Efficient organogenesis from the induced meristemoid of Anthurium andraeanum Linden cv. Tinora

Abstract

We present here an efficient micropropagation protocol through direct regeneration of plants from meristemoids in Anthurium andraeanum Linden cv. Tinora. About 96.6±0.33 of in vitro grown nodal segments having axillary buds were induced to form meristemoids on modified MS basal medium supplemented with 0.92 µM Thidiazuron (TDZ). The significantly highest numbers of shoots (25.6±0.23) were regenerated from 93.3±0.33% of meristemoids in the same culture medium. The histological and scanning electron microscopic (SEM) study confirmed direct organogenesis from the meristemoid.

The in vitro multiplication of anthurium cv. Eidibel

“…Na produção de mudas micropropagadas de antúrio, entre as citocininas utilizadas na fase de multiplicação, a 6-benzilaminopurina (BAP) tem sido mencionada como a mais eficiente na regeneração de brotos, com concentrações variando de 0,27 µM a 25,00 µM (ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al, 2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al, 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZANG et al, 2017;BHAVANA et al, 2018).…”

“…Several protocols are available in the scientific literature for the production of micropropagated plantlets of Anthurium andraeanum via organogenesis (ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al . ;2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al, 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZHANG et al, 2017).…”

“…Na literatura científica estão disponíveis vários protocolos para a produção de mudas micropropagadas de Anthurium andraeanum pela via organogênica (ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al;2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al, 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZHANG et al, 2017). Silva et al (2015) mencionam que o meio de cultivo MS (MURASHIGE;SKOOG, 1962) é o mais utilizado como meio basal na propagação in vitro de Anthurium.…”

“…The in vitro multiplication of anthurium cv. Eidibel Among the cytokinins used during the multiplication phase in the production of micropropagated anthurium plantlets, 6-benzylaminopurine (BAP) has been mentioned as the most efficient for shoot regeneration at concentrations ranging from 0.27 µM to 25.00 µM ( ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al, 2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al ., 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZANG et al, 2017;BHAVANA et al, 2018).…”

“…Studies on the production of micropropagated plantlets of anthurium 'Eidibel' are scarce, with recorded multiplication rates of from 3.95 to 6.11 (PINHEIRO et al, 2009). In addition, although the photoperiod of 12 h is used for the in vitro production of anthurium plantlets (GU et al, 2012;SAPTARI et al, 2017, ZANG et al, 2017, the most common is the photoperiod of 16 hours (ISLAM et al, 2010;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;GUADALUPE;MAYANIN, 2017;THOKCHOM;MAITRA, 2017;BHAVANA et al, 2018). The aim of this study, therefore, was to determine the most suitable relationship between growth medium, BAP concentration and photoperiod for increasing the in vitro multiplication rate of the 'Eidibel' cultivar.…”