An Efficient Micropropagation Protocol for High-Frequency Plantlet Regeneration from Liquid Culture of Nodal Tissues in a Medicinal Plant,<i>Turnera ulmifolia</i>L.

Shekhawat, Mahipal S.; Kannan, N.; Manokari, M.; Ramanujam, M. P.

doi:10.1080/10549811.2013.847793

Cited by 21 publications

(21 citation statements)

References 16 publications

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“…Multiple and healthy shoots were produced right after first subculture on this medium combination (Figures 1(a) and 1(b)). The results of in vitro regeneration on Acacia auriculiformis [22] and Turnera ulmifolia [23] also support the combined effects of BAP and Kn in shoot induction. The MS medium devoid of growth regulators did not show any regeneration capacity of axillary buds in the present study.…”

Section: Collection Of Data and Statistical Analysissupporting

confidence: 61%

EfficientIn VitroPropagation byEx VitroRooting Methods ofArtemisia absinthiumL., an Ethnobotanically Important Plant

Shekhawat¹,

Manokari²

2015

Chinese Journal of Biology

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Artemisia absinthiumis an important medicinal plant. Owing to the increasing anthropogenic activities and demand from the pharmaceutical industry, this plant species is overexploited; thereby this endangered its genetic stock in the wild. Therefore, it is urgently needed to develop nonconventional methods for conservation ofA. absinthium. Nodal segments obtained from the field grown 2-month-old plants were used as explants. Murashige and Skoog (MS) medium containing 0.5 mg/L 6-benzylaminopurine (BAP) and 0.25 mg/L kinetin (Kn) were reported to be optimum for induction of shoots (6.0 ± 0.52 shoots per explant). The shoots were multiplied by repeated transfer of original explants and by subculturing ofin vitroraised shoots on MS medium augmented with 1.0 mg/L each of BAP and Kn and 0.1 mg/Lα-naphthaleneacetic acid (NAA). Allin vitroregenerated shoots (100%) were rooted (4.4 ± 0.35 roots) on one-fourth strength MS medium supplemented with 2.0 mg/L indole-3 butyric acid (IBA). Cent percentage shoots rootedex vitroon sterile Soilrite under the greenhouse conditions when the shoots were treated with 200 mg/L of IBA for 5 min. Plantlets rootedin vitroandex vitrowere acclimatized successfully in the greenhouse and exhibited 87% and 95% survival rate.

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Section: Collection Of Data and Statistical Analysissupporting

confidence: 61%

EfficientIn VitroPropagation byEx VitroRooting Methods ofArtemisia absinthiumL., an Ethnobotanically Important Plant

Shekhawat¹,

Manokari²

2015

Chinese Journal of Biology

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“…The superiority of BAP over Kin in bud initiation from the explants has been proved in many plant species such as Holarrhena antidysenterica, Arnebia hispidissima, Randia dumetorum, Tectona grandis, M. citrifolia and Turnera ulmifolia (Ahmed et al, 2001;Ferdousi et al, 2003;Kumar et al, 2005;Shirin and Rana, 2005;Shekhawat et al, 2014;Sreeranjini and Siril, 2014). MS medium with BAP/Kin + IAA induced callus from the base of the explants and less number of shoots (7.1 and 4.8 shoots respectively) was regenerated from the nodal meristem of the explants.…”

Section: Resultsmentioning

confidence: 95%

In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

Shekhawat¹,

Kannan²,

Manokari³

2015

South African Journal of Botany

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An efficient micropropagation protocol has been developed successfully for Morinda coreia Buch.-Ham. by culturing nodal segments. The explants were washed, sterilized with HgCl 2 and inoculated on semi-solid Murashige and Skoog (MS) medium containing various concentrations and combinations of plant growth regulators (PGRs). Shoot bud initiation was observed after one week and 8.6 ± 0.32 shoots (per explant) harvested after five weeks on MS medium with 4.0 mg l −1 concentration of 6-benzylaminopurine (BAP). The regenerated shoots were further multiplied on semi-solid MS medium augmented with 2.0 mg l − 1 BAP + 1.0 mg l − 1 Kinetin (Kin). Maximum 24.5 ± 0.34 shoots per explant was obtained after five weeks on this media combination. The long (4-5 cm) and healthy shoots were rooted in vitro with 100% success rate on half strength MS medium + 1.0 mg l − 1 indole-3 butyric acid (IBA). Rooting and acclimatization were achieved simultaneously by ex vitro rooting method using 200 mg l − 1 IBA for 5 min with very good success rate (28.67 ± 05.51 roots per shoot with 100% response). The rooted shoots were transferred to the greenhouse for acclimatization for 4-5 weeks. The hardened plantlets were finally shifted to the field for further growth in the natural conditions after another five weeks. This is the first report on micropropagation of M. coreia, which can be successfully used for the large-scale multiplication and conservation of germplasm of this important medicinal plant.

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“…Cultures must be subcultured after 4 weeks in the liquid medium otherwise necrosis and tip burning starts and the leaves could turn yellow in color because of fast absorption of nutrients in liquid cultures. The multiplication of shoots in liquid media has several advantages, such as fast rate of multiplication, lack of impurities from agar, dilution of exudates from the explants, and uniform dispersal and better availability of nutrients and growth regulators (Lilia et al, 2002;Ascough and Fennell, 2004;Kalpana et al, 2009;Shekhawat et al, 2014). The liquid medium has been used successfully in various plants systems, especially for micropropagation of tree species (Dandin and Murthy, 2012;Rathore et al, 2014Rathore et al, , 2015.…”

Section: Tablementioning

confidence: 98%

Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

Shekhawat¹,

Kannan²,

Manokari³

et al. 2015

Journal of Applied Research on Medicinal and Aromatic Plants

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An Efficient Micropropagation Protocol for High-Frequency Plantlet Regeneration from Liquid Culture of Nodal Tissues in a Medicinal Plant,Turnera ulmifoliaL.

Cited by 21 publications

References 16 publications

EfficientIn VitroPropagation byEx VitroRooting Methods ofArtemisia absinthiumL., an Ethnobotanically Important Plant

EfficientIn VitroPropagation byEx VitroRooting Methods ofArtemisia absinthiumL., an Ethnobotanically Important Plant

In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

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