex RT-PCR. So, this protocol can be used for sanitation and also for micropropagation.</p>

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“…In several plant species somatic embryogenesis is induced in the presence of auxin alone or in combination with cytokinins 24,25,26,27 and in a few systems cytokinins alone were found to be effective in somatic embryogenesis 28,29 . In this study it was found that the young shoot buds induced direct somatic embryogenesis at high BAP concentration (5 mg L-1 ) while its lower conc.…”

Section: Experimental Design Data Collection and Statistical Analysismentioning

confidence: 99%

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2016

IJPSR

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ABSTRACT:The study presents a protocol for mass propagation of Chlorophytum borivilianum through direct somatic embryogenesis. Direct somatic embryos occurred on the surface of juvenile shoot bud sprouts cultured on Murashige and Skoog medium supplemented with 5 mg L-1 BAP, yielding a high frequency of direct somatic embryos. On the other hand, a much lower concentration of BAP (0.5 mg L-1) led to the proliferation of somatic embryos. The conversion of somatic embryos with high MRN and MRL was favoured on BM. Encapsulation of somatic embryos (<5 mm) in calcium alginate beads resulted in the formation of well-formed synthetic seeds. Out of the synthetic seeds prepared from the somatic embryos (direct) using three types of gel matrices viz. DW, BM and SM, the ones formed using SM resulted in the highest frequency of germination (86.1%). Seed coat breakage and germination of synthetic seeds with the emergence of the tap roots were visible within 1-2 weeks of culture on basal medium. The plantlets obtained were successfully hardened on sand-soil-farmyard manure mixture with 85% survival rate. Hardened plantlets developed tubers comparable to the ones obtained in nature grown plants.

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An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

Cited by 54 publications

References 35 publications

Efficient organogenesis from the induced meristemoid of Anthurium andraeanum Linden cv. Tinora

Efficient organogenesis from the induced meristemoid of Anthurium andraeanum Linden cv. Tinora

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

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