In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

Abstract

An efficient micropropagation protocol has been developed successfully for Morinda coreia Buch.-Ham. by culturing nodal segments. The explants were washed, sterilized with HgCl 2 and inoculated on semi-solid Murashige and Skoog (MS) medium containing various concentrations and combinations of plant growth regulators (PGRs). Shoot bud initiation was observed after one week and 8.6 ± 0.32 shoots (per explant) harvested after five weeks on MS medium with 4.0 mg l −1 concentration of 6-benzylaminopurine (BAP). The regenerated shoots were further multiplied on semi-solid MS medium augmented with 2.0 mg l − 1 BAP + 1.0 mg l − 1 Kinetin (Kin). Maximum 24.5 ± 0.34 shoots per explant was obtained after five weeks on this media combination. The long (4-5 cm) and healthy shoots were rooted in vitro with 100% success rate on half strength MS medium + 1.0 mg l − 1 indole-3 butyric acid (IBA). Rooting and acclimatization were achieved simultaneously by ex vitro rooting method using 200 mg l − 1 IBA for 5 min with very good success rate (28.67 ± 05.51 roots per shoot with 100% response). The rooted shoots were transferred to the greenhouse for acclimatization for 4-5 weeks. The hardened plantlets were finally shifted to the field for further growth in the natural conditions after another five weeks. This is the first report on micropropagation of M. coreia, which can be successfully used for the large-scale multiplication and conservation of germplasm of this important medicinal plant.

In vitro regeneration frequency, micro-morphological studies and ex vitro rooting of Hemidesmus indicus (L.) R. Br.: a multi-potent endangered climber

“…2B). The results are in agreement with the earlier reports in Lawsonia inermis , Celastrus paniculatus (Phulwaria et al 2013) and Morinda coreia (Shekhawat et al 2015a) etc. The higher concentrations of cytokinins than auxins in the culture medium could induce shoot organogenesis (Sharma and Singh 1997;Rout and Das 1997).…”

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

“…Celastrus paniculatus (Phulwaria et al 2013) and Morinda coreia (Shekhawat et al 2015a). It was observed that nodal segments with one node died due to the effect of HgCl 2 during surface sterilization but the middle nodes were survived even after HgCl 2 treatment when the nodal explants were used with 2-3 nodes.…”

Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers

“…When the ex vitro rooting of shoots and hardening of plantlets are achieved in a single step, the protocol takes a shorter time for plantlet production and become several times cheaper. Ex vitro root regeneration and simultaneous acclimatization was successfully proved by many researchers in Melothria maderaspatana (Baskaran et al, 2009), Agapanthus praecox (Baskaran and Van Staden, 2013), Caralluma edulis (Patel et al, 2014) and Morinda coreia (Shekhawat et al, 2015).…”